公开(公告)号：US20210223148A1

公开(公告)日：2021-07-22

申请号：US17150793

申请日：2021-01-15

Applicant: Waters Technologies Corporation

Inventor： Paul Rainville ,  Kerri Smith

IPC: G01N1/40 ,  G01N30/72 ,  G01N30/86

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US11959842B2

公开(公告)日：2024-04-16

申请号：US17990029

申请日：2022-11-18

Applicant: Waters Technologies Corporation

Inventor： Paul Rainville ,  Kerri Smith

IPC: G01N30/72 ,  G01N1/40 ,  G01N30/86 ,  G01N30/02

CPC classification number: G01N1/405 ,  G01N30/7233 ,  G01N30/8631 ,  G01N2030/027

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US20240219275A1

公开(公告)日：2024-07-04

申请号：US18604005

申请日：2024-03-13

Applicant: Waters Technologies Corporation

Inventor： Paul Rainville ,  Kerri Smith

IPC: G01N1/40 ,  G01N30/02 ,  G01N30/72 ,  G01N30/86

CPC classification number: G01N1/405 ,  G01N30/7233 ,  G01N30/8631 ,  G01N2030/027

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US11912983B2

公开(公告)日：2024-02-27

申请号：US17112502

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/101 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

公开(公告)号：US20230081572A1

公开(公告)日：2023-03-16

申请号：US17990029

申请日：2022-11-18

Applicant: Waters Technologies Corporation

Inventor： Paul Rainville ,  Kerri Smith

IPC: G01N1/40 ,  G01N30/72 ,  G01N30/86 ,  G01N30/02

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US12216034B2

公开(公告)日：2025-02-04

申请号：US18604005

申请日：2024-03-13

Applicant: Waters Technologies Corporation

Inventor： Paul Rainville ,  Kerri Smith

IPC: G01N30/72 ,  G01N1/40 ,  G01N30/86 ,  G01N30/02

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US20240240170A1

公开(公告)日：2024-07-18

申请号：US18414679

申请日：2024-01-17

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

CPC classification number: C12N15/101 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/315

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.

公开(公告)号：US20230393103A1

公开(公告)日：2023-12-07

申请号：US18206989

申请日：2023-06-07

Applicant: Waters Technologies Corporation

Inventor： Matthew A. Lauber ,  Mathew H. DeLano ,  Scott A. McCall ,  Jonathan Belanger ,  Theodore A. Dourdeville ,  Kerri Smith ,  Paul Rainville ,  Dimple Shah ,  Stephen J. Shiner ,  Catalin Doneanu ,  Michael Donegan

IPC: G01N30/56 ,  B01J20/32 ,  B05D1/00 ,  B01J20/291 ,  C23C16/04 ,  C23C16/30 ,  C23C14/04 ,  C23C14/12 ,  C23C14/24 ,  G01N30/60

CPC classification number: G01N30/56 ,  B01J20/3272 ,  B05D1/60 ,  B01J20/291 ,  C23C16/045 ,  C23C16/30 ,  C23C14/046 ,  C23C14/12 ,  C23C14/24 ,  G01N30/6052 ,  B05D2518/10 ,  G01N30/7266

Abstract: A device for separating analytes is disclosed. The device has a sample injector, sample injection needle, sample reservoir container in communication with the sample injector, chromatography column downstream of the sample injector, and fluid conduits connecting the sample injector and the column. The interior surfaces of the fluid conduits, sample injector, sample reservoir container, and column form a flow path having wetted surfaces. A portion of the wetted surfaces of the flow path are coated with an alkylsilyl coating that is inert to at least one of the analytes. The alkylsilyl coating has the Formula I:






R1, R2, R3, R4, R5, and R6 are each independently selected from (C1-C6)alkoxy, —NH(C1-C6)alkyl, —N((C1-C6)alkyl)2, OH, ORA, and halo. RA represents a point of attachment to the interior surfaces of the fluidic system. At least one of R1, R2, R3, R4, R5, and R6 is ORA. X is (C1-C20)alkyl, —O[(CH2)2O]1-20, -(C1-C10)[NH(CO)NH(C1-C10)]1-20-, or -(C1-C10)[alkylphenyl(C1-C10)alkyl]1-20-.

公开(公告)号：US11530966B2

公开(公告)日：2022-12-20

申请号：US17150793

申请日：2021-01-15

Applicant: Waters Technologies Corporation

Inventor： Paul Rainville ,  Kerri Smith

IPC: G01N30/72 ,  G01N1/40 ,  G01N30/86 ,  G01N30/02

Abstract: The present disclosure discusses a method of separating a sample (e.g., pharmaceutical drug, genotoxic impurity, biomarker, and/or biological metabolite) including coating a metallic flow path of a chromatographic system; injecting the sample into the chromatographic system; flowing the sample through the chromatographic system; separating the sample; and analyzing the separated sample using mass spectroscopy. In some examples, the coating applied to the surfaces defining the flow path is non-binding with respect to the sample—and the separated sample. Consequently, the sample does not bind to the low-binding surface of the coating of the flow path. The applied coating can increase the chromatographic peak area for the sample of the chromatographic system.

公开(公告)号：US20210171932A1

公开(公告)日：2021-06-10

申请号：US17112502

申请日：2020-12-04

Applicant: Waters Technologies Corporation

Inventor： Michael Donegan ,  Martin Gilar ,  Matthew Lauber ,  Scott Mccall ,  Pamela Iraneta ,  Kerri Smith

IPC: C12N15/10 ,  C12N15/113

Abstract: The present technology relates to a method of separating a sample comprising oligonucleotides. The method includes injecting a polyphosphonic acid at a concentration of between about 0.01 M to about 1 M into the sample comprising oligonucleotides. The method also includes flowing the sample and polyphosphonic acid through a liquid chromatography column and separating the oligonucleotides.