公开(公告)号：US20230280259A1

公开(公告)日：2023-09-07

申请号：US18117708

申请日：2023-03-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalali ,  Eric D. Diebold ,  Brandon Buckley

IPC: G01N15/14 ,  G01N33/537 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N33/537 ,  G01N2015/1006

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：US11630053B2

公开(公告)日：2023-04-18

申请号：US17666841

申请日：2022-02-08

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalali ,  Eric D. Diebold ,  Brandon Buckley

IPC: G01N15/14 ,  G01N33/537 ,  G01N21/64 ,  G01N15/10

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：US20230052995A1

公开(公告)日：2023-02-16

申请号：US17745206

申请日：2022-05-16

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Eric D. Diebold ,  Bahram Jalali ,  Brandon Buckley

IPC: G01N21/64 ,  G02B21/16 ,  G01N21/05 ,  G02B21/00 ,  G02B27/10 ,  G02F1/11

Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

公开(公告)号：US11371937B2

公开(公告)日：2022-06-28

申请号：US16528463

申请日：2019-07-31

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Eric D. Diebold ,  Bahram Jalali ,  Brandon Buckley

IPC: G01N21/64 ,  G02B21/16 ,  G01N21/05 ,  G02B21/00 ,  G02B27/10 ,  G02F1/11

Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

公开(公告)号：US11327016B2

公开(公告)日：2022-05-10

申请号：US16528436

申请日：2019-07-31

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Eric D. Diebold ,  Bahram Jalali ,  Brandon Buckley

IPC: G01N21/64 ,  G02B21/16 ,  G01N21/05 ,  G02B21/00 ,  G02B27/10 ,  G02F1/11

Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.

公开(公告)号：US12055476B2

公开(公告)日：2024-08-06

申请号：US18214300

申请日：2023-06-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalali ,  Eric D. Diebold ,  Brandon Buckley

IPC: G01N15/1434 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N33/537

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N33/537 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2021/6421 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2201/067

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：US11946851B2

公开(公告)日：2024-04-02

申请号：US18117708

申请日：2023-03-06

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalali ,  Eric D. Diebold ,  Brandon Buckley

IPC: G01N15/14 ,  G01N15/10 ,  G01N15/1434 ,  G01N21/64 ,  G01N33/537

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/64 ,  G01N33/537 ,  G01N2015/1006 ,  G01N2015/1477 ,  G01N2021/6421 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2201/067

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：US20230349810A1

公开(公告)日：2023-11-02

申请号：US18214300

申请日：2023-06-26

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalali ,  Eric D. Diebold ,  Brandon Buckley

IPC: G01N15/14 ,  G01N33/537 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N33/537 ,  G01N21/64 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/6428 ,  G01N2015/1006

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：US20220178813A1

公开(公告)日：2022-06-09

申请号：US17666841

申请日：2022-02-08

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalali ,  Eric D. Diebold ,  Brandon Buckley

IPC: G01N15/14 ,  G01N33/537 ,  G01N21/64

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.

公开(公告)号：US20210255088A1

公开(公告)日：2021-08-19

申请号：US17068573

申请日：2020-10-12

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Bahram Jalali ,  Eric D. Diebold ,  Brandon Buckley

IPC: G01N15/14 ,  G01N33/537 ,  G01N21/64

Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.