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    SELF-POWERED MICROFLUIDIC CHIP WITH MICRO-PATTERNED REAGENTS
    1.
    发明申请
    SELF-POWERED MICROFLUIDIC CHIP WITH MICRO-PATTERNED REAGENTS 审中-公开

    公开(公告)号：US20180297028A1

    公开(公告)日：2018-10-18

    申请号：US15946615

    申请日：2018-04-05

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Luke P. Lee ,  Erh-Chia Yeh

    IPC: B01L3/00 ,  B81B1/00 ,  B81C1/00

    Abstract: A microfluidic apparatus and methods for fabrication with a fluidic layer and a pattern layer of spots of concentrated reagents that are disposed in wells of a fluidic layer when the two layers are bonded together. Reagents are stored on the chip prior to use. Because reagents are confined to specific wells, contamination of the channels and other microfluidic structures of the fluidic layer are avoided. The fluidic layer also has a system of vacuum channels and at least one vacuum void to store vacuum potential for controlled micro-fluidic pumping. The top and bottom surfaces of the bonded layers are sealed. The chip can be used for point of care diagnostic assays such as quantitative testing, digital nucleic acid amplification, and biochemical testing such as immunoassays and chemistry testing.

    Digital fluid sample separation apparatus and methods for one-step quantitative sample analysis
    3.
    发明授权
    Digital fluid sample separation apparatus and methods for one-step quantitative sample analysis 有权

    公开(公告)号：US10589270B2

    公开(公告)日：2020-03-17

    申请号：US15017851

    申请日：2016-02-08

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Luke P. Lee ,  Erh-Chia Yeh

    IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6851 ,  C12Q1/689 ,  C12Q1/70 ,  B01L7/00 ,  G01N35/00

    Abstract: A Digital Separation (DS) chip for separating, digitizing and analyzing a fluid sample is presented. The DS chip includes a fluidic layer that prepares and compartmentalizes the fluid sample for analysis. Cliff structures that are adjacent to wells skim the fluid sample and prevent particles, which may interfere with fluid sample analysis, from entering the wells. Skimmed fluid sample analysis occurs in the wells and endpoint data can be collected and used to determine an original concentration of a desired component in the fluid sample very quickly. Using the described apparatus and methods, a fluid sample can be prepared, digitized, compartmentalized, assayed and the endpoint data collected in ˜30 minutes. The apparatus and methods can easily be adapted to provide parallel processing of a sample.

    DIGITAL FLUID SAMPLE SEPARATION APPARATUS AND METHODS FOR ONE-STEP QUANTITATIVE SAMPLE ANALYSIS
    4.
    发明申请
    DIGITAL FLUID SAMPLE SEPARATION APPARATUS AND METHODS FOR ONE-STEP QUANTITATIVE SAMPLE ANALYSIS 审中-公开
    数字流体样品分离装置及其一步数量化样品分析方法

    公开(公告)号：US20160228874A1

    公开(公告)日：2016-08-11

    申请号：US15017851

    申请日：2016-02-08

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Luke P. Lee ,  Erh-Chia Yeh

    IPC: B01L3/00 ,  C12Q1/68 ,  C12Q1/70

    Abstract: A Digital Separation (DS) chip for separating, digitizing and analyzing a fluid sample is presented. The DS chip includes a fluidic layer that prepares and compartmentalizes the fluid sample for analysis. Cliff structures that are adjacent to wells skim the fluid sample and prevent particles, which may interfere with fluid sample analysis, from entering the wells. Skimmed fluid sample analysis occurs in the wells and endpoint data can be collected and used to determine an original concentration of a desired component in the fluid sample very quickly. Using the described apparatus and methods, a fluid sample can be prepared, digitized, compartmentalized, assayed and the endpoint data collected in ˜30 minutes. The apparatus and methods can easily be adapted to provide parallel processing of a sample.

    Abstract translation: 提出了一种用于分离，数字化和分析流体样品的数字分离（DS）芯片。 DS芯片包括流体层，其准备和分隔流体样品用于分析。 与井相邻的悬崖结构清除流体样品并防止可能干扰流体样品分析的颗粒进入孔。 在孔中进行脱脂流体样品分析，并且可以收集终点数据并且非常快速地用于确定流体样品中所需组分的原始浓度。 使用所述的装置和方法，可以制备流体样品，数字化，分区化，测定，并在〜30分钟内收集终点数据。 该装置和方法可以容易地适应于提供样品的并行处理。

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