公开(公告)号：US20220113671A1

公开(公告)日：2022-04-14

申请号：US17298182

申请日：2019-12-03

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Aniruddha Ray ,  Mustafa Daloglu

IPC: G03H1/00 ,  G02B21/36 ,  G02B21/16

Abstract: A UV holographic imaging device offers a low-cost, portable and robust technique to image and distinguish protein crystals from salt crystals, without the need for any expensive and bulky optical components. This “on-chip” device uses a UV LED and a consumer-grade CMOS image sensor de-capped and interfaced to a processor or microcontroller, the information from the crystal samples, which are placed very close to the sensor active area, is captured in the form of in-line holograms and extracted through digital back-propagation. In these holographic amplitude and/or phase reconstructions, protein crystals appear significantly darker compared to the background due to the strong UV absorption, unlike salt crystals, enabling one to clearly distinguish protein and salt crystals. The on-chip UV holographic microscope serves as a low-cost, sensitive, and robust alternative to conventional lens-based UV-microscopes used in protein crystallography.