公开(公告)号：US11510891B2

公开(公告)日：2022-11-29

申请号：US16955087

申请日：2018-12-17

Applicant: YEDITEPE UNIVERSITESI

Inventor： Fikrettin Sahin ,  Pakize Neslihan Tasli ,  Oguz Kaan Kirbas ,  Ezgi Avsar Apdik ,  Huseyin Apdik

IPC: A61K31/216 ,  A61K9/127

Abstract: A production of a drug, wherein the drug is specific for SH-SY5Y neuroblastoma cancer by loading caffeic acid phenethyl ester (CAPE) to a plurality of microvesicles that a specialized skin cell leaves into a nutrient solution. The objective of the present invention is to provide a cell-specific targeted vesicle development by loading the CAPE to the plurality of microvesicles obtained as a result of culturing and subsequent specialization of a plurality of tissue cells; and to use the CAPE at lower concentrations compared to a state of the art applications to minimize a toxicity of the CAPE to both a plurality of cells other than a plurality of targeted cells and a body.

公开(公告)号：US12138235B2

公开(公告)日：2024-11-12

申请号：US17840700

申请日：2022-06-15

Applicant: YEDITEPE UNIVERSITESI

Inventor： Fikrettin Sahin ,  Pakize Neslihan Tasli ,  Oguz Kaan Kirbas ,  Ezgi Avsar Apdik ,  Huseyin Apdik

IPC: A61K31/216 ,  A61K9/127

Abstract: A method of producing a caffeic acid phenethyl ester loaded microvesicular cancer drug targeting SH-SY5Y neuroblastoma cancer. The method includes seeding a plurality of skin stem cells in a medium; enabling the plurality of skin stem cells in the medium to reach a confluency of 70-80% to be seeded in a plurality of culture plates; preparing a caffeic acid phenethyl ester stock solution by dissolving the caffeic acid phenethyl ester in dimethyl sulfoxide DMSO; performing a microvesicle isolation from specialized skin cells; adding the caffeic acid phenethyl ester into a plurality of microvesicles; and separating the caffeic acid phenethyl ester loaded microvesicles from the caffeic acid phenethyl ester which is free in the solution and not loaded to a microvesicle.