公开(公告)号：US09409928B2

公开(公告)日：2016-08-09

申请号：US13649819

申请日：2012-10-11

Applicant: The Hong Kong University of Science and Technology

Inventor： Benzhong Tang ,  Ka Ming Ng ,  Qian Luo ,  Yong Yu ,  Yuning Hong ,  Jianzhao Liu ,  Sijie Chen ,  Wing Yip Lam

IPC: C12Q1/04 ,  C07F7/08 ,  B82Y15/00 ,  G01N21/64

CPC classification number: C07F7/081 ,  B82Y15/00 ,  G01N21/6458 ,  G01N2021/6439 ,  Y10T428/2982

Abstract: Fluorescent bioprobes comprising luminogen formed nanoparticles comprising luminogens with aggregation-induced emission (AIE) properties, which can be used for long-term cell tracking. The luminogens are nonemissive in organic solution but become highly emissive when aggregated in aqueous solution. The fluorescent molecules can readily pass through cell membranes, stain only the cell cytoplasm, and form highly emissive nanoaggregates in aqueous media without any obvious cytoxicity in the living cells. Furthermore, the molecules can be retained inside the cells without noticeable leakage to the outside. Therefore, these AIE-based compounds can be used as selective and cell-compatible fluroescent bioprobes for long-term live cell tracking and imaging.

Abstract translation: 包含发光体形成的纳米颗粒的荧光生物探针包含具有聚集诱发发射（AIE）性质的发光体，其可用于长期细胞跟踪。 发光体在有机溶液中是非发射的，但当在水溶液中聚集时变得高度发射。 荧光分子可以容易地通过细胞膜，仅染色细胞质，并且在水性介质中形成高度发射的纳米聚集体，而在活细胞中没有任何明显的细胞毒性。 此外，分子可以保留在细胞内，而没有明显的泄漏到外部。 因此，这些基于AIE的化合物可用作选择性和细胞相容性的荧光生物探针，用于长期活细胞跟踪和成像。

公开(公告)号：US09618453B2

公开(公告)日：2017-04-11

申请号：US13899070

申请日：2013-05-21

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Ka Ming Ng ,  Qian Luo ,  Yong Yu ,  Yuning Hong ,  Jianzhao Liu ,  Sijie Chen ,  Wing Yip Lam ,  Zhengke Wang ,  Wei Qin ,  Tsz Kin Kwok

IPC: C12Q1/04 ,  G01N21/64 ,  B82Y15/00 ,  G01N1/30 ,  C07F7/08

CPC classification number: G01N21/6486 ,  B82Y15/00 ,  C07F7/0805 ,  C07F7/0816 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439

Abstract: Provided herein are fluorescent bioprobes comprising fluorogens that exhibit aggregation-induced emission (AIE) labeled on biomolecules. The present subject matter relates to a fluorescent bioprobe comprising one or more fluorogen labeled on chitosan. The present subject matter is also directed to methods of preparing the fluorescent bioprobes, methods of labeling and detecting DNA and/or proteins with the fluorescent bioprobe, and methods of cell imaging including live cell tracking.

公开(公告)号：US09518921B2

公开(公告)日：2016-12-13

申请号：US13728150

申请日：2012-12-27

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Wing Yip Lam ,  Jianzhao Liu ,  Faisal Mahtab ,  Yang Liu ,  Yong Yu

IPC: G01N21/64 ,  G01N33/543 ,  G01N33/533 ,  G01N33/58 ,  C07D207/448 ,  C09B69/00 ,  C07D207/46 ,  C07F7/08 ,  C07F7/18 ,  C09B23/14 ,  B82Y15/00

CPC classification number: G01N21/6428 ,  B82Y15/00 ,  C07D207/448 ,  C07D207/46 ,  C07F7/0807 ,  C07F7/1804 ,  C09B23/14 ,  C09B23/148 ,  C09B69/008 ,  G01N33/533 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/582 ,  G01N33/587 ,  Y10S977/773 ,  Y10S977/92

Abstract: Provided herein are magnetic silica fluorescent nanoparticles and fluorescent silica nanoparticles comprising an aggregation induced emission luminogen and magnetite nanoparticles and use of the same as a fluorescent bioprobe for intracellular imaging and a protein carrier. Also provided are processes for preparing and fabricating the same.

Abstract translation: 本文提供了磁性二氧化硅荧光纳米颗粒和包含聚集诱导发射发光体和磁铁矿纳米颗粒的荧光二氧化硅纳米颗粒，并且使用其作为荧光生物探针用于细胞内成像和蛋白质载体。 还提供了制备和制造它们的方法。