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公开(公告)号：KR101327248B1
公开(公告)日：2013-11-13
申请号：KR1020110074288
申请日：2011-07-26
Applicant: 고려대학교 산학협력단
CPC classification number: C12N1/20
Abstract: 본 발명은 3,6-안하이드로--갈락토오스를 대사하는 신규한 해양미생물 및 이 균주의 용도에 관한 것으로 더욱 상세하게는 3,6-안하이드로-L-갈락토오스를 대사하는 신규한 미생물인
Vibio
sp . EJY3 및 이를 이용한 3,6-안하이드로-L-갈락토오스의 대사 경로 규명 및 대사물의 이용에 관한 것이다.-
公开(公告)号：KR101286211B1
公开(公告)日：2013-07-15
申请号：KR1020120015703
申请日：2012-02-16
Applicant: 고려대학교 산학협력단
IPC: H01L33/22
CPC classification number: H01L33/22 , H01L33/405 , H01L33/42 , H01L33/60 , H01L2933/0016 , H01L2933/0058 , H01L2933/0091
Abstract: PURPOSE: A method for fabricating a light emitting device and a light emitting device fabricated by using the same are provided to improve light extraction efficiency by reducing the total reflection of the light generated from an active layer. CONSTITUTION: A light emitting structure including a first conductive semiconductor layer, an active layer, and a second conductive semiconductor layer is prepared (S10). A light extraction layer having a recess pattern is formed on the upper part of the second conductive semiconductor layer (S20). The light emitting structure is dipped into a solution including nanomaterials (S30). The nanomaterials are adsorbed onto the light extraction layer (S40). An electrode is formed on the light extraction layer (S50). [Reference numerals] (S10) Light emitting structure is prepared; (S20) Light extraction layer is formed; (S30) Dipping; (S40) Adsorption; (S50) Electrode is formed
Abstract translation: 目的：提供一种制造发光器件的方法和使用该方法制造的发光器件，以通过减少由有源层产生的光的全反射来提高光提取效率。 构成：制备包括第一导电半导体层，有源层和第二导电半导体层的发光结构（S10）。 在第二导电半导体层的上部形成具有凹凸图案的光提取层（S20）。 将发光结构浸入包括纳米材料的溶液中（S30）。 纳米材料被吸附到光提取层上（S40）。 在光提取层上形成电极（S50）。 （附图标记）（S10）准备发光结构体; （S20）形成光提取层; （S30）浸渍; （S40）吸附; （S50）形成电极
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公开(公告)号：KR1020120116612A
公开(公告)日：2012-10-23
申请号：KR1020110034150
申请日：2011-04-13
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A novel alginase and a decomposition method thereof are provided to enhance decomposition activity of alginic acid and to produce more polymerized alginic acid products. CONSTITUTION: A novel alginase has an amino acid sequence described in the sequence number 1(SEQ ID NO:1). The enzyme is originated from Saccharophagus degradans. The gene coding the alginase has the base sequence described in the sequence number 2. The composition for the alginic acid decomposition includes alginase or a gene coding the same. The alginic acid is oligo alginic acid. The alginase produces oligo alginic acid products by processing the alginase to the alginic acid or oligo alginic acid. The polymerization degree of the oligo alginic acid product is 2-5. The reaction is operated in the pH of 6-10, at 30-60 deg. Celsius or under the existence of Na+ cation presence.
Abstract translation: 目的：提供一种新颖的藻酸酶及其分解方法，以提高藻酸的分解活性，并产生更多的聚合藻酸产品。 构成：新型藻酸酶具有序列号1（SEQ ID NO：1）中描述的氨基酸序列。 该酶源自酿酒酵母（Saccharophagus degradationans）。 编码藻酸酶的基因具有序列号2中描述的碱基序列。藻酸分解的组合物包括藻酸酶或编码它的基因。 藻酸是寡聚藻酸。 藻酸酶通过将藻酸酶加工成藻酸或寡聚藻酸来生产低聚藻酸产物。 寡聚藻酸产物的聚合度为2-5。 反应在6-10℃，30-60℃下进行。 摄氏度或存在Na +阳离子存在。
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公开(公告)号：KR1020120031750A
公开(公告)日：2012-04-04
申请号：KR1020100093316
申请日：2010-09-27
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A novel alpha-neoagarobios hydrolase and three-dimensional structure thereof are provided to save pretreatment costs and to improve yield. CONSTITUTION: An alpha-neoagarobios hydrolase contains an aspartate 52 residue, an aspartate 207 residue, and a glutamate 265 residue. The enzyme further contains an arginine 235 residue, a serine 358 residue, and a tyrosine 359 residue. A method for crystallizing the alpha-neoagarobios hydrolase comprises: a step of expressing and purifying the enzyme; and a step of mixing the enzyme and a precipitation solution and set up by a sitting drop method.
