公开(公告)号：US20190194403A1

公开(公告)日：2019-06-27

申请号：US16328986

申请日：2017-09-01

Applicant: Spiber Inc. ,  Tekunohama Co., Ltd.

Inventor： Shinji Hirai ,  Yukimasa Ubukata ,  Ayumi Abe ,  Koichi Kotaka ,  Yuki Nakayama ,  Junichi Noba

IPC: C08J5/00 ,  B29C43/02 ,  B29C43/00 ,  C07K14/435

CPC classification number: C08J5/00 ,  B29C43/003 ,  B29C43/02 ,  B29K2089/00 ,  C07K14/435 ,  C07K14/43518 ,  C08H1/00 ,  C08J2389/00 ,  C08L89/00

Abstract: The present invention provides a method for producing a molded article including a step 1 of adding water to a composition including a structural protein, a step 2 of molding a water-containing composition obtained in the step 1, and a step 3 of drying the molded composition obtained in the step 2 to obtain the molded article.

公开(公告)号：US20190352330A1

公开(公告)日：2019-11-21

申请号：US16473836

申请日：2017-12-25

Applicant: Spiber Inc.

Inventor： Kazuhide Sekiyama ,  Akihiko Ozeki ,  Ryoji Okada ,  Koichi Kotaka

IPC: C07K1/14 ,  C07K14/435 ,  C08J11/08 ,  C08J11/20

Abstract: The present invention relates to a method for recovering protein, which recovers a target protein from a mixture containing the target protein and a material different from the target protein, the method including a dissolution step of dissolving either the target protein or the material by applying pressure while heating to a solution for dissolution containing the mixture and a polar solvent, and a separation step of separating an obtained solution.

公开(公告)号：US20220127755A1

公开(公告)日：2022-04-28

申请号：US17428136

申请日：2020-02-07

Applicant: Spiber Inc.

Inventor： Hirotada Ando ,  Hideto Ishii ,  Koichi Kotaka ,  Ryoko Sato ,  Hiroshi Kano ,  Jun Kobayashi ,  Akito Sato ,  Yunosuke Abe

IPC: D01D5/06 ,  C07K14/435 ,  D01F4/02

Abstract: An object of the present invention is to provide a method for producing an artificial structural protein fiber having a small diameter and having a stress equal to or higher than that of the related art. A method for producing an artificial structural protein fiber according to the present invention is a method for producing an artificial structural protein fiber by a wet spinning method, the method including a coagulation step of discharging a spinning dope containing an artificial structural protein and an organic solvent from a spinneret into a coagulation liquid to coagulate the artificial structural protein, wherein a bath draft in the coagulation step is more than 0.4 and 20 or less.

公开(公告)号：US20200207817A1

公开(公告)日：2020-07-02

申请号：US16633589

申请日：2018-07-25

Applicant: Spiber Inc. ,  Riken

Inventor： Keisuke Morita ,  Yunosuke Abe ,  Takehisa Maekawa ,  Koichi Kotaka ,  Junichi Sugahara ,  Keiji Numata

IPC: C07K14/435 ,  D01F4/02 ,  C12N15/63

Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)n motif-REP]m or Formula 2: [(A)n motif-REP]m-(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin. [In Formula 1 and Formula 2, (A)n motif represents an amino acid sequence consisting of 4 to 27 amino acid residues, and the number of alanine residues with respect to the total number of amino acid residues in the (A)n motif is 80% or more. REP represents an amino acid sequence consisting of 10 to 200 amino acid residues. m represents an integer of 10 to 300. A plurality of (A)n motifs may be the same amino acid sequence or different amino acid sequences.]

公开(公告)号：US20180355120A1

公开(公告)日：2018-12-13

申请号：US15780477

申请日：2016-11-29

Applicant: Spiber Inc. ,  Kojima Industries Corporation ,  Tekunohama Co., Ltd.

Inventor： Koichi Kotaka ,  Takaoki Ishii ,  Junichi Sugahara ,  Ryota Sato ,  Junichi Noba

IPC: C08J3/02 ,  C07K14/435 ,  C07K1/14

CPC classification number: C08J3/02 ,  C07K1/14 ,  C07K14/435 ,  C07K14/43586 ,  C08L89/00

Abstract: Provided is a method for producing a protein solution, including applying a pressure to a dispersion liquid containing a protein and a polar solvent in which the protein is dispersed, to obtain a protein solution containing the protein and the polar solvent in which the protein is dissolved.

公开(公告)号：US11692015B2

公开(公告)日：2023-07-04

申请号：US16633589

申请日：2018-07-25

Applicant: Spiber Inc. ,  Riken

Inventor： Keisuke Morita ,  Yunosuke Abe ,  Takehisa Maekawa ,  Koichi Kotaka ,  Junichi Sugahara ,  Keiji Numata

IPC: C07K14/435 ,  C12N15/63 ,  D01F4/02

CPC classification number: C07K14/43518 ,  C12N15/63 ,  D01F4/02

Abstract: The present invention relates to a modified fibroin including a domain sequence represented by Formula 1: [(A)nmotif-REP]m or Formula 2: [(A)nmotif-REP]m−(A)n motif, in which the domain sequence has an amino acid sequence with a reduced content of a glutamine residue equivalent to an amino acid in which one or a plurality of glutamine residues in REP are deleted or substituted with other amino acid residues, as compared with a naturally occurring fibroin.

公开(公告)号：US20210395317A1

公开(公告)日：2021-12-23

申请号：US17279564

申请日：2019-09-30

Applicant: Spiber Inc.

Inventor： Yunosuke Abe ,  Hirotada Ando ,  Koichi Kotaka

IPC: C07K14/435 ,  C07K14/47 ,  D01D5/06 ,  D01F4/00 ,  D01F4/02

Abstract: Disclosed is a method for producing a protein fiber, the method including: bringing a spinning dope containing a protein and an organic solvent into contact with a coagulation liquid to coagulate the protein, wherein a content of the protein in the spinning dope is more than 10% by mass based on a total amount of the spinning dope, and the coagulation liquid contains water or an aqueous solution of pH 0.25 or more and pH 10.00 or less.