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    DNA MARKERS FOR SHRIMP SELECTION
    1.
    发明申请
    DNA MARKERS FOR SHRIMP SELECTION 审中-公开
    用于SHRIMP选择的DNA标记

    公开(公告)号：WO1997021835A2

    公开(公告)日：1997-06-19

    申请号：PCT/US1996019568

    申请日：1996-12-12

    Applicant: WORCESTER POLYTECHNIC INSTITUTE

    Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  BAGSHAW, Joseph, C. ,  BUCKHOLT, Michael, A.

    IPC: C12Q01/68

    CPC classification number: C12Q1/6888 ,  C12Q2600/156

    Abstract: Isolated DNA from Penaeus vannamei was digested with restriction enzymes, restriction fragments of genomic DNA inserted into a plasmid vector and screened for recombinant plasmids containing repeated sequences. Ten of the resulting isolates contained representatives of the same repeated element, a satellite sequence present in one or more blocks of tandemly repeated units. The cloned repeat units range in size from about 100 to 200 base pairs, more typically between about 139 to 188 base pairs. Embedded within each cloned repeat unit are 6 to 15 copies of a tandemly repeated pentanucleotide microsatellite. The genome of P. vannamei contains approximately one million copies of this satellite/microsatellite unit. The sequences are useful as markers for the selection of shrimp having a genetically-transmitted favorable growth characteristic, such as increased reproduction, enhanced growth rate, increased size, disease-resistance, and the ability to grow in colder waters, for improved aquacultured shrimp production. Hybridization of the marker to an isolated Peaneus shrimp mucleic acid molecule can be used to identify species, strains or individual shrimp having the desired characteristics. Once identified, these shrimps can be bred to shrimp having the same or an additional desired characteristic to produce a high quality, genetically superior seedstock or larvae useful for the economic production of farmed shrimp.

    Abstract translation: 用限制性酶，基因组DNA的限制性片段插入到质粒载体中消化来自对虾（Penaeus vannamei）的分离的DNA，并筛选含有重复序列的重组质粒。 所产生的分离物中的10个包含相同重复元件的代表，卫星序列存在于一个或多个串联重复单元的块中。 克隆的重复单元的大小范围为约100至200个碱基对，更典型地在约139至188个碱基对之间。 嵌入每个克隆的重复单元中是6至15个拷贝的串联重复的五核苷酸微卫星。 凡纳滨对虾的基因组包含该卫星/微型卫星单位约100万份。 这些序列可用作选择具有遗传转化的有利生长特征的虾的标记物，例如增加繁殖，增强生长速率，增加的大小，抗病性以及在较冷的水中生长的能力，以改善水产养殖虾生产 。 标记物与分离的豌豆虾核酸分子的杂交可以用于鉴定具有所需特征的物种，菌株或个体虾。 一旦鉴定出来，这些虾可以养殖到具有相同或额外的期望特性的虾，以产生用于经济生产养殖虾的高质量，遗传优良的种子或幼体。

    METHOD OF SELECTING GENETICALLY SUPERIOR SHRIMP
    3.
    发明申请
    METHOD OF SELECTING GENETICALLY SUPERIOR SHRIMP 审中-公开
    选择遗传超高分子量的方法

    公开(公告)号：WO1994001585A1

    公开(公告)日：1994-01-20

    申请号：PCT/US1993006577

    申请日：1993-07-13

    Applicant: WORCESTER POLYTECHNIC INSTITUTE

    Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  BAGSHAW, Joseph, C. ,  BUCKHOLT, Michael, A.

    IPC: C12Q01/68

    CPC classification number: C12Q1/6888 ,  C12Q1/6813 ,  C12Q2600/124 ,  C12Q2600/156

    Abstract: A method for the selection of shrimp having a genetically-transmitted favorable growth characteristic for improved aquacultured shrimp production. A genetic marker for the favorable characteristic is prepared by isolating (Penaeus) shrimp nuclear DNA by extraction with chloroform in the presence of cetyl trimethyl ammonium bromide, digesting the DNA with one or more restriction enzymes, and identifying characteristic restriction fragments. The marker is hybridized to a nuclear nucleic acid molecule isolated from (Penaeus) shrimp for the selection of shrimp having the desired characteristic. The marker can be a labelled probe or a primer for amplification and subsequent detection of a gene that encodes a protein promoting the desired growth characteristic. The selected shrimp can be used to produce a high quality, genetically superior seedstock or larvae useful for the economic production of aquacultured shrimp.

