公开(公告)号：WO2014054389A1

公开(公告)日：2014-04-10

申请号：PCT/JP2013074399

申请日：2013-09-10

Applicant: KONICA MINOLTA INC

Inventor： OOTANI MAKIKO ,  KAYA TAKATOSHI

IPC: G01N33/543 ,  G01N21/17 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/553 ,  G01N21/648 ,  G01N33/53 ,  G01N33/54373

Abstract: The present invention addresses the problem of providing a surface plasmon resonance (SPR) or surface plasmon excitation enhanced fluorescence spectroscopy (SPFS) immunoassay method, which enables the measurement of whole blood, undergoes little fluctuations in measurement values in the treatment of whole blood, can produce extremely accurate measurement results, and can measure whole blood, serum and plasma in a single apparatus. According to the present invention, the problem can be solved by a surface plasmon resonance (SPR)or surface plasmon excitation enhanced fluorescence spectroscopy (SPFS) immunoassay method utilizing SPR or SPFS, said method comprising: an absorbance measurement step of measuring an absorbance of a sample; a mode setting step of setting a mode corresponding to the absorbance result measured in the absorbance measurement step; and one or multiple steps which are carried out under treatment conditions that are set in accordance with the mode set in the mode setting step.

Abstract translation: 本发明解决提供表面等离子体共振（SPR）或表面等离子体激发增强荧光光谱（SPFS）免疫测定方法的问题，其能够测量全血，在全血治疗中的测量值几乎不发生波动，罐 产生非常精确的测量结果，可以在单个仪器中测量全血，血清和血浆。 根据本发明，可以通过使用SPR或SPFS的表面等离子体共振（SPR）或表面等离子体激发增强荧光光谱（SPFS）免疫测定法来解决问题，所述方法包括：测量吸光度的吸光度测量步骤 样品; 模式设定步骤，设定与吸光度测定步骤中测定的吸光度结果对应的模式; 以及在根据在模式设置步骤中设置的模式设置的处理条件下执行的一个或多个步骤。

公开(公告)号：EP2905617A4

公开(公告)日：2016-08-10

申请号：EP13843487

申请日：2013-09-10

Applicant: KONICA MINOLTA INC

Inventor： OOTANI MAKIKO ,  KAYA TAKATOSHI

IPC: G01N33/543 ,  G01N21/552 ,  G01N21/64 ,  G01N33/53 ,  G01N33/557

CPC classification number: G01N21/6428 ,  G01N21/553 ,  G01N21/648 ,  G01N33/53 ,  G01N33/54373

Abstract: The present invention addresses the problem of providing a SPR (surface plasmon resonance) or SPFS (surface plasmon-field enhanced fluorescence spectroscopy) immunoassay, which enables the measurement of whole blood, undergoes little fluctuations in measurement values, and can measure whole blood, serum and plasma in a single apparatus. The present invention solves the above-mentioned problem by a SPR or SPFS immunoassay, which is an immunoassay utilizing SPR or SPFS, including an absorbance measurement step of measuring an absorbance of a sample, a mode setting step of setting a mode that corresponds to the result of the absorbance measured in the absorbance measurement step, and one or multiple step(s) for which treatment condition (s) has/have been set in accordance with the mode set in the mode setting step.

公开(公告)号：JP2014122888A

公开(公告)日：2014-07-03

申请号：JP2013241876

申请日：2013-11-22

Applicant: Konica Minolta Inc ,  コニカミノルタ株式会社

Inventor： NINOMIYA HIDETAKA ,  MURAYAMA TAKANORI ,  KAYA TAKATOSHI

IPC: G01N21/27 ,  G01N21/45

Abstract: PROBLEM TO BE SOLVED: To provide means capable of analyzing a behavior of water molecules to a thin film made of various materials, from a viewpoint different from the prior arts.SOLUTION: There is provided an analyzing method relating to a behavior of gaseous water molecules with respect to a sample thin film including a step of measuring data (measurement step) relating to a film thickness of the sample thin film by RIfS (reflectometric interference spectroscopy) while continuously or discontinuously changing the humidity of the environment where the sample thin film formed on a surface of a measurement substrate is placed. A RIfS measurement system including humidity adjusting means for continuously changing the humidity of the environment where the sample thin film formed on a surface of a sensor chip is placed is also.provided.

