公开(公告)号：US20240052313A1

公开(公告)日：2024-02-15

申请号：US17766292

申请日：2020-10-07

Applicant: UNIVERSITY OF UTAH RESEARCH FOUNDATION

Inventor： Teruo Okano ,  Kyungsook Kim ,  Hallie Thorp ,  David W. Grainger ,  Travis Maak

IPC: C12N5/077 ,  A61P19/02

CPC classification number: C12N5/0655 ,  A61P19/02 ,  C12N2506/1353 ,  C12N2533/54 ,  C12N2501/155 ,  C12N2501/60 ,  C12N2501/998 ,  C12N2501/15

Abstract: The disclosure relates to a chondrogenic cell sheet comprising at least two layers of confluent chondrogenically differentiated cells, wherein the cell sheet is prepared from mesenchymal stem cells (MSCs), and wherein the chondrogenically differentiated cells on the basal side of the cell sheet express adhesion molecules. The disclosure also relates to a method of preparing a chondrogenic cell sheet comprising: a) culturing MSCs on temperature responsive cultureware until confluent to form a cell sheet; b) detaching the cell sheet by temperature reduction and allowing the cell sheet to contract, forming a contracted cell sheet; c) contacting the contracted cell sheet with a culture surface; and d) treating the contracted cell sheet on the culture surface with chondrogenic medium and culturing to form a chondrogenic cell sheet. Methods of using the chondrogenic cell sheets to repair cartilage tissue or treat joint disease are also disclosed.