公开(公告)号：US20250138010A1

公开(公告)日：2025-05-01

申请号：US18692892

申请日：2022-09-22

Applicant: Standard BioTools Canada Inc.

Inventor： Colin Thom

IPC: G01N33/569 ,  G01N33/53 ,  G01N33/68

Abstract: Aspects of the subject application include applying a spatial barcode to a cellular sample and then performing suspension mass cytometry on the cellular sample. For example, a sample barcode comprising a known distribution of sample barcode isotopes may be applied to the sample such that cells in different locations receive a unique combination or ratio of barcode isotopes, after which cells are suspended (e.g., dissociated from tissue) and processed by suspension mass cytometry. While barcode isotopes are described in a number of embodiments herein, non-enriched elements may be used instead of, or in addition to, enriched isotopes. Mass cytometry methods and reagents are discussed below, followed by a further description of spatial barcoding and kits thereof.