Abstract translation: 目的：提供一种新型的α - neoagarobios水解酶及其三维结构，以节省预处理成本并提高产量。 构成：α-新罗高氏菌素水解酶含有天冬氨酸52残基，天冬氨酸207残基和谷氨酸265残基。 酶还含有精氨酸235残基，丝氨酸358残基和酪氨酸359残基。 用于结晶α-新天鹅座水解酶的方法包括：表达和纯化酶的步骤; 以及将酶和沉淀溶液混合并通过坐式滴定法混合的步骤。
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95.发明授权사카로파거스 데그러단스 2-40의 조효소를 이용한 3,6-안하이드로-Ｌ-갈락토오스와 갈락토오스 생산 및 3,6-안하이드로-Ｌ-갈락토오스 정량방법 有权使用Saccharophagus degradationans 2-40的粗酶酶法制备3,6-脱水-L-半乳糖和半乳糖，并使用3,6-脱水-L-半乳糖的定量分析方法
公开(公告)号：KR101087265B1
公开(公告)日：2011-11-29
申请号：KR1020090130081
申请日：2009-12-23
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 해양 미생물인 사카로파거스데그러단스 2-40로부터 생산된 조효소액을 세포내 조효소액과 세포외 조효소액으로 분획화하는 단계, 상기 분획화된 조효소액으로 아가로스를 분해하여 당화하는 효소의 활성을 측정하는 단계 및 최종 분해 산물인 갈락토오스와 3,6-안하이드로-L-갈락토오스를 기체 크로마토그라피/질량분석기를 이용하여 동시에 독립적으로 정량화하는 분석방법을 제공한다.
본 발명의 해양 미생물로부터 생산된 조효소액을 이용한 아가로스를 분해하는 기법을 이용하면 아가로스 기질로부터 이를 구성하는 단당류인 갈락토오스와 3,6-안하이드로-L-갈락토오스를 분해 산물로 획득할 수 있다. 또한, 본 발명에 따르면 조효소에 의한 최종 분해 산물인 갈락토오스와 3,6-안하이드로-L-갈락토오스를 기체 크로마토그래피/질량분석법을 이용하여 독립적으로 정량화할 수 있다.-
Patent Agency Ranking96.发明公开
公开(公告)号：KR1020110085668A
公开(公告)日：2011-07-27
申请号：KR1020100005576
申请日：2010-01-21
Applicant: 삼성전자주식회사 , 고려대학교 산학협력단
IPC: C07K14/195 , C07K14/32 , C12P19/04 , C12N9/14
CPC classification number: C12P7/10 , C07K14/195 , C07K14/32 , C07K14/37 , C12N9/2437 , C12N9/248 , C12P19/02 , C12P19/14 , Y02E50/16 , Y02E50/343
Abstract: PURPOSE: An isolated protein and a composition containing the same for enhancing polysaccharide hydrolase activity are provided to enhance hydrolysis ability and to reduce the use of hydrolase. CONSTITUTION: An isolated protein enhances polysaccharide hydrolase activity and has polysaccharide binding ability. The protein contains GH45(glycoside hydrolase 45) or pollen-allergen domain. The polysaccharide hydrolase is cellulase, hemicellulase, alpha-amylase, glycoamylase, pectinase, xylanase, or cellobiase. The GH45 domain or pollen allergen domain is isolated from expansin, expansin-like protein, or bacterial protein.
Abstract translation: 目的：提供分离的蛋白质和含有该蛋白质的组合物，以增强多糖水解酶活性，以提高水解能力并减少水解酶的使用。 构成：分离蛋白提高多糖水解酶活性，具有多糖结合能力。 该蛋白质含有GH45（糖苷水解酶45）或花粉 - 变应原结构域。 多糖水解酶是纤维素酶，半纤维素酶，α-淀粉酶，糖酵解酶，果胶酶，木聚糖酶或纤维二糖酶。 从扩展蛋白，扩展蛋白样蛋白或细菌蛋白分离GH45结构域或花粉过敏原结构域。
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公开(公告)号：KR1020100137674A
公开(公告)日：2010-12-31
申请号：KR1020090055838
申请日：2009-06-23
Applicant: 고려대학교 산학협력단
IPC: A61K31/351 , A61P3/06 , A61P9/10 , A23L1/30
Abstract: PURPOSE: A composition for reducing cholesterol containing linalool is provided to reduce expression of HMG-CoA reductase which relates to cholesterol biosynthesis. CONSTITUTION: A composition for reducing cholesterol contains linalool as an active ingredeint. The composition reduces the expression of SREBP, SCAP, and S1P expression and also reduces cholesterol content by increasing Insig-1 gene expression. A pharmaceutical composition for preventing and treating hyperlipidemia contains the composition for reducing cholesterol as an active ingredient. A pharmaceutical composition for preventing and treating arteriosclerosis contains the composition for reducing cholesterol as an active ingredient.
