公开(公告)号：US20250109377A1

公开(公告)日：2025-04-03

申请号：US18728767

申请日：2023-02-13

Applicant: THE UNIVERSITY OF MASSACHUSETTS

Inventor： Seongkyu Yoon ,  Bingyu Kuang ,  Duc Hoang

IPC: C12N5/00 ,  C12N5/071 ,  G01J3/44 ,  G01N30/02 ,  G01N30/72

Abstract: A method of cell culture includes (i) culturing cells in a cell culture medium, and (ii) maintaining at least one metabolite below an inhibitory concentration in the cell culture medium for the at least one metabolite, wherein the at least one metabolite is aconitic acid (AA), leucinic acid (HICA), cytidine monophosphate (CMP), methylsuccinic acid (MSA), trigonelline (TRI), N-acetylputrescinium (NAP), or a combination thereof, and wherein the enzyme comprises ADH5, BCAT1, CAT, GOT1, HADHB, HOGA1, SLC35A1, or a combination thereof.