公开(公告)号：US20180273582A1

公开(公告)日：2018-09-27

申请号：US15774979

申请日：2016-11-08

Applicant: UNIVERSITY OF NOTRE DAME

Inventor： Basar BILGICER ,  Nur MUSTAFAOGLU ,  Tanyel KIZILTEPE BILGICER

IPC: C07K1/22 ,  C07K16/28 ,  B01J20/26 ,  B01J20/28 ,  B01J20/288 ,  B01J20/32 ,  B01D15/38 ,  B01D71/10

CPC classification number: C07K1/22 ,  B01D15/3823 ,  B01D15/3828 ,  B01D67/0093 ,  B01D69/144 ,  B01D71/10 ,  B01D71/34 ,  B01D71/68 ,  B01J20/265 ,  B01J20/28033 ,  B01J20/2805 ,  B01J20/288 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3246 ,  C07D209/16 ,  C07K16/065 ,  C07K16/2887 ,  C07K2317/24

Abstract: Disclosed are purification systems and methods for providing purified preparations of antibodies from a fluid, particularly a biological fluid comprising or suspected to contain antibody (e.g., blood, serum, plasma, ascites fluid). Reusable and stable synthetic purification columns comprising membranes of a suitable separation matrix material, such as a nylon membrane or regenerated cellulose membrane, having conjugated thereto a small molecule capture ligand, such as a short peptide or protein capable of acting as a ligand for a particular antibody of interest, such as a peptide having a sequence with binding affinity for a nucleotide binding site (NBS) of a selected antibody of interest, are also provided. Methods of preparing the purification columns are also disclosed. Methods for preparing high yield and high purity therapeutic antibody preparations, such as anti-cancer therapeutics, from a biological fluid, are also presented.