公开(公告)号：US20180113117A1

公开(公告)日：2018-04-26

申请号：US15791456

申请日：2017-10-24

Applicant: SYSMEX CORPORATION

Inventor： Keiko YOSHIKAWA ,  Yuma OKA ,  Shingo FUJIYAMA

IPC: G01N33/50 ,  G01N15/14 ,  G01N21/64

CPC classification number: G01N33/5041 ,  G01N15/1459 ,  G01N21/6428 ,  G01N33/566 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2021/6421 ,  G01N2021/6439 ,  G01N2333/705

Abstract: A cell information acquisition method may include: binding a binding substance to a receptor on a cell membrane surface of a cell, the binding substance being bindable to the receptor; permeabilizing a cell membrane of the cell after the binding; labeling a receptor in an intracellular area of the cell with a binding substance labeled with a first labeling substance after the permeabilizing; causing a specimen including the cell to flow through a flow path after the labeling; irradiating the cell included in the specimen flowing through the flow path with light; and acquiring a signal based on light generated from the first labeling substance in the cell irradiated with light.

公开(公告)号：US20200347452A1

公开(公告)日：2020-11-05

申请号：US16939193

申请日：2020-07-27

Applicant: SYSMEX CORPORATION

Inventor： Yuma OKA ,  Yuki AIHARA

IPC: C12Q1/6881

Abstract: A problem to be addressed is to provide a method that can evaluate the state of undifferentiated cells without destroying cells. The above problem is solved by providing a method for evaluating the state of undifferentiated cells. The method includes: measuring at least one miRNA selected from miR371, miR372, and miR373 in a liquid phase fraction of a liquid that contains undifferentiated cells; and evaluating a state of the undifferentiated cells on the basis of a measurement value of the miRNA.

公开(公告)号：US20210356454A1

公开(公告)日：2021-11-18

申请号：US17388223

申请日：2021-07-29

Applicant: SYSMEX CORPORATION

Inventor： Masatoshi YANAGIDA ,  Keiko MIWA ,  Yuma OKA

IPC: G01N33/50 ,  G01N33/58 ,  G06T7/00 ,  G01N15/14 ,  G01N33/49

Abstract: Disclosed is a method for measuring an immunostimulatory response of an immune cell, including (i) bringing a measurement target immune cell into contact with an immunostimulator, (ii) forming a contact surface with a substance different from the measurement target immune cell on the measurement target immune cell, (iii) bringing the measurement target immune cell into contact with a capturing body that binds to a surface antigen on the contact surface and is capable of generating an optical signal, (iv) detecting the optical signal generated from the capturing body, and (v) determining whether or not the measurement target immune cell from which the contact surface has been eliminated before detecting the optical signal has an immunostimulatory response, based on the detected optical signal.

公开(公告)号：US20210270812A1

公开(公告)日：2021-09-02

申请号：US17323444

申请日：2021-05-18

Applicant: SYSMEX CORPORATION

Inventor： Yuma OKA ,  Masatoshi YANAGIDA ,  Keiko MIWA

IPC: G01N33/50

Abstract: Disclosed is a method for analyzing immune cells, comprising the steps of mixing whole blood containing immune cells with an immunostimulatory factor in vitro and labeling target molecules of the immune cells, and a step of acquiring information on localization of the target molecules on the immune cells by detecting the label.