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    피리도스티그민 정제에서 그 유효성분의 정량분석방법
    8.
    发明授权
    피리도스티그민 정제에서 그 유효성분의 정량분석방법 失效
    吡啶斯的明片中活性成分的定量分析方法

    公开(公告)号：KR1019950006167B1

    公开(公告)日：1995-06-09

    申请号：KR1019910022585

    申请日：1991-12-10

    Applicant: 국방과학연구소

    Inventor： 피택산 ,  조영 ,  석대은 ,  차승희 ,  정윤수

    IPC: G01N30/00

    Abstract: The method for analysing the effective component with a high pressure liquid chromatography(HPLC) includes the steps of: a) particulating the pyridostigmine purification agent; b) extracting the pyridostigmine bromide from it; c) separating the pyridostigmine bromide and the decomposition component and analysing the effective component. When extracting, the methanol is used as the extraction solvent. The mixture of acetonitrile, ice acetic acid, sodium sulfonate hexane is used as the movable solvent so that the decomposition component is analysed simultaneously.

    Abstract translation: 用高压液相色谱（HPLC）分析有效成分的方法包括以下步骤：a）使吡斯的明净化剂微粒化; b）从其中提取吡啶斯的明溴; c）分离吡啶斯坦溴和分解成分，分析有效成分。 萃取时，甲醇用作提取溶剂。 使用乙腈，冰醋酸，磺酸钠己烷的混合物作为可动溶剂，使分解成分同时进行分析。

    수용성 제독제 조성물
    9.
    发明授权
    수용성 제독제 조성물 失效
    水性抗菌组合物

    公开(公告)号：KR1019930009035B1

    公开(公告)日：1993-09-22

    申请号：KR1019900003660

    申请日：1990-03-19

    Applicant: 국방과학연구소

    Inventor： 정윤수 ,  김교국 ,  박훈 ,  박준택 ,  최귀남 ,  이용한

    IPC: A61K33/14 ,  A62D5/00

    Abstract: This relates to an aqueous antidote composition for neutralizing chemical and biological weapons. Antidote composition comprises above 10 wt.% metal salts of dihalo isocyanuric acid, 10-90 wt.% base, 1-10 wt.% iodobenzoate, 0.5-15.wt.% detergent, 0.5-6 wt.% calcium hypochlorite, and 25.-70wt.% organic solvent. Pref. dihaloisocyanurate is selected from sodium dichloroisocyanurate, sodium dibromoisocyanurate, potassium dichloroisocyanurate, potassium dibromoisocyanurate, lithium dichloroisocyanurate, and lithium dibromoisocyanurate. The final antidote solution is made by dissolving the above composition in water and 1-30 wt.% solution is used.

    Abstract translation: 这涉及用于中和化学和生物武器的水性解毒剂组合物。 解毒剂组合物包含10重量％以上的二卤异氰尿酸金属盐，10-90重量％碱，1-10重量％碘苯甲酸盐，0.5-15重量％洗涤剂，0.5-6重量％次氯酸钙和 25.70重量％的有机溶剂。 县。 二卤异氰脲酸酯选自二氯异氰脲酸钠，二溴异氰脲酸钠，二氯异氰尿酸钾，二溴异氰脲酸钾，二氯异氰脲酸锂和二溴异氰脲酸锂。 最终的解毒剂溶液是通过将上述组合物溶解在水中制成的，并且使用1-30重量％的溶液。

    친화성 크로마토그래피법에 의한 소형 전기가오리의 효소인 아세틸콜린에스테라제의 정제방법
    10.
    发明授权
    친화성 크로마토그래피법에 의한 소형 전기가오리의 효소인 아세틸콜린에스테라제의 정제방법 失效
    乙酰胆碱酯酶的纯化方法

    公开(公告)号：KR1019910007852B1

    公开(公告)日：1991-10-02

    申请号：KR1019890001941

    申请日：1989-02-18

    Applicant: 국방과학연구소

    Inventor： 정윤수 ,  석대은 ,  정창희

    IPC: C12N9/16

    Abstract: A process for purifying acetylcholine esterase (I) from electric organ of narke koreanus comprises: (a) cutting the electric organ in 1cm x 1cm and mixing with 0.1M phosphate buffer (pH 7.0); (b) homogenizing the mixt. for 30sec. and centrifuging at 3000g for 30min. to obtain the precipitate; (c) mixing it with 0.1M phosphate buffer (pH 7.0) (II) contg. 0.05M Mgcl2 and 1.4M Nacl and centrifuging at 100000g for 30min. to obtain the crude enzyme soln.; (d) treating the soln. with deoxyribonuclease I at room temp. for 30min.; (e) adfsorbing it to the affinity ligand gel and dissolving with the above buffer (II) to obtain the final product. The obtd. (I) is useful for the detection of agricultural medicines.

    Abstract translation: 从Narke koreanus的电器器官中纯化乙酰胆碱酯酶（I）的方法包括：（a）切割1cm×1cm的电器官并与0.1M磷酸盐缓冲液（pH7.0）混合; （b）均化混合物。 30秒 并在3000g离心30分钟。 得到沉淀物; （c）将其与0.1M磷酸盐缓冲液（pH7.0）（II）混合， 0.05M MgCl 2和1.4M Nacl，并在100000g离心30分钟。 得到粗酶溶胶。 （d）处理溶胶。 与脱氧核糖核酸酶I在室温下。 30分钟 （e）将其吸附到亲和配体凝胶上并用上述缓冲液（II）溶解，得到最终产物。 有限公司 （一）可用于检测农药。

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