公开(公告)号：US08210728B2

公开(公告)日：2012-07-03

申请号：US12335954

申请日：2008-12-16

Applicant: Ryo Tsutsui ,  Yuichiro Matsuo ,  Yoshihiro Shimada ,  Mitsuo Harada

Inventor： Ryo Tsutsui ,  Yuichiro Matsuo ,  Yoshihiro Shimada ,  Mitsuo Harada

IPC: F21V7/04 ,  F21V9/10 ,  G05D25/02

CPC classification number: G01N21/6428 ,  A61B5/0059 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6484 ,  G01N2201/0624 ,  G01N2201/0627 ,  G02B21/06

Abstract: An illumination apparatus that includes a plurality of LEDs of different center emission wavelengths, a photodetector, a path sharing device for introducing light emitted from the plurality of LEDs into the common optical path, a light-introducing device located on the common optical path to introduce part of the light emitted from the plurality of LEDs passing through the common optical path into the photodetector, a feedback controller controlling turning-on states of the LEDs over preset states in accordance with the amount of light emitted from the plurality of LEDs detected by the photodetector, and an illumination light supplying device located on the common optical path to supply light which is emitted from the plurality of LEDs to pass through the common optical path and is not introduced into the photodetector through the light-introducing device, as illumination light for a cellular analysis.

Abstract translation: 一种照明装置，包括具有不同中心发射波长的多个LED，光电检测器，用于将从多个LED发射的光引入公共光路的路径共享装置，位于公共光路上的光导入装置， 从通过公共光路的多个LED发射的光的一部分光进入光电检测器，反馈控制器根据由多个LED检测到的多个LED发射的光量，控制LED在预设状态下的导通状态 光电检测器和位于公共光路上的照明光供应装置，用于提供从多个LED发射的光，以通过公共光路，并且不通过光导入装置引入到光电检测器中，作为照明光， 细胞分析。

公开(公告)号：US08175370B2

公开(公告)日：2012-05-08

申请号：US12377324

申请日：2007-08-10

Applicant: Kosuke Takagi ,  Yuichiro Matsuo ,  Yoshihiro Shimada

Inventor： Kosuke Takagi ,  Yuichiro Matsuo ,  Yoshihiro Shimada

IPC: G06K9/60

CPC classification number: G01N21/6486 ,  G01N21/6458 ,  G01N2021/6482

Abstract: A sharp image showing a thin projecting part is acquired without using a complex dyeing method, and the analysis accuracy is improved. An automatic cell analyzer (1) comprises: an imaging unit (4) for capturing fluorescence emitted from a cell (S) and acquiring a cell image; an exposure changing section (5) for changing the exposure condition when the imaging unit (4) captures cell images; and a processing section (5) for analyzing the cell (S) on the basis of a plurality of cell images respectively captured under the changed exposure conditions.

Abstract translation: 在不使用复杂染色方法的情况下获取显示薄的突出部分的清晰图像，并且提高分析精度。 一种自动细胞分析仪（1）包括：用于捕获从细胞（S）发射的荧光并获取细胞图像的成像单元（4） 用于当所述成像单元（4）捕获细胞图像时改变所述曝光条件的曝光改变部分（5）; 以及用于基于在改变的曝光条件下分别捕获的多个单元图像来分析单元（S）的处理部分（5）。

公开(公告)号：US07852552B2

公开(公告)日：2010-12-14

申请号：US11970210

申请日：2008-01-07

Applicant: Mitsuo Harada ,  Yoshihiro Shimada

Inventor： Mitsuo Harada ,  Yoshihiro Shimada

IPC: G02B21/00

CPC classification number: G02B21/33 ,  G02B21/248 ,  G02B21/367

Abstract: An observation apparatus for observing an observation object mounted at an observation position on a stage through an immersion objective lens placed below the observation object. The observation apparatus has a liquid pouring device, constructed to be relatively movable with respect to the immersion objective lens, pouring a liquid on a top lens surface of the immersion objective lens from the upper side of the immersion objective lens at a distance from the observation position on the stage; and a position control device automatically adjusting relative positions of a liquid pouring position of the liquid pouring device and the immersion objective lens and automatically adjusting relative positions of the immersion objective lens on which the liquid is poured by the liquid pouring device and a desired observation position on the stage.

