公开(公告)号：US20150037877A1

公开(公告)日：2015-02-05

申请号：US13956212

申请日：2013-07-31

Applicant: The Arizona Board of Regents on behalf of the University of Arizona

Inventor： Leilei Peng ,  Ming Zhao

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6428 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2201/10 ,  G01N2201/126

Abstract: A fast fluorescence lifetime microscopic system images FRET between multiple labels in live cells and deep tissue, using a quantitative analysis method to reconstruct the molecular machinery behind the multiplexed FRET phenomenon. The system measures fluorescence lifetime, intensity and anisotropy as images of excitation-emission matrices (EEM) in real time and high speed within a single image scan, performs high-resolution deep-penetrating 3D FRET imaging in live samples, and fully analyzes all possible photon pathways of multiplexed FRET. The system provides a way for systematic and dynamic imaging of biochemical networks in cells, tissue and live animals, which will help to understand mechanisms of genetic disorders, cancers, and more.

Abstract translation: 快速荧光寿命显微镜系统在活细胞和深部组织中的多个标记之间的FRET图像，使用定量分析方法重建多重FRET现象背后的分子机制。 该系统在单次图像扫描中以实时和高速的方式测量荧光寿命，强度和各向异性作为激发发射矩阵（EEM）的图像，在活样品中进行高分辨率深穿透3D FRET成像，并全面分析所有可能的 复用FRET的光子通路。 该系统为细胞，组织和活体动物中生物化学网络的系统和动态成像提供了一种途径，有助于了解遗传疾病，癌症等机制。

公开(公告)号：US09217710B2

公开(公告)日：2015-12-22

申请号：US13956212

申请日：2013-07-31

Applicant: The Arizona Board of Regents on behalf of the University of Arizona

Inventor： Leilei Peng ,  Ming Zhao

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6428 ,  G01N2021/6421 ,  G01N2021/6432 ,  G01N2021/6441 ,  G01N2201/10 ,  G01N2201/126

Abstract: A fast fluorescence lifetime microscopic system images FRET between multiple labels in live cells and deep tissue, using a quantitative analysis method to reconstruct the molecular machinery behind the multiplexed FRET phenomenon. The system measures fluorescence lifetime, intensity and anisotropy as images of excitation-emission matrices (EEM) in real time and high speed within a single image scan, performs high-resolution deep-penetrating 3D FRET imaging in live samples, and fully analyzes all possible photon pathways of multiplexed FRET. The system provides a way for systematic and dynamic imaging of biochemical networks in cells, tissue and live animals, which will help to understand mechanisms of genetic disorders, cancers, and more.

Abstract translation: 快速荧光寿命显微镜系统在活细胞和深部组织中的多个标记之间的FRET图像，使用定量分析方法重建多重FRET现象背后的分子机制。 该系统在单次图像扫描中以实时和高速的方式测量荧光寿命，强度和各向异性作为激发发射矩阵（EEM）的图像，在活样品中进行高分辨率深穿透3D FRET成像，并全面分析所有可能的 复用FRET的光子通路。 该系统为细胞，组织和活体动物中生物化学网络的系统和动态成像提供了一种途径，有助于了解遗传疾病，癌症等机制。