Abstract:
PURPOSE: A method for screening a material which induces skin irritation by yellow dust is provided to reduce skin irritative factor expression and to reduce skin irritation by yellow dust. CONSTITUTION: A method for screening a material which induces skin irritation by yellow dust comprises: a step of treating yellow dust to keratinocytes; and a step of screening a gene of which expression level is significantly changed comparing with the expression of normal keratinocytes through RT-PCR. The material which induces skin irritation by yellow dust is AhR or CYP. A method for screening a material which relieves skin irritation by yellow dust comprises: a step of treating yellow dust to test cells to induce overexpression of an irritative factor; a step of treating a candidate material to the overexpressed cells; a step of measuring expression level of the irritative factor by RT-PCR. The material which relieves skin irritation by yellow dust is Trichosanthis semen. An external use skin composition for relieving skin irritation contains 0.001-1 weight% of Trichosanthis semen.
Abstract:
A method for inducing itch and a method for screening an itch inhibiting material using glucosylsphingosine are provided to be useful for studying and understanding the itch, thereby being very usefully used for developing a therapeutic agent for the itch and evaluating the effect of a medicine. A method for inducing itch comprises a step of administering at least one compound selected from the group consisting of glucosylsphingosine and a derivative thereof into an animal except human. A method for screening an itch inhibiting material comprises the steps of: (a) administering an itch inhibition candidate material to an animal where the itch is induced by administering at least one compound selected from the group consisting of the glucosylsphingosine and the derivative thereof; and (b) counting a number when the animal scratches. A method for evaluating the itch inhibition effect comprises the steps of: (a) administering an itch inhibition material to an animal where the itch is induced by administering at least one compound selected from the group consisting of the glucosylsphingosine and the derivative thereof; and (b) counting a number when the animal scratches. A composition for inducing itch comprises at least one compound selected from the group consisting of the glucosylsphingosine and the derivative thereof as an effective ingredient.