公开(公告)号：US20180052425A1

公开(公告)日：2018-02-22

申请号：US15542794

申请日：2016-01-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Euan McLeod ,  Tevfik Umut Dincer

IPC: G03H1/26 ,  G01B9/021

CPC classification number: G03H1/26 ,  G01B9/021 ,  G01N15/0227 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1463 ,  G01N2001/2826 ,  G01N2015/0038 ,  G01N2015/0046 ,  G01N2015/0053 ,  G01N2015/0233 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2015/1486 ,  G01N2015/1493 ,  G02B21/0008 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/0038 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/261

Abstract: A method for the label-free sizing of small, nanometer-sized objects such as particles includes a hand-held, portable holographic microscope that incorporates vapor condensation of nanolenses and time-resolved lens-free imaging. The portable device is used to generate reconstructed, time-resolved, and automatically-focused phase images of the sample field-of-view. The peak phase value for each object a function of working distance (z2) and condensation time (t) is used to measure object size. The sizing accuracy has been quantified in both monodisperse and heterogeneous particle solutions, achieving an accuracy of +/−11 nm for particles that range from 40 nm up to 500 nm. For larger particles, the technique still works while the accuracy roughly scales with particle size.