Abstract translation: 要解决的问题：从与现有技术不同的观点，提供能够分析水分子对由各种材料制成的薄膜的行为的手段。解决方案提供了一种与气态水分子的行为有关的分析方法 相对于样品薄膜，包括通过RIfS（反射干涉光谱法）测量与样品薄膜的膜厚度有关的数据（测量步骤）的步骤，同时连续或不连续地改变形成的样品薄膜的环境的湿度 放置在测量基板的表面上。 还提供了包括用于连续地改变形成在传感器芯片的表面上的样品薄膜的环境的湿度的湿度调节装置的RIfS测量系统。

公开(公告)号：EP3130914A4

公开(公告)日：2017-12-06

申请号：EP14889152

申请日：2014-04-08

Applicant: KONICA MINOLTA INC

Inventor： NAKAMURA YUKITO ,  KAYA TAKATOSHI ,  NAGAE KOSUKE ,  NOZAKI AKITOSHI ,  NAGAI FUMIO ,  ISHIKAWA RYOUTA ,  NAMATAME AKIYUKI

IPC: G01N21/64

CPC classification number: G01N21/648 ,  G01J3/0224 ,  G01J3/4406 ,  G01N21/6428 ,  G01N21/6445 ,  G01N33/54373 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2201/0612 ,  G01N2201/0683

Abstract: The present invention pertains to a surface plasmon enhanced fluorescence analysis device and a surface plasmon enhanced fluorescence measurement method which use GC-SPFS and make it possible to detect a substance to be detected with high sensitivity. This surface plasmon enhanced fluorescence measurement device has: a light source for irradiating the diffraction grating of a chip with excited light; a polarizer for removing linearly polarized light from fluorescent light emitted from a fluorescent substance on the diffraction grating; and a photodetector for detecting the linearly polarized light removed by the polarizer.

公开(公告)号：EP3088896A4

公开(公告)日：2017-06-07

申请号：EP13900340

申请日：2013-12-27

Applicant: KONICA MINOLTA INC

Inventor： YAMASHITA KATSUKO ,  KAYA TAKATOSHI ,  IDE YOUICHI ,  KANEKO TOMONORI

IPC: G01N33/68 ,  G01N33/53 ,  G01N33/574

CPC classification number: G01N33/57434 ,  G01N33/6893 ,  G01N2333/42 ,  G01N2333/4725 ,  G01N2800/342

Abstract: An object of the present invention is to provide: a novel method of analyzing diagnostic information, by which prostate cancer and a benign prostate disease can be distinguished from one another with high accuracy; and a diagnostic kit for obtaining the diagnostic information. One embodiment of the method of analyzing diagnostic information according to the present invention is a method of analyzing diagnostic information, which includes: measuring the concentration of a mucin-1 (Tn-MUC1), which is reactive with a lectin that recognizes and binds to an N -acetyl-D-galactosamine †’ serine (threonine) residue, in a blood sample originated from a subject to be diagnosed; comparing the thus measured value with a threshold value; and estimating that a disease affecting the subject to be diagnosed is not prostate cancer but a benign prostate disease when the measured value of the concentration of the mucin-1 is greater than the threshold value, and one embodiment of the diagnostic kit according to the present invention is a diagnostic kit including: an anti-mucin-1 antibody for quantifying Tn-MUC1; and a lectin for detection, which recognizes and binds to an N -acetyl-D-galactosamine †’ serine (threonine) residue.

