公开(公告)号：US20240425840A1

公开(公告)日：2024-12-26

申请号：US18680688

申请日：2024-05-31

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin ,  Ashley Siegel

IPC: C12N9/78 ,  A01N63/22 ,  A23K10/18 ,  A23K20/189 ,  A61K9/00 ,  A61K35/742 ,  A61K38/00 ,  C07K14/32 ,  C12N9/24 ,  C12N9/42

Abstract: Fusion proteins containing a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member are provided. The fusion proteins further comprise an enzyme having ACC deaminase activity, a phospholipase, a lipase, a xylanase, a xylosidase, a lactonase, a chitosanase, a protease, a glucanase, a phytase, an acid phosphatase, a pectinase, a mannanase, and/or an expansin protein. Also provided are recombinant Bacillus cereus family members that express the fusion proteins, exosporium fragments derived from the recombinant Bacillus cereus family members, and formulations containing the recombinant Bacillus cereus family members or exosporium fragments. Plant seeds treated with the recombinant Bacillus cereus family members, exosporium fragments, or formulations are also provided. The invention further relates to methods for stimulating plant growth and/or promoting plant health using the recombinant Bacillus cereus family members, exosporium fragments, or formulations.

公开(公告)号：US20230134066A1

公开(公告)日：2023-05-04

申请号：US17932994

申请日：2022-09-16

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin

IPC: C05F11/08 ,  C05B15/00 ,  C12N9/78 ,  C05G3/60 ,  A01N63/50 ,  C05F17/20 ,  C05C9/00 ,  C07K14/415 ,  C12N1/20 ,  C12N15/82

Abstract: Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

公开(公告)号：US20220087268A1

公开(公告)日：2022-03-24

申请号：US17540823

申请日：2021-12-02

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Michelle Leslie

IPC: A01N63/50 ,  C07K14/195 ,  C07K14/32 ,  C12N1/20 ,  C12P21/02 ,  A01N63/10 ,  A01N63/22 ,  C07K14/27 ,  A01N63/25 ,  C07K14/21 ,  A01N37/46 ,  C07K14/245

Abstract: Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.

公开(公告)号：US11134681B2

公开(公告)日：2021-10-05

申请号：US16785870

申请日：2020-02-10

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: C12N9/20 ,  C07K14/32 ,  A01N37/46 ,  A01N63/50 ,  A01N63/22 ,  A01N63/10 ,  A01N63/00 ,  C12N9/02 ,  C12N9/16 ,  C12N9/24 ,  A01N37/48 ,  C12N9/42 ,  C12N9/48

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins, formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth and for protecting plants from pathogens by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plant roots.

公开(公告)号：US10428347B2

公开(公告)日：2019-10-01

申请号：US16043477

申请日：2018-07-24

Applicant: Spogen Biotech Inc. ,  Nufarm Limited

Inventor： Brian Thompson

IPC: C12N9/00 ,  C12N15/74 ,  A01N25/12 ,  A01N25/32 ,  C12N15/82 ,  C12N9/04 ,  C08G64/30 ,  C12N9/02

Abstract: Herbicide-detoxifying enzymes, compositions containing one or more of the enzymes, and plant seeds treated with the enzymes are provided. The enzymes can be used in methods for detoxifying auxin herbicides or degrading auxin plant regulators, including in methods for decontaminating surface of an apparatus used in agriculture or pesticide manufacturing, methods for decontaminating water, soil, soilless media, or sludge, and methods for protecting a plant from an auxin herbicide, improving a plant's tolerance to an auxin herbicide, or removing an auxin herbicide from the surface of a plant.

公开(公告)号：US20250136525A1

公开(公告)日：2025-05-01

申请号：US18944720

申请日：2024-11-12

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin

IPC: C05F17/20 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F11/08 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/82

Abstract: Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

公开(公告)号：US20240228394A9

公开(公告)日：2024-07-11

申请号：US18476256

申请日：2023-09-27

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson ,  Jorg Augustin

IPC: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/82

CPC classification number: C05F11/08 ,  A01N63/50 ,  C05B15/00 ,  C05C9/00 ,  C05F17/20 ,  C05G3/60 ,  C07K14/415 ,  C12N1/20 ,  C12N9/78 ,  C12N15/8237 ,  C12N15/8249 ,  C12N15/8279 ,  C12Y302/01 ,  C12Y305/99007 ,  C12Y402/0202 ,  Y02A40/22 ,  Y02P60/21

Abstract: Methods for stimulating plant growth and/or promoting plant health using free enzymes or recombinant microorganisms that overexpress enzymes are provided. Plant seeds coated with free enzymes or recombinant microorganisms that overexpress enzymes are also provided. Compositions comprising a fertilizer and an enzyme or a recombinant microorganism that overexpresses an enzyme are provided. Modified enzymes having ACC deaminase activity, recombinant microorganisms expressing the modified enzymes, plant seeds treated with the modified enzymes or recombinant microorganisms, and methods for stimulating plant growth and/or promoting plant health using the modified enzymes or recombinant microorganisms are also provided.

公开(公告)号：US20240206466A1

公开(公告)日：2024-06-27

申请号：US18392771

申请日：2023-12-21

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Katie Thompson

IPC: A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/10 ,  A01N63/22 ,  A01N63/50 ,  A61K38/16 ,  A61K39/07 ,  C07K14/32 ,  C12N9/02 ,  C12N9/16 ,  C12N9/20 ,  C12N9/24 ,  C12N9/42 ,  C12N9/48

CPC classification number: A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/10 ,  A01N63/22 ,  A01N63/50 ,  A61K38/164 ,  C07K14/32 ,  C12N9/0095 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/48 ,  C12Y118/06001 ,  C12Y301/01003 ,  C12Y302/01004 ,  C12Y304/00 ,  A61K39/07 ,  C07K2319/035 ,  C12Y301/03008

Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

公开(公告)号：US20230152217A1

公开(公告)日：2023-05-18

申请号：US18049889

申请日：2022-10-26

Applicant: Spogen Biotech Inc.

Inventor： Brian M. Thompson

IPC: G01N21/31 ,  G01N21/05 ,  G01J3/02 ,  G01N21/01 ,  G01N21/25 ,  G01N21/80

CPC classification number: G01N21/31 ,  G01N21/05 ,  G01J3/0218 ,  G01N21/01 ,  G01N21/255 ,  G01N21/80 ,  G01N21/3577

Abstract: Systems, devices, and methods for detecting agrochemicals in environments associated with agricultural equipment are described. Certain agrochemicals that are formulated for being detected using the systems, devices, and methods disclosed herein are also described. The devices, systems, and methods disclosed herein are generally configured to use spectral characteristics to detect agrochemicals in an environment associated with agricultural equipment. The spectral characteristics can be analyzed in various ways to provide different types of information about the agrochemicals and/or the environment.

公开(公告)号：US20230016543A1

公开(公告)日：2023-01-19

申请号：US17350746

申请日：2021-06-17

Applicant: Spogen Biotech Inc.

Inventor： Brian Thompson ,  Michelle Leslie

IPC: A01N63/50 ,  A01N37/46 ,  C12P21/02 ,  A01N63/25 ,  A01N63/22 ,  C12N1/20 ,  A01N63/10 ,  C07K14/195 ,  C07K14/21 ,  C07K14/245 ,  C07K14/27 ,  C07K14/32

Abstract: Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.