公开(公告)号：US09903854B2

公开(公告)日：2018-02-27

申请号：US14632021

申请日：2015-02-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  John R. Waldeisen ,  Ivan Dimov ,  Benjamin M. Ross

IPC: G01N33/00 ,  G01N33/49 ,  G01N33/86

CPC classification number: G01N33/4905 ,  A61B5/14535 ,  A61B5/14546 ,  A61B5/150022 ,  A61B5/150099 ,  A61B5/150358 ,  A61B5/15142 ,  A61B5/157 ,  G01N33/491 ,  G01N33/86

Abstract: A point-of-test diagnostic chip that is capable of simultaneous evaluation of blood coagulation time (INR Value) and hematocrit level. A finger prick volume of blood is placed at the inlet of the device, and the residual vacuum contained within the device provides a pressure gradient to drive the flow of whole blood. The INR value is determined by the distance the blood traverses down the channel before it coagulates. The channel is primed with tissue factor and phospholipids to induce coagulation. A control channel is primed with anticoagulants for use as a way to normalize initial loading times. The hematocrit level is determined by sedimentation of blood cells into a trench and the interface between blood cells that have overfilled the trench and plasma provide a visual readout.

公开(公告)号：US20150233894A1

公开(公告)日：2015-08-20

申请号：US14632021

申请日：2015-02-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Luke P. Lee ,  John R. Waldeisen ,  Ivan Dimov ,  Benjamin M. Ross

IPC: G01N33/49 ,  G01N33/86

CPC classification number: G01N33/4905 ,  A61B5/14535 ,  A61B5/14546 ,  A61B5/150022 ,  A61B5/150099 ,  A61B5/150358 ,  A61B5/15142 ,  A61B5/157 ,  G01N33/491 ,  G01N33/86

Abstract: A point-of-test diagnostic chip that is capable of simultaneous evaluation of blood coagulation time (INR Value) and hematocrit level. A finger prick volume of blood is placed at the inlet of the device, and the residual vacuum contained within the device provides a pressure gradient to drive the flow of whole blood. The INR value is determined by the distance the blood traverses down the channel before it coagulates. The channel is primed with tissue factor and phospholipids to induce coagulation. A control channel is primed with anticoagulants for use as a way to normalize initial loading times. The hematocrit level is determined by sedimentation of blood cells into a trench and the interface between blood cells that have overfilled the trench and plasma provide a visual readout.

Abstract translation: 能够同时评估血液凝固时间（INR Value）和血细胞比容水平的点测试诊断芯片。 手指的血液体积被放置在装置的入口处，并且包含在装置内的残余真空提供压力梯度以驱动全血的流动。 INR值由血液凝固前通过通道的距离决定。 该通道用组织因子和磷脂引发以诱导凝血。 控制通道用抗凝剂灌注，用作标准化初始加载时间的方式。 通过血细胞沉积到沟槽中确定血细胞比容水平，并且已经超过沟槽和血浆的血细胞之间的界面提供了视觉读数。