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公开(公告)号：US11988632B1
公开(公告)日：2024-05-21
申请号：US18404846
申请日：2024-01-04
Inventor： Kai Jiang , Ping Wang , Yawei Tang , Tao Zhang
IPC: G01N27/44 , G01N27/447
CPC classification number: G01N27/44721
Abstract: Embodiments of the present disclosure provide a system for continuous scanning monitoring and analysis based on a discrete three-dimensional fluorescence technology. The system comprises a high-pressure capillary gel electrophoresis mechanism configured to enable passages of different lengths of STR sequence fragments and nucleic acid gene fragments in an energized state; a sampling window unit configured to assemble a plurality of capillary tubes and a plurality of detection optical fibers shared by an excitation light and excited light; and a detection window unit configured to assemble the plurality of detection optical fibers and a fluorescence signal detection unit. The fluorescence signal detection unit is configured to output a plurality of single excitation light sources and obtain fluorescence signals.
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公开(公告)号：US09869638B2
公开(公告)日：2018-01-16
申请号：US14760685
申请日：2014-07-23
Inventor： Kai Jiang , Yawei Tang , Ping Wang
CPC classification number: G01N21/6408 , G01N21/6428 , G01N2021/6413 , G01N2021/6439 , G01N2201/061 , G01N2201/12
Abstract: The present invention relates to a method for detecting time-resolved fluorescence based on a principle of phase balanced frequency multiplication modulation. A stimulating light source modulated by using a baseband signal acts on a to-be-measured target to trigger fluorescence, so that the fluorescence intensifies and decays periodically; then, a frequency-doubled square signal is used to control a sampling period and divide an ascending period of the fluorescence into two and a decay period of the fluorescence into two; after independent sampling is performed separately, sampling differences of the two parts are separately calculated and then added to obtain an intensity representative value of a fluorescence signal and to obtain a concentration value of the to-be-measured target. The method in the present invention can not only likewise cancel fluorescence interference of a substrate in a sample, but also can cancel ambient bias light, power-frequency interference of a spatial electromagnetic wave or other signals, and therefore improves signal intensity in fluorescence measurement on the detection sample, has an advantage that cannot be accomplished in a conventional time-resolved fluorescence method, and can be applied in fluorescence intensity detection of a target in fields such as biology, chemistry, and medicine.
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