公开(公告)号：US11001800B2

公开(公告)日：2021-05-11

申请号：US16598887

申请日：2019-10-10

Applicant: TIANJIN UNIVERSITY

Inventor： Guanyi Chen ,  Daohong Zhang ,  Beibei Yan ,  Zhan Hu ,  Yun Qi

IPC: C12N1/12 ,  C12M1/12 ,  C12M1/00 ,  C12N15/10

Abstract: A microalgae culturing method by using a paper-based culture apparatus, includes: 1) designing the shape of a culture apparatus as a rectangle with circles distributed in its middle; 2) spraying and printing wax on a piece of filter paper; 3) heating such that the wax penetrates through the filter paper to obtain a paper culture apparatus, where a surface of the filter paper on which the wax is sprayed and printed is called a surface A while the other surface thereof is called a surface B; and 4) dropping a first agar aqueous solution onto the circles on the surface B of the paper culture apparatus obtained in the step 3), and dropping a second agar aqueous solution added with microalgae onto the surface A of the paper culture apparatus obtained in the step 3).