Abstract translation: 一种用于通过放置在观察对象下方的浸没物镜观察安装在舞台上的观察位置的观察对象的观察装置。 观察装置具有液体注入装置，其被构造成相对于浸没物镜相对移动，从浸没物镜的上侧距离观察距离处将液体浸入浸没物镜的上侧透镜表面 舞台上的位置 以及位置控制装置，自动调整所述液体注入装置的液体倾倒位置和所述浸没物镜的相对位置，并且自动调节由所述液体注入装置注入所述液体的所述浸没物镜的相对位置和期望的观察位置 在舞台上。

公开(公告)号：US07649686B2

公开(公告)日：2010-01-19

申请号：US12141371

申请日：2008-06-18

Applicant: Mitsuo Harada ,  Yoshihiro Shimada

Inventor： Mitsuo Harada ,  Yoshihiro Shimada

IPC: G02B21/26

CPC classification number: G01N21/6458 ,  G02B21/0088 ,  G02B21/24 ,  G02B21/362

Abstract: A box-type microscope apparatus includes a stage, a microscope, and a housing, which has a fixed housing and a moving housing provided to be openable, closable, and movable with respect to the fixed housing. The box-type microscope apparatus further includes a specimen vessel positioning device for fixing the specimen vessel placed on the stage at a constant position. of the stage and a positioning release device for actuating the specimen vessel positioning means when the moving housing is moved toward a position of a closed state to release a positioning of the specimen vessel performed by the specimen vessel positioning device with respect to the stage when the moving housing is opened.

Abstract translation: 盒式显微镜装置包括台架，显微镜和壳体，其具有设置成可相对于固定壳体可开启，关闭和移动的固定壳体和移动壳体。 盒型显微镜装置还包括用于将放置在台架上的标本容器固定在恒定位置的检体容器定位装置。 以及定位释放装置，用于当移动壳体朝向关闭状态的位置移动时致动样本容器定位装置，以释放由样本容器定位装置执行的样本容器相对于该阶段的定位 移动房屋开放。

公开(公告)号：US4629862A

公开(公告)日：1986-12-16

申请号：US671732

申请日：1984-11-15

Applicant: Hisao Kitagawa ,  Yasuo Inoue ,  Tadafumi Fujihara ,  Itaru Endo ,  Yoshihiro Shimada

Inventor： Hisao Kitagawa ,  Yasuo Inoue ,  Tadafumi Fujihara ,  Itaru Endo ,  Yoshihiro Shimada

IPC: G02B21/30 ,  H05B3/22

CPC classification number: G02B21/30 ,  C12M41/12 ,  C12M41/36

Abstract: A heater for microscopes for heating a sample and maintaining the sample, which is mounted on a platform of the microscope, at a preset temperature during observation of the sample. The heater includes a platform for supporting thereon a sample container and a heater for heating at least part of the platform. A first heater box is fitted, open end down, over the platform and is connected thereto to form a first space which surrounds the sample container. A second heater box is then fitted over the first heater box in such a manner that a second space which surrounds the first heater box is formed. Heater means are provided for heating the second space. Accordingly, the temperatures in the first space and in the second space can be controlled independently to achieve better control over the temperature of the sample which is to be observed and to control condensation which may otherwise form in the sample holding container.