    Abstract translation: 一种用于选择具有遗传转化的有利生长特性的虾的方法，用于改进水产养殖虾生产。 通过在十六烷基三甲基溴化铵存在下用氯仿萃取分离（对虾）虾核DNA，用一种或多种限制酶消化DNA，鉴定特征性限制性片段，制备有利特征的遗传标记。 标记物与从对虾（Penaeus）虾分离的核核酸分子杂交，用于选择具有所需特征的虾。 标记可以是用于扩增和随后检测编码促进所需生长特性的蛋白质的基因的标记探针或引物。 所选择的虾可用于生产高质量，遗传上优质的种子或幼虫，用于经济生产水产养殖虾。

    ARTEMISIA ANNUA TRANSFORMED WITH AGROBACTERIUM RHIZOGENES
    6.
    发明申请
    ARTEMISIA ANNUA TRANSFORMED WITH AGROBACTERIUM RHIZOGENES 审中-公开
    玉米AN A。。。。。。。。。。。。。

    公开(公告)号：WO1994000584A2

    公开(公告)日：1994-01-06

    申请号：PCT/US1993005950

    申请日：1993-06-22

    Applicant: WORCESTER POLYTECHNIC INSTITUTE

    Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  WEATHERS, Pamela, J. ,  CHEETHAM, Ronald, D. ,  DiIORIO, Alexander

    IPC: C12N15/82

    CPC classification number: C07D493/20 ,  C12N5/04 ,  C12N15/8205 ,  C12P17/181

    Abstract: A method of transforming Artemisia annua with Agrobacterium rhizogenes. More particularly, a method for producing an Agrobacterium rhyzogenes hairy root cell culture of Artemisia annua. The method of transformation of A. annua includes the transformation of whole plants with A. rhyzogenes, and also includes transformation of A. annua root cultures with A. rhizogenes. The present invention also relates to transformed A. annua. The present invention also relates to a method for extracting enhanced amounts of a plant secondary metabolite from plant tissue with limited loss of tissue viability by reversibly permeabilizing the tissue membrane is disclosed.

    Abstract translation: 用发根农杆菌转化青蒿的方法。 更具体地说，涉及一种用于生产艾蒿的根癌土壤杆菌毛根细胞培养物的方法。 A. annua的转化方法包括用A. rhyzogenes转化整株植物，还包括A.Anua根培养物与A.nizogenes的转化。 本发明还涉及转化的A. annua。 本发明还涉及一种从植物组织中提取增加量的植物次生代谢物的方法，其中通过可逆地透化组织膜来限制组织活力的丧失。

    METHOD AND DEVICE FOR ELIMINATING ELECTRODE DRIFT
    9.
    发明申请
    METHOD AND DEVICE FOR ELIMINATING ELECTRODE DRIFT 审中-公开
    消除电极脱落的方法和装置

    公开(公告)号：WO1997038625A1

    公开(公告)日：1997-10-23

    申请号：PCT/US1997005700

    申请日：1997-04-08

    Applicant: UNIVERSITY OF MASSACHUSETTS ,  WORCESTER POLYTECHNIC INSTITUTE

    Inventor： UNIVERSITY OF MASSACHUSETTS ,  WORCESTER POLYTECHNIC INSTITUTE ,  DUNN, Raymond, M. ,  HARRINGTON, Robert ,  PEURA, Robert ,  KUN, Stevan

    IPC: A61B05/00

    CPC classification number: A61B5/14542 ,  A61B5/14539 ,  A61B5/1473 ,  A61B5/1495

    Abstract: A pH-measuring method and device (50) for monitoring and then correcting for electrode drift is provided. The device includes a pH-measuring electrode (54) and more than one reference electrodes (52a-52e). During operation, the pH-measuring device is placed in contact with a sample (51). The pH value measured at each electrode pair is due to the electrical potential difference between the pH electrode and the reference electrode. The maximum and minimum pH values are determined, and then the remaining pH values are averaged together to generate an overall average pH. The maximum and minimun pH values are subtracted from the average pH to generate a difference which is then compared to a user defined drift level to determine if a particular electrode is deficient. The pH values from deficient electrodes are not considered when the overall pH of the sample is determined.

    Abstract translation: 提供了用于监测并随后校正电极漂移的pH测量方法和装置（50）。 该装置包括pH测量电极（54）和多于一个的参考电极（52a-52e）。 在操作期间，将pH测量装置与样品（51）接触。 在每个电极对测量的pH值是由于pH电极和参比电极之间的电位差。 确定最大和最小pH值，然后将剩余的pH值平均在一起以产生总体平均pH。 从平均pH中减去最大和最小pH值以产生差异，然后将其与用户定义的漂移水平进行比较，以确定特定电极是否不足。 当确定样品的总体pH时，不考虑来自缺陷电极的pH值。

    PREPARATION OF SOLID STATE MATERIALS
    10.
    发明申请
    PREPARATION OF SOLID STATE MATERIALS 审中-公开
    固态材料的制备

    公开(公告)号：WO1994004263A1

    公开(公告)日：1994-03-03

    申请号：PCT/US1993007752

    申请日：1993-08-18

    Applicant: WORCESTER POLYTECHNIC INSTITUTE

    Inventor： WORCESTER POLYTECHNIC INSTITUTE ,  MOSER, William, R.

    IPC: B01J19/00

    CPC classification number: B01J37/031 ,  Y10S502/523

    Abstract: A process for the preparation of solid state materials such as catalysts, electrolytes, piezo electric materials and superconductors is disclosed. The process produces materials with high phase purity. Novel solid state materials having high phase purity are also disclosed.

    Abstract translation: 公开了一种制备固体材料如催化剂，电解质，压电材料和超导体的方法。 该方法生产具有高相纯度的材料。 还公开了具有高相纯度的新型固态材料。

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