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公开(公告)号：WO2017177025A9
公开(公告)日：2017-10-12
申请号：PCT/US2017/026386
申请日：2017-04-06
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： LE, Ferrier , RAMAMOORTHI, Kalpith , WILDE, Joyce , FANTIN, Nicole , LANG, Jordan , DUPONT, David , STEVENS, Junko
IPC: C12Q1/68
Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.
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2.发明申请COMPOSITIONS, METHODS, AND KITS FOR SYNTHESIS AND DETECTION OF NUCLEIC ACIDS 审中-公开合成和检测核酸的组合物，方法和试剂盒
公开(公告)号：WO2017177025A1
公开(公告)日：2017-10-12
申请号：PCT/US2017/026386
申请日：2017-04-06
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： LE, Ferrier , RAMAMOORTHI, Kalpith , WILDE, Joyce , FANTIN, Nicole , LANG, Jordan , DUPONT, David , STEVENS, Junko
IPC: C12Q1/68
Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.
Abstract translation: 本文提供了用于合成，检测和/或定量核酸的组合物，方法和试剂盒。 实施方案包含核酸扩增组合物，其包含热稳定DNA聚合酶和改善粗提取物或粗溶解物样品中的核酸靶标的核酸合成，扩增，检测和/或定量的试剂。
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3.发明申请POLYMERIZATION OF NUCLEIC ACIDS USING PROTEINS HAVING LOW ISOELECTRIC POINTS 审中-公开使用具有低电压点的蛋白质的核酸的聚合
公开(公告)号：WO2012170907A2
公开(公告)日：2012-12-13
申请号：PCT/US2012/041687
申请日：2012-06-08
Applicant: LIFE TECHNOLOGIES CORPORATION , WANG, Jonathan , DUPONT, David
Inventor： WANG, Jonathan , DUPONT, David
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12N9/1252 , C12Q1/6846 , C12Y207/07007 , C12Q2527/125
Abstract: This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (PCR).
Abstract translation: 本公开涉及在核酸聚合和/或扩增反应（例如聚合酶链反应）中具有低等电点和/或有限数量（例如零）修饰基团的一种或多种蛋白质（例如，球状蛋白质） （PCR）。
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公开(公告)号：EP3440221A1
公开(公告)日：2019-02-13
申请号：EP17718305.0
申请日：2017-04-06
Applicant: Life Technologies Corporation
Inventor： LE, Ferrier , RAMAMOORTHI, Kalpith , WILDE, Joyce , FANTIN, Nicole , LANG, Jordan , DUPONT, David , STEVENS, Junko
IPC: C12Q1/68
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