公开(公告)号：EP1203099A2

公开(公告)日：2002-05-08

申请号：EP00952450.5

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： HADD, Andy ,  JOVANOVICH, Stevan

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01J2219/00286 ,  B01J2219/00369 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2300/1844 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/143333 ,  Y10T436/2575 ,  C12Q2565/518 ,  C12Q2535/101 ,  C12Q2527/125

Abstract: Methods for preparing nanoscale reactions using nucleic acids are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Alternatively, the saturably bound nucleic acid is eluted, dispensing a metered amount of nucleic acid for subsequent reaction in a separate chamber. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput nucleic acid sequencing reactions are also provided.

公开(公告)号：EP1200191A1

公开(公告)日：2002-05-02

申请号：EP00952424.0

申请日：2000-08-02

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： JOVANOVICH, Stevan, B. ,  ROACH, David, J. ,  HADD, Andrew, G. ,  HELLMAN, Bo, E., R.

IPC: B01L7/00 ,  B01L3/00

CPC classification number: C12Q1/6869 ,  B01J2219/00286 ,  B01J2219/00369 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00691 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L7/52 ,  B01L2300/0838 ,  B01L2300/1844 ,  B01L2400/0409 ,  B01L2400/0487 ,  C12Q1/6806 ,  C12Q1/6834 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/809 ,  Y10T436/113332 ,  Y10T436/143333 ,  Y10T436/2575 ,  C12Q2565/518 ,  C12Q2535/101 ,  C12Q2527/125

Abstract: An automated system utilizes an array of nanoscale capillary-dimension reaction chambers (12). The ends of the chambers are temporarily sealed with deformable membranes (264a, 264b) and the reactions effected by incubation or temperature cycling. The reaction containers may be filled by capillary action and dispensed by air displacement, centrifugal force or other means. Reaction mixtures may be assembled by using the reaction chambers (12) to meter reaction components that are combined on a substrate. Alternatively, a first reaction component may be immobilized on the interior surface of the reaction container and a second mixture component pumped of drawn into the container to form a final reaction mixture.

公开(公告)号：EP1051390A1

公开(公告)日：2000-11-15

申请号：EP99903154.5

申请日：1999-01-19

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： DOLNIK, Vladislav ,  CHIARI, Marcella

IPC: C07C231/02 ,  C07C233/16

CPC classification number: C07C233/20 ,  C08F20/58 ,  G01N27/44752

Abstract: The present invention provides a new class of linear and cross-linked polymers and copolymers of acrylamidomonomers or methacrylamides bearing two hydroxyethyl residues. The invention describes a method to produce this new polymer. Additionally the invention describes a method of using the new compound as a capillary wall coating for the suppression of electroosmotic flow.

公开(公告)号：EP0724719A4

公开(公告)日：1998-12-09

申请号：EP95924012

申请日：1995-06-20

Applicant: MOLECULAR DYNAMICS

Inventor： HUETON IAIN ,  VAN GELDER EZRA

IPC: G01N21/64 ,  G01N27/447 ,  G01N21/61

CPC classification number: G01N21/6452 ,  G01N27/44721 ,  G01N2021/6417 ,  G01N2201/101

Abstract: A high-speed fluorescence scanner for scanning a sample at equal angles has most of its optical components, including a light beam source (11), a detector (19), filters (18), lenses (17), and reflectors (15-16), in a fixed position (24), removed from the scan head (22). The lightweight scan head contains a single reflector (13) and lens (12) combination which is reciprocated rapidly along one axis to lengthen and shorten a region of the path of a collimated excitation beam (30) and to form a scan line (25-26) on a sample. The fluorescence emission is gathered by the lens (12) of the scan head and directed back, generally along the optical path of the excitation beam, to a detector. Another embodiment places the light source (43), in miniature form, directly on the scan head. The sample may be translated in an axis orthogonal to the scan line to stimulate fluorescent emission from two-dimensional portion of the sample.

Abstract translation: 用于以相等角度扫描样品的高速荧光扫描仪具有其大部分光学部件，包括光束源（11），检测器（19），滤光片（18），透镜（17）和反射镜（15- 从固定位置（24）移开，从扫描头（22）上移除。 轻便的扫描头包含单个反射器（13）和透镜（12）组合，其沿一个轴线快速往复运动，以加长和缩短准直激励光束（30）的路径区域并形成扫描线（25- 26）在样品上。 荧光发射由扫描头的透镜（12）收集并通常沿着激发光束的光路返回到检测器。 另一个实施例将微型形式的光源（43）直接放置在扫描头上。 样品可以在与扫描线正交的轴上平移以激发来自样品的二维部分的荧光发射。

公开(公告)号：EP0746865A1

公开(公告)日：1996-12-11

申请号：EP95937560.0

申请日：1995-10-20

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： KAIN, Robert, C.

IPC: G01N21 ,  G02B21

CPC classification number: G02B21/002 ,  G02B21/16

Abstract: Fluorescence imaging system includes objective (21) which is achromatic and has an external entrance pupil (29), serves as a condenser for the system, and positioned above sample (23) in close proximity to one another. Laser (18) directs collimated light (19) to scan device (20) located at entrance pupil (29). Scan device (20) reflects, refracts, or diffracts light through objective (21) to illuminate a spot (22) on the sample's surface, and illuminates a line or an area on the sample surface by varying the angle of laser light into objective (21). Sample (23) emits fluorescent light (24) in response to the illumination. The fluorescent light (24) is collected by objective (21) and passes through the system along the path of the illumination light. Wavelength-discriminating dichroic filter (25), placed along the optical axis between laser (18) and objective (21), directs fluorescent light (24) onto photodetector (26) to produce a signal representing the sample surface emitting the fluorescent light. Display (37) displays the digitized data in a raster format.

