公开(公告)号：US20170059488A1

公开(公告)日：2017-03-02

申请号：US15236510

申请日：2016-08-15

Applicant: University of Geneva

Inventor： Jean-Pierre WOLF ,  Luigi BONACINA ,  Gustavo Alexandre SUBTIL SOUSA

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  A61B5/0071 ,  A61B5/0075 ,  G01J3/427 ,  G01J3/4406 ,  G01N21/636 ,  G01N21/6402 ,  G01N21/6408 ,  G01N2201/06113

Abstract: This invention concerns the field of sample identification, in particular a method and apparatuses for identifying or discriminating biological species from non biological species, both as individual particles and as components of a composition, by pump-probe fluorescence spectroscopy for time-resolved detection or imaging. The method uses the finding that the UV-induced fluorescence of biological molecules is varied, in particular is depleted, by the addition of visible radiation, whereas this does not occur with non-biological organic molecules. The invention discriminates the fluorescence signals of bio and non-bio particles or species using a differential approach, i.e. the comparison. of the total fluorescence recorded with and without additional visible radiation. This allows to discriminate biological particles comprising aromatic amino-acids (AA), like peptides, proteins, bacteria, viruses, pollens, spores, etc., from non-biological particles, like aromatic (AH) or polyaromatic hydrocarbons (PAH), carbonaceous aerosols, soot, etc.

Abstract translation: 本发明涉及样品鉴定领域，特别是用于通过用于时间分辨检测或成像的泵浦探针荧光光谱鉴定或区分来自非生物物种的生物物种的方法和装置，作为单个颗粒和组合物的组分 。 该方法使用通过添加可见光辐射来改变生物分子的紫外线诱导荧光，特别是耗尽，而非生物有机分子不会发生这种发现。 本发明使用差分方法即比较来区分生物和非生物颗粒或物种的荧光信号。 记录有和没有额外的可见光的总荧光。 这允许从非生物颗粒如芳族（AH）或多芳族烃（PAH），含碳的物质（PAH）中区分包含芳族氨基酸（AA），如肽，蛋白质，细菌，病毒，花粉，孢子等的生物颗粒 气溶胶，烟灰等