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公开(公告)号：KR1020080047676A
公开(公告)日：2008-05-30
申请号：KR1020060117430
申请日：2006-11-27
Applicant: 재단법인서울대학교산학협력재단
Abstract: A mesonephric stem cell line is provided to be differentiated into various cells, treat various diseases or disorders through cell therapy and be used for developing a transgenic animal used for xenotransplantation. A method for preparing a mesonephric stem cell line comprises the steps of: (a) obtaining a mesonephric stem cell from mesonephros of mammal; (b) culturing the mesonephric stem cell onto a feeder cell layer to form a colony; and (c) subculturing the colony to obtain the mesonephric stem cell line, wherein the mammal is human, pig, cattle, sheep, horse, rabbit, goat, mouse, hamster or rat and the feeder cell layer is a fibroblast layer. A mesonephric stem cell line is derived from the mesonephros of the mammal and is characterized in that it show a positive reaction regarding alkaline phosphatase and has the ability of being naturally differentiated into embryonic bodies. Further, the fibroblast is derived from ICR mouse, and a number of the fibroblast is 1.8 to 2.8x10^5 cell/ml.
Abstract translation: 提供中肾干细胞系以分化成各种细胞，通过细胞治疗治疗各种疾病或病症，并用于开发用于异种移植的转基因动物。 制备中肾干细胞系的方法包括以下步骤：（a）从哺乳动物的中肾获得中肾干细胞; （b）将培养的中肾干细胞培养至饲养细胞层以形成菌落; 并且（c）传代培养菌落以获得中肾干细胞系，其中哺乳动物是人，猪，牛，绵羊，马，兔，山羊，小鼠，仓鼠或大鼠，饲养细胞层是成纤维细胞层。 中肾干细胞系衍生自哺乳动物的中肾，其特征在于它显示出对碱性磷酸酶的阳性反应，并具有天然分化成胚体的能力。 此外，成纤维细胞衍生自ICR小鼠，并且许多成纤维细胞是1.8至2.8x10 5细胞/ ml。
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公开(公告)号：KR100838707B1
公开(公告)日：2008-06-16
申请号：KR1020060117430
申请日：2006-11-27
Applicant: 재단법인서울대학교산학협력재단
Abstract: 본 발명은 중신 줄기세포주(mesonephric stem cell line)를 제조하는 방법 및 중신 세포-유래 줄기세포주에 관한 것이다. 본 발명의 방법은 (a) 포유동물의 중신으로부터 중신 세포를 수득하는 단계; (b) 상기 중신 세포를 피더 세포층 상에서 배양하여 콜로니를 형성시키는 단계; 및 (c) 상기 콜로니를 계대 배양하여 중신 줄기세포주를 수득하는 단계를 포함한다. 본 발명의 중신 줄기세포주는 본 발명자들에 의해 최초로 구축된 것이며, 이에 배반포의 내부세포괴와 태아 생식선의 원시생식세포 이외의 새로운 줄기세포 소스를 제시한다. 또한, 본 발명의 중신 줄기세포주는 다양한 세포로 분화될 수 있으며, 세포이식 치료를 통하여 다양한 질병 또는 질환을 치료할 수 있으며, 이종간장기이식(xenotransplantation)에 이용되는 형질전환 동물의 개발에 이용될 수 있다.
중신, 줄기세포, 이종간장기이식, 배양-
公开(公告)号：KR100777229B1
公开(公告)日：2007-11-28
申请号：KR1020060119226
申请日：2006-11-29
Applicant: 재단법인서울대학교산학협력재단
IPC: C12N1/14
Abstract: A method for economically mass-producing zearalenone is provided to obtain large amount of the zearalenone by removing a PKS 12 which synthesizes aurofusarin in the production pathways of the zearalenone. A Gibberella zeae mass-producing zearalenone is deposited as a deposition no. KCCM 10794P. A method for increasing the zearalenone production comprises a step of blocking the aurofusarin biosynthesis pathway of the Gibberella zeae by deleting a PKS12 gene. A method for mass-producing the zearalenone comprises a step of solid-culturing the strain.
Abstract translation: 提供了一种经济地生产玉米赤霉烯酮的方法，通过去除在玉米赤霉烯酮生产途径中合成香叶素的PKS 12来获得大量的玉米赤霉烯酮。 玉米赤霉菌大量生产玉米赤霉烯酮作为沉积物沉积。 KCCM 10794P。 增加玉米赤霉烯酮生产的方法包括通过缺失PKS12基因来阻断玉米赤霉病的aurofusarin生物合成途径。 批量生产玉米赤霉烯酮的方法包括固定培养菌株的步骤。
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公开(公告)号：KR100817429B1
公开(公告)日：2008-03-27
申请号：KR1020060099661
申请日：2006-10-13
Applicant: 재단법인서울대학교산학협력재단
IPC: C12Q1/68
CPC classification number: C12Q1/6809 , C12Q2600/156
Abstract: A DNA polymorphism marker of a lettuce chloroplast is provided to identify lettuce cultivars by finding out the difference between a specific region of a total lettuce chloroplast genome sequence of Lactuca sativa L. cv. Green curled leaf and cultivars analyzed in Japan such as Lactuca sativa L. cv. Cisco or AY865287 and AY865171 deposited in an NCBI, thereby being usefully used for identifying Korean lettuce cultivars and breeding and developing high quality lettuce. A DNA polymorphism marker of a lettuce chloroplast is a sequence of a portion where a 28bp locating between 12064-12210 loci is inserted, the 12064-12210 loci being a region with length variation in a chloroplast genome sequence of the lettuce, and includes a sequence of SEQ ID : NO. 1. A method for identifying lettuce cultivars comprises the steps of: (a) determining a chloroplast genome sequence corresponding to an object lettuce cultivar; and (b) comparing the lettuce chloroplast DNA polymorphism marker with the chloroplast genome sequence corresponding to the object lettuce cultivar. Further, the lettuce is Lactuca sativa L..
Abstract translation: 提供了莴苣叶绿体的DNA多态性标记，通过发现莴苣莴苣叶绿体基因组序列的特定区域之间的差异来鉴定莴苣品种。 在日本分析的绿色卷叶和品种如莴苣Lactuca sativa L.cv。 思科或AY865287和AY865171存放在NCBI中，从而有效地用于识别韩国莴苣品种，并育种和开发高品质的莴苣。 莴苣叶绿体的DNA多态性标记是其中插入位于12064-12210个位点之间的28bp的部分的序列，12064-12210位点是莴苣叶绿体基因组序列中长度变化的区域，并且包括序列 SEQ ID NO： 1.一种鉴定莴苣品种的方法，包括以下步骤：（a）测定对应于莴苣品种的叶绿体基因组序列; 和（b）将莴苣叶绿体DNA多态性标记与对应于莴苣品种的叶绿体基因组序列进行比较。 此外，莴苣是Lactuca sativa L ..
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