Abstract translation: 荧光成像系统包括无色的物镜（21），其具有外部入射光瞳（29），用作系统的聚光器，并且位于样品（23）上方彼此靠近。 激光器（18）将准直光（19）引导到位于入瞳（29）处的扫描装置（20）。 扫描装置（20）通过物镜（21）反射，折射或衍射光线以照射样品表面上的斑点（22），并且通过将激光角度改变成物镜来照亮样品表面上的线或区域 21）。 样品（23）响应于照射而发射荧光（24）。 荧光灯（24）由物镜（21）收集并沿着照明光的路径通过系统。 沿着激光器（18）和物镜（21）之间的光轴放置的波长识别二向色滤光器（25）将荧光（24）引导到光探测器（26）上以产生表示发射荧光的样品表面的信号。 显示（37）以光栅格式显示数字化数据。

公开(公告)号：EP0670728A1

公开(公告)日：1995-09-13

申请号：EP94901482.0

申请日：1993-11-12

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： SCHLESINGER, David

IPC: A23L3 ,  A61K8 ,  A61K38 ,  A61K47 ,  A61Q15 ,  C07K7 ,  C07K14

CPC classification number: C07K14/001 ,  A23L3/3463 ,  A23L3/3526 ,  A61K8/64 ,  A61K47/64 ,  A61K47/645 ,  A61K2800/57 ,  A61Q15/00 ,  C07K7/02

Abstract: Composition, methods of synthesis and uses of a peptide-based moiety for oral drug delivery, having the general formula: [X-A]2$(1,2)$ - [B]2$(1,2)$-1 - C, wherein X is an amino acid blocking group; A is selected from the group consisting of at least one amino acid; B is selected from the group consisting of lysine, ornithine and combinations thereof; C is selected from the group consisting of a free carboxyl group, or a protected carboxamide (CONH2); and n is the number of cycles of addition of B, including the addition of various xenobiotics and endogenous drugs.

公开(公告)号：EP2537661B1

公开(公告)日：2016-07-13

申请号：EP12164727.5

申请日：2012-04-19

Applicant: Coretech System Co., Ltd. ,  Molecular Dynamics Technology Co., Ltd.

Inventor： Tseng, Huan Chang ,  Chang, Rong Yeu ,  Hsu, Chia Hsiang

IPC: B29C45/76 ,  B29C45/00 ,  B29K105/06 ,  B29K105/18 ,  G06F17/50

CPC classification number: B29C45/0005 ,  B29C45/7693 ,  B29K2105/06 ,  G06F17/5009

公开(公告)号：EP0746865B1

公开(公告)日：2003-03-26

申请号：EP95937560.1

申请日：1995-10-20

Applicant: MOLECULAR DYNAMICS, INC.

Inventor： KAIN, Robert, C.

IPC: H01J3/14 ,  G01N21/64 ,  G02B21/00 ,  G02B21/16

CPC classification number: G02B21/002 ,  G02B21/16

Abstract: Fluorescence imaging system includes objective (21) which is achromatic and has an external entrance pupil (29), serves as a condenser for the system, and positioned above sample (23) in close proximity to one another. Laser (18) directs collimated light (19) to scan device (20) located at entrance pupil (29). Scan device (20) reflects, refracts, or diffracts light through objective (21) to illuminate a spot (22) on the sample's surface, and illuminates a line or an area on the sample surface by varying the angle of laser light into objective (21). Sample (23) emits fluorescent light (24) in response to the illumination. The fluorescent light (24) is collected by objective (21) and passes through the system along the path of the illumination light. Wavelength-discriminating dichroic filter (25), placed along the optical axis between laser (18) and objective (21), directs fluorescent light (24) onto photodetector (26) to produce a signal representing the sample surface emitting the fluorescent light. Display (37) displays the digitized data in a raster format.

公开(公告)号：EP1194770A1

公开(公告)日：2002-04-10

申请号：EP00945324.2

申请日：2000-07-11

Applicant: MOLECULAR DYNAMICS, INC. ,  Peponnet, Christine

Inventor： PEPONNET, Christine ,  MANSFIELD, Elaine, S. ,  BASHKIN, John, S. ,  KAUTZER, Curtis, R.

IPC: G01N27/447

CPC classification number: G01N27/44743 ,  B01J2219/00315 ,  B01J2219/00587 ,  B01J2219/00707 ,  C40B60/14 ,  G01N27/44782

Abstract: The apparatus and method of the present invention disclose a system in which multiple injections may be made into a capillary array. The injections are spaced in time with each injection followed by an interval of electrophoresis. Once all samples are loaded into the capillaries, continuous electrophoresis and detection is used to separate and detect target compounds within the sample. The interval between injections is matched to the target compound migration rate to be sufficient to allow the target compounds to be detectably separated when the compounds reach the detector.

公开(公告)号：EP1129098A2

公开(公告)日：2001-09-05

申请号：EP99967097.9

申请日：1999-11-12

Applicant: Molecular Dynamics, Inc.

Inventor： MEADE, Thomas, J. ,  YU, Changjun

IPC: C07H19/06 ,  C07H19/16 ,  C07H21/00 ,  C07H23/00

CPC classification number: C07H19/06

Abstract: The invention is directed to novel methods of making nucleosides modified with signalling moieties and polydentate ligands, particularly for use in chelating transition metal complexes to form signalling moieties such as electron transfer moieties and fluorophores.