公开(公告)号：US11052154B2

公开(公告)日：2021-07-06

申请号：US16349689

申请日：2017-11-13

Applicant: Tokyo University of Science Foundation

Inventor： Etsuko Miyamoto ,  Masaaki Ozawa

IPC: A61K47/54 ,  A61P35/00 ,  A61K38/05

Abstract: A Ras protein degradation inducing molecule that can induce degradation of Ras proteins, and a pharmaceutical composition that contains this Ras protein degradation inducing molecule are provided. The Ras protein degradation inducing molecule is a conjugate of a Ras protein affinity molecule which has affinity to Ras proteins, and a proteolysis-inducing tag which has affinity to protease and does not inhibit proteolysis of proteins by the protease.

公开(公告)号：US11007269B2

公开(公告)日：2021-05-18

申请号：US16349708

申请日：2017-11-13

Applicant: Tokyo University of Science Foundation

Inventor： Etsuko Miyamoto ,  Masaaki Ozawa

IPC: A61K47/54 ,  A61P7/04 ,  A61P35/00 ,  A61K31/167

Abstract: A p53 degradation inducing molecule which can induce degradation of p53 proteins or p53 composites, and a pharmaceutical composition containing said p53 degradation inducing molecule are provided. This p53 degradation inducing molecule is a conjugate of a p53 affinity molecule which has affinity for p53 proteins or p53 composites, and a proteolysis induction tag which has affinity for protease and which does not inhibit proteolysis of proteins by protease.

公开(公告)号：US10976306B2

公开(公告)日：2021-04-13

申请号：US15736089

申请日：2016-06-15

Applicant: Tokyo University of Science Foundation

Inventor： Etsuko Miyamoto ,  Masaaki Ozawa

IPC: G01N33/50 ,  C07D239/49 ,  C07K5/00 ,  C12Q1/02 ,  A61K38/00 ,  C12N9/00 ,  C07K14/47 ,  C12N9/76 ,  C12P21/06

Abstract: Provided are: a protein degradation inducing tag which is a molecule that has affinity with proteases and does not inhibit degradation of a protein by proteases; a protein degradation inducing molecule that is a conjugate of at least one protein degradation inducing tag and at least one protein binding molecule that binds to a protein; and a usage of those.

公开(公告)号：US09513295B2

公开(公告)日：2016-12-06

申请号：US14347707

申请日：2012-09-28

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Etsuko Miyamoto ,  Shigeo Fujimori

IPC: C40B30/04 ,  G01N33/68 ,  C12N15/10 ,  C12Q1/68

CPC classification number: G01N33/6818 ,  C12N15/1034 ,  C12N15/1062 ,  C40B30/04

Abstract: The present invention provides a method for detecting an interaction, which method can solve not only the problem of false negatives but also the problem of false positives. This method is a method for detecting a protein(s) that interact(s) with a target substance(s), the method comprising repeating a (1) transcription step, (2) assignment step, (3) selection step, and (4) amplification step, wherein: (a) in each of a plurality of times of preparation of a cDNA library among the initial preparation of a cDNA library and the round(s) of preparation of a cDNA library in the later amplification step(s), the cDNA library is prepared using a primer(s) having a sequence(s) specific to the time of preparation; (b) the cDNA libraries prepared using the primers having sequences specific to the times of preparation are mixed, and sequences in the cDNA library mixture are determined; (c) the determined sequences are subjected to measurement of the number(s) of molecules encoding the same candidate protein(s) for each time of preparation based on the sequence(s) specific to the time of preparation; and (d) a candidate protein(s) encoded by a molecule(s) that significantly increase(s) as the preparation rounds proceed is/are detected as the protein(s) that interact(s) with the target substance(s).

Abstract translation: 本发明提供一种检测相互作用的方法，该方法不仅可以解决假阴性的问题，而且可以解决假阳性的问题。 该方法是用于检测与目标物质相互作用的蛋白质的方法，该方法包括重复（1）转录步骤，（2）分配步骤，（3）选择步骤和（ 4）扩增步骤，其中：（a）在cDNA文库的初始制备中的cDNA文库的制备中的每一次中，以及后续扩增步骤（s）中cDNA文库的制备循环 ），使用具有与制备时间相关的序列的引物制备cDNA文库; （b）使用具有与制备时间特异性序列的引物制备的cDNA文库混合，并确定cDNA文库混合物中的序列; （c）基于制备时间特有的序列，对每个制备时间对测定的序列进行编码相同候选蛋白质的分子的数目的测定; 和（d）当与制备循环进行时显着增加的分子编码的候选蛋白质被检测为与目标物质相互作用的蛋白质， 。