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公开(公告)号：US20240011889A1
公开(公告)日：2024-01-11
申请号：US18037398
申请日：2021-11-19
Applicant: Texas Tech University System
Inventor： Wei LI , Zhenya DING
CPC classification number: G01N15/1484 , B01L3/502715 , G01N15/1434 , B01L2300/12 , B01L2400/086 , B01L2200/0652 , G01N2015/1006
Abstract: a hyperuniform-structured microchip is disclosed, which is capable of providing viable resolution of CTC subpopulations supporting the ability to determine whether there is a correlation between CTC heterogeneity and tumor progression.
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公开(公告)号：US20250050283A1
公开(公告)日：2025-02-13
申请号：US18930686
申请日：2024-10-29
Applicant: Texas Tech University System
Inventor： Wei LI , Rutwik JOSHI , Gizem LEVENT
Abstract: Hollow glass microspheres (HGMS) with a controlled nanotopographical surface structure (NSHGMS) demonstrate improved isolation and recovery of cells and other biological particles such as bacteria from biological fluid. Such functionalized HGMS are formed by exposing a plurality of hollow glass microspheres to a layer by layer deposition cycle of charged polymeric nanofilms to form a plurality of coated hollow glass microspheres and functionally binding a plurality of biotinylated antibodies to the plurality of coated hollow glass microspheres. Application of these HGMS in related biological particle isolation methods does not require specialized lab equipment or an external power source, and thus, can be used for separation of targeted cells from blood or other fluid in a resource-limited environment.
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公开(公告)号：US20210371813A1
公开(公告)日：2021-12-02
申请号：US17285884
申请日：2019-10-18
Applicant: Texas Tech University System
IPC: C12N5/00
Abstract: Hollow glass microspheres (HGMS) with a controlled nanotopographical surface structure (NSHGMS) demonstrate improved isolation and recovery of cell from biological fluid. NSHGMS can be achieved by applying layer-by-layer (LbL) assembly of negatively charged SiO2 nanoparticles and positively charged poly-L-arginine molecules. Then, a sheathing can be applied to the surface with an enzymatically degradable LbL film made from biotinylated alginate and poly-L-arginine. Further, a cap of anti-EpCAM antibodies and anti-fouling PEG molecules can be applied to the sheathed film covering the microspheres. Compared to smooth-surfaced HGMS, NSHGMS reveals shorter isolation times, enhanced capture efficiency and lower detection limit in, for example, commonly used cancer cell lines. An NSHGMS-based cell isolation method does not require specialized lab equipment or an external power source, and thus, can be used for separation of targeted cells from blood or other body fluid in a resource-limited environment.
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