公开(公告)号：US20230204478A1

公开(公告)日：2023-06-29

申请号：US18166738

申请日：2023-02-09

Applicant: Waters Technologies Corporation

Inventor： Bonnie A. Alden ,  Kenneth Berthelette ,  Jon Finch ,  Donna Osterman ,  Thomas Swann ,  Thomas H. Walter

IPC: G01N1/40 ,  B01D15/42 ,  B01D15/36 ,  B01J39/26 ,  B01J39/05 ,  B01D15/32 ,  B01D15/38 ,  G01N33/92

CPC classification number: G01N1/405 ,  B01D15/426 ,  B01D15/362 ,  B01J39/26 ,  B01J39/05 ,  B01D15/327 ,  B01D15/3847 ,  G01N33/92 ,  G01N2030/062

Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.

公开(公告)号：US11604123B2

公开(公告)日：2023-03-14

申请号：US16230567

申请日：2018-12-21

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Bonnie A. Alden ,  Kenneth Berthelette ,  Jon Finch ,  Donna Osterman ,  Thomas Swann ,  Thomas H. Walter

IPC: G01N1/40 ,  B01D15/38 ,  B01D15/42 ,  B01D15/36 ,  B01J39/26 ,  B01J39/05 ,  B01D15/32 ,  G01N33/92 ,  G01N30/06 ,  G01N30/00

Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.

公开(公告)号：US20220389480A1

公开(公告)日：2022-12-08

申请号：US17831641

申请日：2022-06-03

Applicant: Waters Technologies Corporation

Inventor： Kenneth Berthelette

IPC: C12Q1/68 ,  C12Q1/6806 ,  G01N30/72

Abstract: The present disclosure pertains to methods of separating nucleic acid component compounds from one another. In some embodiments, the methods comprise: (a) loading a sample fluid comprising a plurality of nucleic acid component compounds onto a chromatographic column comprising a zwitterionic stationary phase contained inside the column; (b) flowing a mobile phase through the chromatographic column over a time period thereby forming an eluent in which at least some of the plurality of the nucleic acid component compounds are separated from each other, the mobile phase comprising a polar aprotic solvent, a protic solvent, and a volatile buffer salt, wherein flowing the mobile phase comprises varying a ratio of the protic solvent to the polar aprotic solvent over at least a portion of the time period and varying an ionic strength of the volatile buffer salt over at least a portion of the time period.

公开(公告)号：US20190195758A1

公开(公告)日：2019-06-27

申请号：US16230567

申请日：2018-12-21

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： Bonnie A. Alden ,  Kenneth Berthelette ,  Jon Finch ,  Donna Osterman ,  Thomas Swann ,  Thomas H. Walter

IPC: G01N1/40 ,  B01J39/26 ,  B01D15/36 ,  B01D15/42

CPC classification number: G01N1/405 ,  B01D15/327 ,  B01D15/362 ,  B01D15/3847 ,  B01D15/426 ,  B01J39/05 ,  B01J39/26 ,  G01N2030/009 ,  G01N2030/062

Abstract: In various aspects, the present disclosure pertains to methods of performing a sample enrichment procedure, which comprise: adding a sample fluid that comprises at least one phospholipid and at least one target analyte to a sorbent that comprises a hydrophobic component and a cation exchange component, thereby resulting in sorbent with bound phospholipid and bound target analyte; adding an aqueous solution comprising an acidic compound and a salt; adding an organic solution to the sorbent thereby desorbing at least a portion of the bound phospholipid from the sorbent; and adding an elution solution to the sorbent, thereby desorbing at least a portion of the bound target analyte from the sorbent and forming a solution of the target analyte in the elution solution. In other aspects, the present disclosure pertains to kits, which may be used in conjunction with such methods.