Abstract translation: 用于在样品观察期间加热样品并将安装在显微镜的平台上的样品保持在预设温度的显微镜加热器。 加热器包括用于在其上支撑样品容器的平台和用于加热至少部分平台的加热器。 第一加热箱被安装在平台上并向下开口，并与之相连，形成围绕样品容器的第一空间。 然后将第二加热器盒以这样的方式装配在第一加热器盒上，使得形成围绕第一加热箱的第二空间。 提供加热装置用于加热第二空间。 因此，可以独立地控制第一空间和第二空间中的温度，以便更好地控制待观察的样品的温度并控制另外可能在样品保持容器中形成的冷凝。

公开(公告)号：US09234845B2

公开(公告)日：2016-01-12

申请号：US12090763

申请日：2007-10-19

Applicant: Kenji Kawasaki ,  Yoshihiro Shimada

Inventor： Kenji Kawasaki ,  Yoshihiro Shimada

IPC: G02B21/06 ,  G01N21/64 ,  G02B13/00 ,  G02B21/16 ,  G02B21/00 ,  G02B21/36

CPC classification number: G01N21/6458 ,  G02B13/0095 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/082 ,  G02B21/16 ,  G02B21/361 ,  G02B21/367

Abstract: A microscope includes an objective lens, an imaging lens projecting light passing through the objective lens to form an image of a specimen, an image sensor located at an imaging position where the image of the specimen is formed, an illumination light source, and a reflecting fluorescence illumination optical system including a dichroic mirror introducing light from the illumination light source into an optical to illuminate the specimen with the light. The microscope further includes a relay optical system forming an intermediate image of the specimen between the objective lens and the imaging lens to relay it to the imaging lens. The dichroic mirror of the reflecting fluorescence illumination optical system is located between the relay optical system and a pupil conjugate position that is conjugate with a pupil position of the objective lens, formed between the relay optical system and the imaging lens.

Abstract translation: 显微镜包括物镜，投影透过物镜的光以成像样本的成像透镜，位于形成有检体的图像的摄像位置的图像传感器，照明光源和反射镜 荧光照明光学系统，包括将来自照明光源的光引入光学的二向色镜，以用光照射样本。 显微镜还包括中继光学系统，其形成物镜和成像透镜之间的样本的中间图像，以将其中继到成像透镜。 反射荧光照明光学系统的分色镜位于中继光学系统和与中继光学系统和成像透镜之间形成的物镜的光瞳位置共轭的光瞳共轭位置之间。

公开(公告)号：US08146340B2

公开(公告)日：2012-04-03

申请号：US12680397

申请日：2007-09-27

Applicant: Yoshihiro Shimada ,  Nobuyuki Matsui

Inventor： Yoshihiro Shimada ,  Nobuyuki Matsui

IPC: F16G13/08 ,  F16H7/06 ,  B21L9/04

CPC classification number: F16G13/06 ,  F16G13/04

Abstract: A bush is pressed into a link plate in a turning link rank that turns relatively with a pin. The bush is molded into a seamless cylindrical shape by cold-forging and is coated with a metal carbide layer formed by cementation of chrome, vanadium and other metals and having Vickers hardness of 1300 Hv or more similarly to the pin. Afterwards, the bush is treated by a quenching, tempering or isothermal transformation process. Thereby, wear resistance of the pins and the link plates of a silent (link) chain is improved, productivity is improved and associated costs are reduced.

Abstract translation: 衬套被压入连接板，转向链中的销与针相对转动。 衬套通过冷锻成型为无定形的圆柱形，并且涂覆有通过铬，钒和其它金属的粘结而形成的金属碳化物层，其维氏硬度与销的类似度为1300Hv或更高。 然后，通过淬火，回火或等温转化过程处理衬套。 因此，提高了静音（连杆）链的销和链板的耐磨性，提高了生产率并降低了相关成本。

公开(公告)号：US20100307128A1

公开(公告)日：2010-12-09

申请号：US12680397

申请日：2007-09-27

Applicant: Yoshihiro Shimada ,  Nobuyuki Matsui

Inventor： Yoshihiro Shimada ,  Nobuyuki Matsui

IPC: F16G13/08

CPC classification number: F16G13/06 ,  F16G13/04

Abstract: A bush is pressed into a link plate in a turning link rank that turns relatively with a pin. The bush is molded into a seamless cylindrical shape by cold-forging and is coated with a metal carbide layer formed by cementation of chrome, vanadium and others and having Vickers hardness of 1300 Hv or more similarly to the pin. After that, the bush is treated by a quenching, tempering or isothermal transformation process. Thereby, wear resistance of the pins and the link plates of a silent (link) chain is improved, productivity is improved and the cost is cut.

