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公开(公告)号：US20190194710A1
公开(公告)日：2019-06-27
申请号：US16322518
申请日：2017-08-02
Applicant: Spiber Inc.
Inventor： Kenji Kurachi , Mihoko Kinoshita
CPC classification number: C12P21/00 , C12N1/00 , C12N5/0025 , C12N15/635 , C12P21/02
Abstract: The invention relates to a method for production of a recombinant protein using recombinant cells that express the recombinant protein under the control of an inducible promoter, the method including continuous culturing with addition of fresh culture medium to a portion of the culture solution in which the recombinant cells have been grown, wherein the recombinant cells whose expression of the recombinant protein has been induced are not reused.
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2.发明授权公开(公告)号：US12145971B2
公开(公告)日：2024-11-19
申请号：US16611315
申请日：2018-05-09
Applicant: Spiber Inc. , Kojima Industries Corporation
Inventor： Ayumi Abe , Keisuke Morita , Kenji Kurachi , Yunosuke Abe
Abstract: The present invention relates to a polypeptide solution in which a polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.
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3.发明申请公开(公告)号：US20200165311A1
公开(公告)日：2020-05-28
申请号：US16611315
申请日：2018-05-09
Applicant: Spiber Inc. , Kojima Industries Corporation
Inventor： Ayumi Abe , Keisuke Morita , Kenji Kurachi , Yunosuke Abe
Abstract: The present invention relates to a polypeptide solution in which polypeptide including an amino acid sequence derived from type I keratin that belongs to Cluster Ia is dissolved in a solvent including a formic acid or a solvent including an aprotic polar agent having a dipole moment of 3.0 D or more and an inorganic salt.
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