公开(公告)号：US20230236105A1

公开(公告)日：2023-07-27

申请号：US17786163

申请日：2020-12-18

Applicant: Standard BioTools Canada Inc.

Inventor： Bedilu ALLO ,  Xudong LOU ,  Peng LIU ,  Daniel MAJONIS ,  Anastasia MAVROPOULOS ,  Ladan GHEIRATMAND

IPC: G01N15/14 ,  G01N33/483

CPC classification number: G01N15/1429 ,  G01N33/483 ,  G01N2015/1006

Abstract: Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to mass tags and additional reagents to reduce background and/or maintain target binding of mass tagged specific binding partners (SBPs), and schemes for associating a plurality of mass tags with a single SBP. As such, embodiments include any combination of one or more reagents and their use. The reagents, kits and methods herein may be used for mass cytometry, including imaging mass cytometry. In some aspects, reagents, kits or methods may be used for delivery of a large number of radioisotopes to a target analyte, for example for therapeutic use or radiometric detection. In certain aspects, only non-radioactive isotopes may be used for mass cytometry.