Abstract translation: 衬套被压入连接板，转向链中的销与针相对转动。 衬套通过冷锻成型为无定形圆柱形，并且涂覆有通过铬，钒等的粘结形成的金属碳化物层，并且与销相似的维氏硬度为1300Hv或更高。 之后，通过淬火，回火或等温转化过程处理衬套。 因此，提高了无声（链）链的销和链板的耐磨性，提高了生产率并降低了成本。

公开(公告)号：US20100182417A1

公开(公告)日：2010-07-22

申请号：US12377257

申请日：2007-08-13

Applicant: Kosuke Takagi ,  Yuichiro Matsuo ,  Yoshihiro Shimada

Inventor： Kosuke Takagi ,  Yuichiro Matsuo ,  Yoshihiro Shimada

IPC: H04N7/18

CPC classification number: G02B21/244 ,  G01N21/6458 ,  H04N5/23212

Abstract: In acquisition of an image of cells, a focal position is accurately set at highly active cells rather than focusing on dead cells. Provided is an automatic focusing apparatus (8) used in a microscope (1) that image captures fluorescence emitted from cells to acquire a cell image, the automatic focusing apparatus (8) including a setting unit (5) that sets a luminance range indicating a region where viable cells exist on the basis of a luminance distribution of the acquired cell image; and a focus-detecting unit that detects a focal position on the basis of a luminance of an image of nuclei of the cells within the luminance range set by the setting unit (5).

Abstract translation: 在获取细胞图像时，将焦点位置精确地设置在高活性细胞上，而不是关注死细胞。 提供了一种在显微镜（1）中使用的自动聚焦装置（8），其中图像捕获从单元发射的荧光以获取单元图像，所述自动对焦装置（8）包括设置单元（5），其设置指示 基于获取的细胞图像的亮度分布存在活细胞的区域; 以及焦点检测单元，其基于由设置单元（5）设置的亮度范围内的单元格的核的图像的亮度来检测焦点位置。

公开(公告)号：US20090046358A1

公开(公告)日：2009-02-19

申请号：US12093409

申请日：2006-11-15

Applicant: Yoshihiro Shimada

Inventor： Yoshihiro Shimada

IPC: G02B21/02

CPC classification number: G02B21/088 ,  G02B21/16

Abstract: The size and production costs of an optical microscope apparatus capable of blocking light or maintaining the specimen environment are reduced. The provided optical microscope apparatus includes a microscope that has a stage for mounting a specimen A, a transmission-illumination optical system, and an detection optical system; and a housing that surrounds the microscope, wherein the housing includes a fixed housing, and a movable housing, wherein, among optical parts constituting the transmission-illumination optical system and the image-forming optical system, at least some optical parts disposed above the stage are movable, and wherein a switching mechanism is provided, the switching mechanism being configured to retract the optical parts away from above the stage when the movable housing is disposed in an open position relative to the fixed housing and to substantially align the optical axes of both optical systems when the movable housing is disposed in a closed position relative to the fixed housing.

Abstract translation: 能够抑制光或维持样品环境的光学显微镜装置的尺寸和生产成本降低。 所提供的光学显微镜装置包括具有用于安装样本A，透射照明光学系统和检测光学系统的台的显微镜; 以及围绕显微镜的壳体，其中壳体包括固定壳体和可移动壳体，其中，在构成透射照明光学系统和图像形成光学系统的光学部件中，至少部分设置在平台上方的光学部件 并且其中设置有切换机构，所述切换机构构造成当所述可移动壳体相对于所述固定壳体设置在打开位置并使所述光学部件的光轴基本对齐时，使所述光学部件远离所述台架的上方 光学系统，当可动壳体相对于固定壳体设置在关闭位置时。