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    PROBE MEDIUM
    6.
    发明申请
    PROBE MEDIUM 审中-公开
    探索媒介

    公开(公告)号：US20100285997A1

    公开(公告)日：2010-11-11

    申请号：US12779909

    申请日：2010-05-13

    Applicant: Yoshikatsu Okada ,  Makoto Kameyama ,  Kenichi Iwata

    Inventor： Yoshikatsu Okada ,  Makoto Kameyama ,  Kenichi Iwata

    IPC: C40B50/18 ,  C12Q1/68

    CPC classification number: B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

    Abstract: A probe medium for being placed on a substrate comprising a probe which can specifically bond to a target substance, and a substance having a site which can bond to the probe and a substance having a site which can bond to a substrate surface. A process for manufacturing a substrate an immobilized probe comprising the steps of providing a substrate, and providing the substrate with a probe medium comprising a probe which can specifically bond to a target substance and a substance having a site which can bond to the probe and a substance having a site which can bond to a substrate surface. Probe medium comprises a probe which can specifically bond to a target substance, a water-soluble polymer material, and a high boiling solvent. A process for immobilizing a probe comprising the steps of providing a substrate, and providing the substrate with probe medium comprising the probe which can specifically bond to a target substance, a water-soluble polymer material and a high boiling solvent.

    Abstract translation: 用于放置在包含能够特异性地结合到靶物质的探针的基底上的探针介质，以及具有可以结合到探针的位点的物质和具有可以结合到基底表面的位点的物质。 一种用于制造固定化探针的衬底的方法，包括以下步骤：提供衬底，以及向所述衬底提供探针介质，所述探针介质包括可特异性结合靶物质的探针和具有可与所述探针结合的位点的物质和 具有可以结合到基底表面的部位的物质。 探针培养基包括可以特异性结合目标物质的探针，水溶性聚合物材料和高沸点溶剂。 一种用于固定探针的方法，包括提供基底的步骤，以及向基底提供包含能够特异性结合靶物质的探针的探针介质，水溶性聚合物材料和高沸点溶剂。

    Novel method for high-throughput integrated chemical and biochemical reactions
    9.
    发明申请
    Novel method for high-throughput integrated chemical and biochemical reactions 审中-公开
    高通量综合化学和生化反应的新方法

    公开(公告)号：US20090203009A1

    公开(公告)日：2009-08-13

    申请号：US12315385

    申请日：2008-12-03

    Applicant: Marinus Gerardus Johannus Van Beuningen ,  Pieter Jacob Boender

    Inventor： Marinus Gerardus Johannus Van Beuningen ,  Pieter Jacob Boender

    IPC: C12Q1/68 ,  G01N33/53

    CPC classification number: G01N33/551 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00353 ,  B01J2219/00416 ,  B01J2219/00421 ,  B01J2219/00533 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  Y10S436/805 ,  C12Q2565/629

    Abstract: The present invention relates to methods for monitoring in a high through-put fashion a multitude of molecular reaction processes, using a substrate, said substrate comprising a plurality of micro-channels, wherein each micro-channel has inlet and outlet open ends on opposing sides of said substrate, and wherein said micro-channels comprise at least one first reaction component, comprising the steps of: (a) contacting said micro-channels of said solid substrate with a sample, said contacting being via said inlet open ends, wherein said sample comprises an analyte, under conditions that allow said analyte to be specifically retained within said micro-channels by said first reaction component; (b) optionally removing excess of sample via said outlet open ends; (c) contacting the retained analyte with at least one second reaction component, said second reaction component being different from said first reaction component as defined in step (a), under conditions that allow a molecular reaction to take place, said reaction producing a signal; (d) detecting said signal and reading-out reaction results; (e) removing second reaction components via said outlet open ends; (f) repeating steps (c) to (e) at least once, wherein said at least one second reaction component as defined in (c) may be changed in composition; and, final detecting and reading-out of reaction results. The present invention also relates to the uses thereof as well as to microarrays and kits for performing said methods of the invention.

    Abstract translation: 本发明涉及以高通量方式监测多种分子反应过程的方法，使用基底，所述基底包括多个微通道，其中每个微通道在相对侧具有入口和出口开口端 ，并且其中所述微通道包括至少一个第一反应组分，包括以下步骤：（a）使所述固体基质的所述微通道与样品接触，所述接触通过所述入口开口端，其中所述 在允许所述分析物被所述第一反应组分特异性保留在所述微通道内的条件下，样品包含分析物; （b）任选地通过所述出口开口端去除过量的样品; （c）使保留的分析物与至少一个第二反应组分接触，所述第二反应组分与步骤（a）中所定义的所述第一反应组分不同，在允许发生分子反应的条件下，所述反应产生信号 ; （d）检测所述信号并读出反应结果; （e）经由所述出口开口端去除第二反应组分; （f）重复步骤（c）至（e）至少一次，其中如（c）中定义的所述至少一种第二反应组分可以在组成上改变; 并最终检测和阅读反应结果。 本发明还涉及其用途以及用于执行本发明的所述方法的微阵列和试剂盒。

    Protein and peptide arrays
    10.
    发明申请
    Protein and peptide arrays 审中-公开
    蛋白质和肽阵列

    公开(公告)号：US20080242559A1

    公开(公告)日：2008-10-02

    申请号：US11727906

    申请日：2007-03-28

    Applicant: Chad A. Mirkin ,  Seung-Woo Lee ,  Khalid Salaita

    Inventor： Chad A. Mirkin ,  Seung-Woo Lee ,  Khalid Salaita

    IPC: C40B40/10

    CPC classification number: C40B50/18 ,  B01J19/0046 ,  B01J2219/0038 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/00637 ,  B01J2219/00725 ,  B82Y30/00 ,  C40B60/14

    Abstract: Ultrahigh resolution patterning, preferably carried out by DIP PEN™ nanolithographic printing, can be used to construct peptide and protein nanoarrays with nanometer-level dimensions. The peptide and protein nanoarrays, for example, exhibit almost no detectable nonspecific binding of proteins to their passivated portions. This work demonstrates how DIP PEN™ nanolithographic printing can be used in a method to generate high density protein and peptide patterns, which exhibit bioactivity and virtually no non-specific adsorption. It also shows that one can use AFM-based screening procedures to study the reactivity of the features that comprise such nanoarrays. The method encompasses a wide range of protein and peptide structures including, for example, enzymes and antibodies. Features at or below 300 nm can be achieved. In a preferred embodiment, parallel printing with multipen systems are used.

    Abstract translation: 优选通过DIP PEN TM纳米光刻印刷进行的超高分辨率图案可用于构建具有纳米级尺寸的肽和蛋白质纳米阵列。 例如，肽和蛋白质纳米阵列几乎没有显示蛋白质与其钝化部分的可检测的非特异性结合。 这项工作展示了DIP PEN TM纳米光刻印刷如何用于产生高密度蛋白质和肽图案的方法，其显示生物活性并且实际上没有非特异性吸附。 它还显示可以使用基于AFM的筛选程序来研究包含这种纳米阵列的特征的反应性。 该方法包括广泛的蛋白质和肽结构，包括例如酶和抗体。 可以实现300nm以下的特征。 在优选实施例中，使用具有多层系统的平行打印。

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