公开(公告)号：US20180052425A1

公开(公告)日：2018-02-22

申请号：US15542794

申请日：2016-01-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Euan McLeod ,  Tevfik Umut Dincer

IPC: G03H1/26 ,  G01B9/021

CPC classification number: G03H1/26 ,  G01B9/021 ,  G01N15/0227 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1463 ,  G01N2001/2826 ,  G01N2015/0038 ,  G01N2015/0046 ,  G01N2015/0053 ,  G01N2015/0233 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2015/1486 ,  G01N2015/1493 ,  G02B21/0008 ,  G03H1/0443 ,  G03H1/0866 ,  G03H2001/0038 ,  G03H2001/005 ,  G03H2001/0447 ,  G03H2001/261

Abstract: A method for the label-free sizing of small, nanometer-sized objects such as particles includes a hand-held, portable holographic microscope that incorporates vapor condensation of nanolenses and time-resolved lens-free imaging. The portable device is used to generate reconstructed, time-resolved, and automatically-focused phase images of the sample field-of-view. The peak phase value for each object a function of working distance (z2) and condensation time (t) is used to measure object size. The sizing accuracy has been quantified in both monodisperse and heterogeneous particle solutions, achieving an accuracy of +/−11 nm for particles that range from 40 nm up to 500 nm. For larger particles, the technique still works while the accuracy roughly scales with particle size.

公开(公告)号：US20150153558A1

公开(公告)日：2015-06-04

申请号：US14406199

申请日：2013-06-05

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Onur Mudanyali ,  Euan McLeod

IPC: G02B21/36 ,  G02B21/00 ,  G02B21/14

CPC classification number: G02B21/367 ,  G01B9/04 ,  G01N15/1434 ,  G01N15/1463 ,  G01N2015/1006 ,  G01N2015/144 ,  G02B3/12 ,  G02B21/0004 ,  G02B21/14 ,  G02B21/34 ,  G03H1/0443 ,  G03H1/0465 ,  G03H1/0866 ,  G03H2001/0447 ,  G03H2001/0883 ,  G03H2222/36 ,  G03H2240/56

Abstract: A method of imaging a sample includes depositing a droplet containing the sample on a substrate, the sample having a plurality of particles contained within a fluid. The substrate is then tilted to gravitationally drive the droplet to an edge of the substrate while forming a dispersed monolayer of particles having liquid lenses surrounding the particles. A plurality of lower resolution images of the particles contained on the substrate are obtained, wherein the substrate is interposed between an illumination source and an image sensor, wherein each lower resolution image is obtained at discrete spatial locations. The plurality of lower resolution images of the particles are converted into a higher resolution image. At least one of an amplitude image and a phase image of the particles contained within the sample is then reconstructed. In some embodiments, only a single lower resolution image may be sufficient.

Abstract translation: 成像样品的方法包括将含有样品的液滴沉积在基底上，样品具有包含在流体内的多个颗粒。 然后将衬底倾斜以重力地将液滴驱动到衬底的边缘，同时形成具有围绕颗粒的液体透镜的分散的单层颗粒。 获得包含在基板上的颗粒的多个较低分辨率图像，其中基板被插入在照明源和图像传感器之间，其中在离散的空间位置处获得每个下分辨率图像。 颗粒的多个较低分辨率图像被转换成更高分辨率的图像。 然后重建样品中包含的颗粒的幅度图像和相位图像中的至少一个。 在一些实施例中，仅一个较低分辨率的图像可能是足够的。

公开(公告)号：US10088663B2

公开(公告)日：2018-10-02

申请号：US15154279

申请日：2016-05-13

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Euan McLeod

IPC: G02B21/36 ,  G03H1/04 ,  B05D1/00 ,  C23C14/12 ,  C23C14/22 ,  C23C14/54 ,  G01N15/14 ,  B82Y20/00

Abstract: A method of forming nanolenses for imaging includes providing an optically transparent substrate having a plurality of particles disposed on one side thereof. The optically transparent substrate is located within a chamber containing therein a reservoir holding a liquid solution. The liquid solution is heated to form a vapor within the chamber, wherein the vapor condenses on the substrate to form nanolenses around the plurality of particles. The particles are then imaged using an imaging device. The imaging device may be located in the same device that contains the reservoir or a separate imaging device.

公开(公告)号：US20160334614A1

公开(公告)日：2016-11-17

申请号：US15154279

申请日：2016-05-13

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Aydogan Ozcan ,  Euan McLeod

IPC: G02B21/36 ,  C23C16/44 ,  G03H1/04 ,  G02B1/04 ,  G03H1/00

CPC classification number: G02B21/365 ,  B82Y20/00 ,  C23C14/12 ,  C23C14/223 ,  C23C14/541 ,  G01N15/1468 ,  G02B21/361 ,  G03H1/0443 ,  G03H2001/0447 ,  G03H2001/0452 ,  G03H2001/046 ,  G03H2222/34 ,  G03H2240/56 ,  Y10S977/881 ,  Y10S977/891

Abstract: A method of forming nanolenses for imaging includes providing an optically transparent substrate having a plurality of particles disposed on one side thereof. The optically transparent substrate is located within a chamber containing therein a reservoir holding a liquid solution. The liquid solution is heated to form a vapor within the chamber, wherein the vapor condenses on the substrate to form nanolenses around the plurality of particles. The particles are then imaged using an imaging device. The imaging device may be located in the same device that contains the reservoir or a separate imaging device.

Abstract translation: 一种形成用于成像的纳米技术的方法包括提供具有设置在其一侧上的多个颗粒的光学透明基板。 光学透明基板位于容纳有液体溶液的储存器的腔室内。 液体溶液被加热以在腔室内形成蒸汽，其中蒸汽在衬底上冷凝以在多个颗粒周围形成纳米通道。 然后使用成像装置对颗粒进行成像。 成像装置可以位于包含储存器或单独的成像装置的相同装置中。