Abstract translation: 本发明的一个目的是提供：一种分析诊断信息的新方法，通过该方法可以高精度地区分前列腺癌和良性前列腺疾病; 以及用于获得诊断信息的诊断工具。 根据本发明分析诊断信息的方法的一个实施例是一种分析诊断信息的方法，其包括：测量与识别和结合的凝集素反应的粘蛋白-1（Tn-MUC1）的浓度 来源于待诊断受试者的血液样品中的N-乙酰基-D-半乳糖胺†丝氨酸（苏氨酸）残基; 将如此测量的值与阈值进行比较; 并且当粘蛋白-1的浓度的测量值大于阈值时，估计影响要诊断的对象的疾病不是前列腺癌而是良性前列腺疾病，并且根据本发明的诊断试剂盒的一个实施方案 本发明是一种诊断试剂盒，包括：用于定量Tn-MUC1的抗粘蛋白-1抗体; 和用于检测的凝集素，其识别并结合N-乙酰-D-半乳糖胺†丝氨酸（苏氨酸）残基。

公开(公告)号：JP2014025951A

公开(公告)日：2014-02-06

申请号：JP2013223100

申请日：2013-10-28

Applicant: Konica Minolta Inc ,  コニカミノルタ株式会社

Inventor： NAKAMURA YUKITO ,  KAYA TAKATOSHI ,  MATSUO MASAKI ,  NISHIKAWA KUMIKO ,  AOKI YOICHI ,  HIKAGE NAOKI

IPC: G01N21/64

Abstract: PROBLEM TO BE SOLVED: To provide a measurement method of a surface plasmon enhanced fluorescence measurement device that can separate a signal by specific adsorption and improve an S/N ratio even while nonspecific adsorption is caused.SOLUTION: Vibration applying means vibrates an antibody captured in a reaction field where a primary antibody that an analyte specifically reacts with is fixed. One surface of a metal thin film is irradiated with excitation light and an electric field on the other surface side is enhanced to excite a fluorescent material imparted to the antibody. Fluorescence from the fluorescent material is measured and a frequency analysis of an output signal is taken to analyze a sample. In a step of applying the vibration by the vibration applying means, a voltage is applied between the metal thin film and an ITO substrate to vibrate the antibody captured in the reaction field.

Abstract translation: 要解决的问题：提供通过特异吸附分离信号的表面等离子体增强荧光测量装置的测量方法，即使在引起非特异性吸附时也提高S / N比。解决方案：振动施加装置将捕获的抗体振动 其中特异性反应的一级抗体的反应场是固定的。 用激发光照射金属薄膜的一个表面，增强另一个表面侧的电场，激发赋予抗体的荧光材料。 测量荧光材料的荧光，并采用输出信号的频率分析来分析样品。 在通过振动施加装置施加振动的步骤中，在金属薄膜和ITO基板之间施加电压以振动在反应场中捕获的抗体。

公开(公告)号：JP2013181889A

公开(公告)日：2013-09-12

申请号：JP2012046804

申请日：2012-03-02

Applicant: Konica Minolta Inc ,  コニカミノルタ株式会社

Inventor： ISODA TAKETOSHI ,  KAYA TAKATOSHI ,  OTANI MAKIKO

IPC: G01N33/573 ,  G01N21/64 ,  G01N33/543

Abstract: PROBLEM TO BE SOLVED: To provide means for quantifying or detecting CK-MB in a sample with high sensitivity.SOLUTION: An immunoassay targets CK-MB in a sample. The immunoassay includes the steps of: forming a sandwich type immune complex containing a sold phased anti-CK-MB antibody, an anti-CK-MM antibody or anti-CK-BB, CK-MB, a fluorescent labelled anti-CK-MB antibody, and an anti-CK-MM or anti-CK-BB antibody; and measuring fluorescence intensity emitted from a fluorescent body contained in the formed sandwich type immune complex with SPFS (surface plasmon excitation enhanced fluorescence spectrometry).

Abstract translation: 要解决的问题：提供用于定量或检测样品中具有高灵敏度的CK-MB的方法。解决方案：免疫测定目标为样品中的CK-MB。 免疫测定法包括以下步骤：形成含有销售的分阶段抗CK-MB抗体，抗CK-MM抗体或抗-KC-BB，CK-MB，荧光标记抗CK-MB的夹心型免疫复合物 抗体，以及抗CK-MM或抗-CDC-BB抗体; 并用SPFS（表面等离子体激发增强荧光光谱法）测量形成的夹心型免疫复合体中所含荧光体的荧光强度。

