公开(公告)号：US20030175861A1

公开(公告)日：2003-09-18

申请号：US10025145

申请日：2001-12-19

Applicant: Washington State University Research Foundation

Inventor： C.L. Steele ,  Joerg Bohlmann ,  Rodney B. Croteau ,  Michael A. Phillips

IPC: A01H007/00 ,  C07H021/04 ,  C12N009/00 ,  C12N009/10 ,  C12P021/02 ,  C12N005/04

CPC classification number: C11B1/00 ,  A23D9/00 ,  C12N9/88 ,  C12N15/8242 ,  C12N15/8243

Abstract: cDNAs encoding gymnosperm monoterpene synthases have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a monoterpene synthase of the invention. Thus, systems and methods are provided for the recombinant expression of recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts.

Abstract translation: 已经分离并测序了编码裸子植物单萜合酶的cDNA，并确定了相应的氨基酸序列。 提供已经用编码本发明的单萜合酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供用于重组表达重组单萜合酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。