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    Transacylases of the paclitaxel biosynthetic pathway
    1.
    发明申请
    Transacylases of the paclitaxel biosynthetic pathway 审中-公开
    紫杉醇生物合成途径的转酰胺酶

    公开(公告)号：US20030108891A1

    公开(公告)日：2003-06-12

    申请号：US10166984

    申请日：2002-09-18

    Applicant: Washington State University Research Foundation

    Inventor： Rodney B. Croteau ,  Kevin D. Walker ,  Anne Schoendorf ,  Mark R. Wildung

    IPC: C12Q001/68 ,  C07H021/04 ,  C12N009/10 ,  C12P021/02 ,  C12N005/06

    CPC classification number: C12P17/02 ,  C12N9/1029

    Abstract: Transacylase enzymes and the use of such enzymes to produce paclitaxel and related taxoids, as well as intermediates in the paclitaxel biosynthetic pathway, are disclosed. Also disclosed are nucleic acid sequences encoding such transacylase enzymes such as (but without limitation) C-13 phenylpropanoid side chain-CoA acyltransferase and benzoyl-CoA:3null-N-debenzoyl-2null-deoxytaxol N-benzoyltransferase.

    Abstract translation: 公开了转酰胺酶和这种酶在紫杉醇生物合成途径中产生紫杉醇和相关紫杉烷以及中间体的用途。 还公开了编码这样的转酰酶的核酸序列，例如（但不限于）C-13苯丙素侧链-CoA酰基转移酶和苯甲酰辅酶A：3'-N-脱苯甲酰基-2'-脱氧塔考酚N-苯甲酰基转移酶。

    1-deoxy-D-xylulose-5-phosphate reductoisomerases, and methods of use
    3.
    发明申请
    1-deoxy-D-xylulose-5-phosphate reductoisomerases, and methods of use 有权
    1-脱氧-D-木酮糖-5-磷酸还原异构酶及其使用方法

    公开(公告)号：US20010046694A1

    公开(公告)日：2001-11-29

    申请号：US09874562

    申请日：2001-06-04

    Applicant: Washington State University Research Foundation

    Inventor： Rodney B. Croteau ,  Bernd M. Lange

    IPC: C12N009/02 ,  C07H021/04 ,  C12N005/04 ,  C12P021/02

    CPC classification number: C12N9/0006

    Abstract: The present invention relates to isolated DNA sequences which code for the expression of plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein, such as the sequence presented in SEQ ID NO:1 which encodes a 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein from peppermint (Mentha x piperita). Additionally, the present invention relates to isolated plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase protein. In other aspects, the present invention is directed to replicable recombinant cloning vehicles comprising a nucleic acid sequence which codes for a plant 1-deoxy-D-xylulose-5-phosphate reductoisomerase, to modified host cells transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence of the invention.

    Abstract translation: 本发明涉及编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白质的表达的分离的DNA序列，例如编码1-脱氧-D-木酮糖的SEQ ID NO：1所示的序列 -5-薄荷薄荷（Mentha x piperita）的5-磷酸还原异构酶蛋白。 另外，本发明涉及分离的植物1-脱氧-D-木酮糖-5-磷酸还原异构酶蛋白。 在其它方面，本发明涉及可复制的重组克隆载体，其包含编码植物1-脱氧-D-木酮糖-5-磷酸还原异构酶的核酸序列转化，转染，感染和/或注射的修饰的宿主细胞 与本发明的重组克隆载体和/或DNA序列。

    Monoterpene synthases from grand fir (abies grandis)
    4.
    发明申请
    Monoterpene synthases from grand fir (abies grandis) 审中-公开
    来自枞树（Abies grandis）的单萜合酶

    公开(公告)号：US20030175861A1

    公开(公告)日：2003-09-18

    申请号：US10025145

    申请日：2001-12-19

    Applicant: Washington State University Research Foundation

    Inventor： C.L. Steele ,  Joerg Bohlmann ,  Rodney B. Croteau ,  Michael A. Phillips

    IPC: A01H007/00 ,  C07H021/04 ,  C12N009/00 ,  C12N009/10 ,  C12P021/02 ,  C12N005/04

    CPC classification number: C11B1/00 ,  A23D9/00 ,  C12N9/88 ,  C12N15/8242 ,  C12N15/8243

    Abstract: cDNAs encoding gymnosperm monoterpene synthases have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding a monoterpene synthase of the invention. Thus, systems and methods are provided for the recombinant expression of recombinant monoterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts.

    Abstract translation: 已经分离并测序了编码裸子植物单萜合酶的cDNA，并确定了相应的氨基酸序列。 提供已经用编码本发明的单萜合酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供用于重组表达重组单萜合酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。

    Transacylases of the paclitaxel biosynthetic pathway
    6.
    发明申请
    Transacylases of the paclitaxel biosynthetic pathway 审中-公开
    紫杉醇生物合成途径的转酰胺酶

    公开(公告)号：US20040005562A9

    公开(公告)日：2004-01-08

    申请号：US10166984

    申请日：2002-06-10

    Applicant: Washington State University Research Foundation

    Inventor： Rodney B. Croteau ,  Kevin D. Walker ,  Anne Schoendorf ,  Mark R. Wildung

    IPC: C12Q001/68 ,  C07H021/04 ,  C12N009/10 ,  C12P021/02 ,  C12N005/06

    CPC classification number: C12P17/02 ,  C12N9/1029

    Abstract: Transacylase enzymes and the use of such enzymes to produce paclitaxel and related taxoids, as well as intermediates in the paclitaxel biosynthetic pathway, are disclosed. Also disclosed are nucleic acid sequences encoding such transacylase enzymes such as (but without limitation) C-13 phenylpropanoid side chain-CoA acyltransferase and benzoyl-CoA:3null-N-debenzoyl-2null-deoxytaxol N-benzoyltransferase.

    Abstract translation: 公开了转酰胺酶和这种酶在紫杉醇生物合成途径中产生紫杉醇和相关紫杉烷以及中间体的用途。 还公开了编码这样的转酰酶的核酸序列，例如（但不限于）C-13苯丙素侧链-CoA酰基转移酶和苯甲酰辅酶A：3'-N-脱苯甲酰基-2'-脱氧塔考酚N-苯甲酰基转移酶。

    Geranyl diphosphate synthase large subunit, and methods of use
    9.
    发明申请
    Geranyl diphosphate synthase large subunit, and methods of use 审中-公开
    香叶基二磷酸合成酶大亚基，以及使用方法

    公开(公告)号：US20020106772A1

    公开(公告)日：2002-08-08

    申请号：US09934778

    申请日：2001-08-21

    Applicant: Washington State University Research Foundation

    Inventor： Rodney B. Croteau ,  Charles C. Burke

    IPC: C12N009/16 ,  C07H021/04

    CPC classification number: C12N9/1085 ,  A61K48/00 ,  C12N15/8243

    Abstract: A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.

    Abstract translation: 已经分离并测序了从薄荷中编码香叶基二磷酸合酶大亚基的cDNA，并测定了相应的氨基酸序列。 提供可复制的重组克隆载体，其编码香叶基二磷酸合酶大亚基）。 在另一方面，提供已经用编码香叶基二磷酸合成酶大亚基的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 在另一方面，本发明提供了分离的重组香叶基二磷酸合酶蛋白，其包含分离的重组香叶基二磷酸合酶大亚基蛋白和分离的重组香叶基二磷酸合酶小亚基蛋白。 因此，提供了重组表达香叶基二磷酸合成酶的系统和方法。

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