公开(公告)号：JP2013145139A

公开(公告)日：2013-07-25

申请号：JP2012004967

申请日：2012-01-13

Applicant: Konica Minolta Inc ,  コニカミノルタ株式会社

Inventor： ISODA TAKETOSHI ,  KAYA TAKATOSHI ,  OTANI MAKIKO

IPC: G01N33/543 ,  G01N21/64 ,  G01N33/53

Abstract: PROBLEM TO BE SOLVED: To provide means capable of highly sensitively quantitatively determining or detecting myoglobin in a sample.SOLUTION: An immunoassay of myoglobin in a sample includes the steps of: forming a sandwich type immune complex including solid-phased anti-myoglobin antibody, myoglobin, and fluorescence-labeled anti-myoglobin antibody; and measuring fluorescence intensity emitted from a fluorescent material included in the formed sandwich type immune complex by an SPFS (Surface Plasmon field-enhanced Fluorescence Spectroscopy).

Abstract translation: 要解决的问题：提供能够高度灵敏地定量测定或检测样品中肌红蛋白的方法。解决方案：样品中肌红蛋白的免疫测定包括以下步骤：形成包含固相的抗肌红蛋白抗体的夹心型免疫复合物， 肌红蛋白和荧光标记的抗肌红蛋白抗体; 并通过SPFS（表面等离子体场增强荧光光谱法）测量由形成的夹心型免疫复合物中包含的荧光材料发射的荧光强度。

公开(公告)号：JP2013145138A

公开(公告)日：2013-07-25

申请号：JP2012004966

申请日：2012-01-13

Applicant: Konica Minolta Inc ,  コニカミノルタ株式会社

Inventor： ISODA TAKETOSHI ,  KAYA TAKATOSHI ,  OTANI MAKIKO ,  HIKAGE NAOKI ,  TSUKAKOSHI MASANORI ,  WADA TAKESHI

IPC: G01N33/53 ,  G01N21/64 ,  G01N33/543

Abstract: PROBLEM TO BE SOLVED: To provide means capable of highly sensitively quantitatively determining or detecting troponin (cTn or the like in a blood specimen) in a sample.SOLUTION: An immunoassay of troponin in a sample includes the steps of: forming in a measurement area of an SPFS sensor chip, a sandwich type immune complex including solid-phased anti-troponin antibody, troponin, and fluorescence-labeled anti-troponin antibody; and measuring fluorescence intensity emitted from a fluorescent material included in the formed sandwich type immune complex by an SPFS (Surface Plasmon field-enhanced Fluorescence Spectroscopy).

Abstract translation: 要解决的问题：提供能够高度灵敏地定量或检测样品中肌钙蛋白（cTn等）的方法。解决方案：样品中肌钙蛋白的免疫测定包括以下步骤：在测量区域中形成 SPFS传感器芯片，包括固相抗肌钙蛋白抗体，肌钙蛋白和荧光标记的抗肌钙蛋白抗体的夹心型免疫复合物; 并通过SPFS（表面等离子体场增强荧光光谱法）测量由形成的夹心型免疫复合物中包含的荧光材料发射的荧光强度。

公开(公告)号：JP2019020406A

公开(公告)日：2019-02-07

申请号：JP2018132474

申请日：2018-07-12

Applicant: KONICA MINOLTA INC

Inventor： OTANI MAKIKO ,  KAYA TAKATOSHI

IPC: G01N33/543 ,  C07K14/47 ,  C07K16/18 ,  C12M1/34 ,  G01N21/05 ,  G01N21/64 ,  G01N35/08

Abstract: 【課題】本発明は、心筋トロポニンIの検出をより迅速かつ正確に、そして高感度に行い、被験者(患者等)から採取するサンプルの少量化(低侵襲性)の達成が可能なセンサーチップおよびこれを用いたSPFS免疫蛍光測定システムを提供することを課題とする。【解決手段】被験者由来の検体中に含まれる心筋トロポニンIと特異的に結合可能な第1の捕捉手段1と、心筋トロポニンIに対する自己抗体と結合可能な第2の捕捉手段2とを備えているSPFS免疫蛍光測定用のセンサーチップ10とし、このセンサーチップ10を用いた免疫診断システムをSPFS免疫蛍光測定システム100とする。【選択図】図1