公开(公告)号：US12038370B2

公开(公告)日：2024-07-16

申请号：US17621979

申请日：2020-07-02

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Aniruddha Ray ,  Yibo Zhang ,  Dino Di Carlo

IPC: G01N15/1433 ,  B03C1/01 ,  B03C1/02 ,  G01N15/1434 ,  G03H1/00 ,  G06V10/147 ,  G06V10/82 ,  G06V20/69 ,  G01N15/01 ,  G01N15/10

CPC classification number: G01N15/1433 ,  B03C1/01 ,  B03C1/02 ,  G01N15/1434 ,  G03H1/0005 ,  G06V10/147 ,  G06V10/82 ,  G06V20/693 ,  G06V20/698 ,  B03C2201/18 ,  B03C2201/26 ,  G01N15/01 ,  G01N2015/1006 ,  G03H2001/005 ,  G03H2222/12

Abstract: A computational cytometer operates using magnetically modulated lensless speckle imaging, which introduces oscillatory motion to magnetic bead-conjugated rare cells of interest through a periodic magnetic force and uses lensless time-resolved holographic speckle imaging to rapidly detect the target cells in three-dimensions (3D). Detection specificity is further enhanced through a deep learning-based classifier that is based on a densely connected pseudo-3D convolutional neural network (P3D CNN), which automatically detects rare cells of interest based on their spatio-temporal features under a controlled magnetic force. This compact, cost-effective and high-throughput computational cytometer can be used for rare cell detection and quantification in bodily fluids for a variety of biomedical applications.

公开(公告)号：US20220113671A1

公开(公告)日：2022-04-14

申请号：US17298182

申请日：2019-12-03

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Aniruddha Ray ,  Mustafa Daloglu

IPC: G03H1/00 ,  G02B21/36 ,  G02B21/16

Abstract: A UV holographic imaging device offers a low-cost, portable and robust technique to image and distinguish protein crystals from salt crystals, without the need for any expensive and bulky optical components. This “on-chip” device uses a UV LED and a consumer-grade CMOS image sensor de-capped and interfaced to a processor or microcontroller, the information from the crystal samples, which are placed very close to the sensor active area, is captured in the form of in-line holograms and extracted through digital back-propagation. In these holographic amplitude and/or phase reconstructions, protein crystals appear significantly darker compared to the background due to the strong UV absorption, unlike salt crystals, enabling one to clearly distinguish protein and salt crystals. The on-chip UV holographic microscope serves as a low-cost, sensitive, and robust alternative to conventional lens-based UV-microscopes used in protein crystallography.

公开(公告)号：US20220260481A1

公开(公告)日：2022-08-18

申请号：US17621979

申请日：2020-07-02

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Aniruddha Ray ,  Yibo Zhang ,  Dino Di Carlo

IPC: G01N15/14 ,  B03C1/01 ,  B03C1/02 ,  G06V20/69 ,  G06V10/82 ,  G06V10/147 ,  G03H1/00

Abstract: A computational cytometer operates using magnetically modulated lensless speckle imaging, which introduces oscillatory motion to magnetic bead-conjugated rare cells of interest through a periodic magnetic force and uses lensless time-resolved holographic speckle imaging to rapidly detect the target cells in three-dimensions (3D). Detection specificity is further enhanced through a deep learning-based classifier that is based on a densely connected pseudo-3D convolutional neural network (P3D CNN), which automatically detects rare cells of interest based on their spatio-temporal features under a controlled magnetic force. This compact, cost-effective and high-throughput computational cytometer can be used for rare cell detection and quantification in bodily fluids for a variety of biomedical applications.

公开(公告)号：US11460395B2

公开(公告)日：2022-10-04

申请号：US16900838

申请日：2020-06-12

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Aniruddha Ray ,  Hyouarm Joung ,  Derek Tseng ,  Isidro B. Salusky

IPC: B01L3/00 ,  G01N21/01 ,  G01N21/25 ,  G01N33/49 ,  G01N21/78 ,  G01N21/17 ,  G01N21/84

Abstract: A portable colorimetric assay system includes an opto-mechanical reader configured to be detachably mounted to a mobile phone having a camera or other camera-containing portable electronic device. The opto-mechanical reader includes one or more light sources configured to illuminate a test sample holder and control sample holder disposed in the opto-mechanical reader along an optical path aligned with a camera of the mobile phone or other camera-containing portable electronic device. One or more serum separation membranes are disposed in the opto-mechanical reader and define a sample receiving pad configured to receive a blood sample. A moveable serum collection membrane is membrane is also disposed in the reader and is configured to contact the sample receiving pad in a first position and moveable to a second position where the serum collection membrane is disposed inside the test sample holder.