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公开(公告)号：US20250006375A1
公开(公告)日：2025-01-02
申请号：US18820166
申请日：2024-08-29
Applicant: University Health Network
Abstract: This is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides.
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公开(公告)号：US20210156863A1
公开(公告)日：2021-05-27
申请号：US16760522
申请日：2018-11-01
Applicant: UNIVERSITY HEALTH NETWORK , SINAI HEALTH SYSTEM
Inventor： Daniel DINZ DE CARVALHO , Scott Victor BRATMAN , Ankur RAVINARAYANA CHAKRAVARTHY , Rajat SINGHANIA , Justin Matthew BURGENER , Shu Yi SHEN
IPC: G01N33/574 , G16B40/00
Abstract: There is described herein a method of detecting the presence of DNA from cancer cells in a subject comprising: providing a sample of cell-free DNA from a subject; subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; optionally denaturing the sample; capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; sequencing the captured cell-free methylated DNA; comparing the sequences of the captured cell-free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals; identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell-free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals.
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公开(公告)号：US20230212690A1
公开(公告)日：2023-07-06
申请号：US18067661
申请日：2022-12-16
Applicant: UNIVERSITY HEALTH NETWORK
IPC: C12Q1/6886 , G16B30/00
CPC classification number: C12Q1/6886 , G16B30/00 , C12Q2600/154 , C12Q2600/156
Abstract: In an aspect, there is provided a method of detecting the presence of ctDNA from cancer cells in a subject comprising: (a) providing a sample of cell-free DNA from a subject; (b) subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; (c) optionally adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated, then further optionally denaturing the sample; (d) capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; (e) sequencing the captured cell-free methylated DNA; (f) comparing the sequences of the captured cell-free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals; (g) identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell-free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals; wherein in at least one of the capturing step, the comparing step or the identifying step, the subject cell-free methylated DNA is limited to a sub-population according to a fragment length metric.
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