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公开(公告)号：US11130993B2
公开(公告)日：2021-09-28
申请号：US15649328
申请日：2017-07-13
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , Jun Ho Son , Byungrae Cho
Abstract: Systems and methods for plasmonic heating by combined use of thin plasmonic film-based 2D and 3D structures and a light-emitting diode (LED) for nucleic acids amplification through fast thermal cycling of polymerase chain reaction (PCR) are described.
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公开(公告)号：US20180297028A1
公开(公告)日：2018-10-18
申请号：US15946615
申请日：2018-04-05
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , Erh-Chia Yeh
Abstract: A microfluidic apparatus and methods for fabrication with a fluidic layer and a pattern layer of spots of concentrated reagents that are disposed in wells of a fluidic layer when the two layers are bonded together. Reagents are stored on the chip prior to use. Because reagents are confined to specific wells, contamination of the channels and other microfluidic structures of the fluidic layer are avoided. The fluidic layer also has a system of vacuum channels and at least one vacuum void to store vacuum potential for controlled micro-fluidic pumping. The top and bottom surfaces of the bonded layers are sealed. The chip can be used for point of care diagnostic assays such as quantitative testing, digital nucleic acid amplification, and biochemical testing such as immunoassays and chemistry testing.
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公开(公告)号：US09970423B2
公开(公告)日：2018-05-15
申请号：US15454940
申请日：2017-03-09
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , Erh-Chia Yeh
CPC classification number: F04B19/006 , B01L3/50273 , B01L2300/0816 , B01L2300/0864 , B01L2300/0883 , B01L2400/049
Abstract: A fluidic chip employing a vacuum void to store vacuum potential for controlled micro-fluidic pumping in conjunction with biomimetic vacuum lungs.
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公开(公告)号：US09903854B2
公开(公告)日：2018-02-27
申请号：US14632021
申请日：2015-02-26
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , John R. Waldeisen , Ivan Dimov , Benjamin M. Ross
CPC classification number: G01N33/4905 , A61B5/14535 , A61B5/14546 , A61B5/150022 , A61B5/150099 , A61B5/150358 , A61B5/15142 , A61B5/157 , G01N33/491 , G01N33/86
Abstract: A point-of-test diagnostic chip that is capable of simultaneous evaluation of blood coagulation time (INR Value) and hematocrit level. A finger prick volume of blood is placed at the inlet of the device, and the residual vacuum contained within the device provides a pressure gradient to drive the flow of whole blood. The INR value is determined by the distance the blood traverses down the channel before it coagulates. The channel is primed with tissue factor and phospholipids to induce coagulation. A control channel is primed with anticoagulants for use as a way to normalize initial loading times. The hematocrit level is determined by sedimentation of blood cells into a trench and the interface between blood cells that have overfilled the trench and plasma provide a visual readout.
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公开(公告)号：US20190106664A1
公开(公告)日：2019-04-11
申请号：US15925445
申请日：2018-03-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Paul J. Hung , Philip J. Lee , Luke P. Lee
Abstract: Method and systems that provide improved handling and/or culturing and/or assaying of cells, chemically active beads, or similar materials in microfluidic systems and microfluidic culture arrays.
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Patent Agency Ranking
公开(公告)号：US09969963B2
公开(公告)日：2018-05-15
申请号：US14855584
申请日：2015-09-16
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Paul J. Hung , Philip J. Lee , Luke P. Lee
CPC classification number: C12M23/16 , B01L3/502707 , B01L3/502715 , B01L3/502746 , B01L3/502761 , B01L2200/027 , B01L2200/0647 , B01L2200/0689 , B01L2300/0627 , B01L2300/0816 , B01L2400/0487 , B01L2400/086 , B01L2400/088 , C12M21/08 , C12M23/34 , C12M29/06 , C12M29/10 , C12M41/46 , C12N5/0602
Abstract: Methods and systems are described for improved handling and/or culturing and/or assaying of cells, chemically active beads, or similar materials in microfluidic systems and microfluidic culture arrays.
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公开(公告)号：US20140342427A1
公开(公告)日：2014-11-20
申请号：US14288081
申请日：2014-05-27
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , Somin Eunice Lee
IPC: C12N13/00
CPC classification number: C12N13/00 , C12N15/111 , C12N2310/14 , C12N2320/30 , C12N2320/32 , Y10T428/2991
Abstract: A selectively addressable optical biomolecular carrier and its method of use for reconfiguring gene circuits are described. One carrier is a plasmon resonant nanoantenna formed from a gold metal nanorod coated with a cationic phospholipid bilayer with an aspect ratio between 2.0 and 8.0 and plasmon resonance wavelength in the near infrared range. Biomolecules such as siRNA adhere to the carrier and are introduced into a cell. The biomolecules are released from the nanoantenna carriers with exposure to light at the plasmon resonance wavelength. The nanoantenna efficiently converts absorbed optical energy to surface localized heat releasing the biomolecules at a time determined by the user. The carrier can be used to modify gene circuits by allowing temporal control over the genes within a selected gene circuit through the optical release of interfering nucleotides. Optical silencing of endogenous genes with siRNA released from nanoantenna carriers was used to illustrate the methods.
Abstract translation: 描述了一种选择性寻址的光学生物分子载体及其重组基因电路的方法。 一个载体是由涂覆有阳离子磷脂双层的金金属纳米棒形成的等离子体共振纳米天线，其长宽比在2.0和8.0之间，等离子体共振波长在近红外范围内。 生物分子如siRNA粘附于载体并引入细胞。 生物分子在等离子体共振波长处暴露于光下从纳米天线载体释放。 纳米天线有效地将吸收的光能转换成在用户确定的时间内释放生物分子的表面局部放热。 通过允许通过干扰核苷酸的光学释放对所选基因电路内的基因进行时间控制，载体可用于修饰基因电路。 使用从纳米天线载体释放的siRNA的内源基因的光沉默来说明方法。
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公开(公告)号：US11446667B2
公开(公告)日：2022-09-20
申请号：US16380693
申请日：2019-04-10
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , Jun Ho Sun
Abstract: An integrated molecular diagnostics system (iMDx) for the detection of Dengue Fever at point-of-care, wherein blood plasma separation for the selective separation of the Dengue virus from the whole blood, LED-driven photothermal lysis of the Dengue virus for the RNA extraction, and LED-driven rapid optical cavity PCR for the amplification of Dengue viral RNA are integrated on-chip within a single micro-fluidic device.
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公开(公告)号：US11406983B2
公开(公告)日：2022-08-09
申请号：US16938197
申请日：2020-07-24
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , Jun Ho Son
Abstract: An LED-driven optical cavity PCR system and method providing fast, accurate and reliable PCR based diagnostics. Thermal cycling for PCR takes place in an optical cavity formed in a microfluidic channel or chamber with thin light absorbing metal films disposed on at least two opposing walls that provide uniform heating between the walls by photothermal light to heat conversion. The opposing metal films may be sized for equal light absorption from a single light source orthogonal to the two opposing films to produce an even temperature elevation between the equally heating films upon a single light exposure. Uniform temperature elevation may also be achieved with films made from two different materials.
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公开(公告)号：US10766034B2
公开(公告)日：2020-09-08
申请号：US15878033
申请日：2018-01-23
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Luke P. Lee , Jun Ho Son
Abstract: An LED-driven optical cavity PCR system and method is disclosed for fast, accurate and reliable PCR based diagnostics. An optical cavity comprising two thin light absorbing metal (AU) films is used for uniform light absorption and subsequent photo thermal light-to-heat conversion is employed for PCR thermal cycling.
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