Abstract translation: 目的：提供一种用于降低胆固醇的芳樟醇的组合物，以减少与胆固醇生物合成相关的HMG-CoA还原酶的表达。 构成：降低胆固醇的组合物含有芳樟醇作为活性成分。 该组合物降低SREBP，SCAP和S1P表达的表达，并通过增加Insig-1基因表达降低胆固醇含量。 用于预防和治疗高脂血症的药物组合物含有降低胆固醇作为活性成分的组合物。 用于预防和治疗动脉硬化的药物组合物含有降低胆固醇作为活性成分的组合物。
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公开(公告)号：KR1020100108241A
公开(公告)日：2010-10-06
申请号：KR1020100026454
申请日：2010-03-24
Applicant: 고려대학교 산학협력단
CPC classification number: C12N9/2402 , C12N9/24 , C12P19/14
Abstract: PURPOSE: A novel alpha-neo aragobios hydrolase is provided to reduce pretreatment cost for biomass and to produce galactose and AHG. CONSTITUTION: An alpha-neo agarobiose hydrolase has an amino acid sequence selected from sequence numbers 1-11. The hydrolase has an amino acid sequence of sequence number 4. The hydrolase is obtained by culturing Saccharophagus degradans and collecting from the culture medium of Saccharophagus degradans. A gene encoding the hydrolase is denoted by sequence number 12.
Abstract translation: 目的：提供一种新型的α-新阿拉伯糖蛋白水解酶，以降低生物质的预处理成本并产生半乳糖和AHG。 构成：α-neo琼脂二糖水解酶具有选自序列号1-11的氨基酸序列。 水解酶具有序列号4的氨基酸序列。水解酶是通过培养酵母属降解物并从酿酒酵母的培养基中收集获得的。 编码水解酶的基因由序列号12表示。
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公开(公告)号：KR1020100103189A
公开(公告)日：2010-09-27
申请号：KR1020090021670
申请日：2009-03-13
Applicant: 고려대학교 산학협력단
Abstract: PURPOSE: A medium composition for enhancing flavonoid biosynthesis of plants is provided to effectively enhance flavonoid synthesis without inhibition of plant growth. CONSTITUTION: A medium composition for enhancing flavonoid synthesis in plants contains 200-300mM of sucrose. The plant is lemon balm herb or Perilla frutescens. The medium composition for enhancing the flavonoid synthesis of plants contains 200-300mM of sucrose and phytohormone. A medium composition of lemon balm herb contains 200-300mM of sucrose and ethylene(ACC). A method for producing flavonoid comprises: a step of culturing plants in the medium for 3-20 days; and a step of extracting flavonoid from the cultured plant.
Abstract translation: 目的：提供用于增强植物类黄酮生物合成的培养基组合物，以有效增强黄酮合成而不抑制植物生长。 构成：用于增强植物中类黄酮合成的培养基组合物含有200-300mM蔗糖。 植物是柠檬香草或紫苏。 用于增强植物类黄酮合成的培养基组合物含有200-300mM的蔗糖和植物激素。 柠檬香草中草药含有200-300mM蔗糖和乙烯（ACC）。 一种生产类黄酮的方法包括：在培养基中培养植物3-20天的步骤; 并从培养的植物中提取黄酮类化合物的步骤。
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公开(公告)号：KR1020090018404A
公开(公告)日：2009-02-20
申请号：KR1020070082816
申请日：2007-08-17
Applicant: 고려대학교 산학협력단
Abstract: An increasing method of meat tenderization using supercritical carbon dioxide is provided to increase economic efficiency of the process through the energy reduction by obtaining a clear tenderization increasing effect using physical and chemical properties of a supercritical carbon dioxide fluid although important process variables pressure and temperature are maintained to relatively low levels. An increasing method of meat tenderization comprises the step of treating meat with supercritical carbon dioxide. The increasing method of meat tenderization comprises treating the meat with the supercritical carbon dioxide at a critical temperature of 31.1 deg.C and a pressure of 73 atm or more. The increasing method of meat tenderization comprises treating the meat with the supercritical carbon dioxide at a temperature of 31.1 to 35 deg.C and a pressure of 73 to 150 atm for 5 to 20 minutes. The meat is seasoned pork.
Abstract translation: 提供使用超临界二氧化碳的肉嫩化的增加方法，通过使用超临界二氧化碳流体的物理和化学性质获得明显的嫩化增加效果，通过能量减少来提高该方法的经济效率，尽管重要的过程变量压力和温度保持 到相对较低的水平。 肉嫩化的增加方法包括用超临界二氧化碳处理肉的步骤。 肉嫩化的增加方法包括在临界温度31.1℃和73atm以上的压力下用超临界二氧化碳处理肉。 肉嫩化的增加方法包括在31.1至35℃的温度和73至150atm的压力下用超临界二氧化碳处理肉类5至20分钟。 肉是经验丰富的猪肉。
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