公开(公告)号：US10073076B2

公开(公告)日：2018-09-11

申请号：US15312054

申请日：2015-05-20

Applicant: HAMAMATSU PHOTONICS K.K.

Inventor： Naoya Matsumoto ,  Koyo Watanabe ,  Hirotoshi Terada

IPC: G01B21/00 ,  G01N33/483 ,  G01B11/24 ,  G02B21/00 ,  G01N21/41 ,  G02B5/32 ,  G02B27/00 ,  G03H1/00 ,  G03H1/08 ,  G03H1/22 ,  G01N21/64 ,  G01N21/63

CPC classification number: G01N33/4833 ,  G01B11/24 ,  G01N21/41 ,  G01N21/636 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2021/6471 ,  G01N2201/0675 ,  G02B5/32 ,  G02B21/00 ,  G02B21/16 ,  G02B27/0068 ,  G03H1/0005 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0072

Abstract: A photostimulation apparatus includes an objective lens arranged to face a biological object, a light source configured to output light to be radiated toward the biological object via the objective lens, a shape acquisition unit configured to acquire information about a shape with a refractive index difference in the biological object, a hologram generation unit configured to generate aberration correction hologram data for correcting aberrations due to the shape with the refractive index difference on the basis of the information acquired by the shape acquisition unit, and a spatial light modulator on which a hologram based on the aberration correction hologram data is presented and which modulates the light output from the light source.

公开(公告)号：US09874737B2

公开(公告)日：2018-01-23

申请号：US14890243

申请日：2014-05-07

Applicant: Forschungszentrum Mikroskopie (FZM), LuciaOptics gemeinnützige UG

Inventor： Gerrit Best ,  Christoph Cremer ,  Sabrina Rossberger ,  Stefan Dithmar

IPC: G02B21/36 ,  G01N21/64 ,  G02B21/00 ,  G02B21/16 ,  G02B27/58

CPC classification number: G02B21/367 ,  G01N21/6445 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/361 ,  G02B27/58

Abstract: A fluorescence microscope for obtaining super-resolution images of a sample labeled with at least one fluorescent label by combining localization microscopy and structured illumination microscopy is provided. The fluorescence microscope includes one or more light sources, an illumination system having a structured illumination path for illuminating the sample with structured illumination light and a localization illumination path for illuminating the sample with localization illumination light.

公开(公告)号：US09733461B2

公开(公告)日：2017-08-15

申请号：US14580133

申请日：2014-12-22

Applicant: OLYMPUS CORPORATION

Inventor： Yasunari Matsukawa

IPC: H04N9/47 ,  H04N7/18 ,  G02B21/00 ,  G02B21/16 ,  G01N21/64

CPC classification number: G02B21/0032 ,  G01N21/6458 ,  G01N2021/6471 ,  G01N2201/0675 ,  G02B21/0076 ,  G02B21/16

Abstract: To avoid unstable light radiation during switching of a phase modulation amount and stimulate desired stimulation points simultaneously, provided is a photo-stimulator which includes an AOM switching on/off of radiation of stimulation light to a specimen S; an LCOS-SLM being capable of modulating a phase of stimulation light once radiation to the specimen S has been turned on by the AOM; and a controller controlling the LCOS-SLM to switch a phase modulation amount of stimulation light and controlling the AOM to switch on/off of radiation of stimulation light, wherein the controller causes the AOM to turn off radiation of stimulation light before the LCOS-SLM starts switching a phase modulation amount, and causes the AOM to turn on radiation of stimulation light after the LCOS-SLM completes switching a phase modulation amount.

公开(公告)号：US09719927B2

公开(公告)日：2017-08-01

申请号：US15311311

申请日：2015-05-08

Applicant: TOHOKU UNIVERSITY ,  NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY

Inventor： Akihide Hibara ,  Manabu Tokeshi ,  Osamu Wakao

IPC: G01N21/64 ,  G02F1/13 ,  G01N33/542

CPC classification number: G01N21/6445 ,  G01J3/0224 ,  G01J4/00 ,  G01N21/64 ,  G01N21/6456 ,  G01N33/542 ,  G01N2201/0675 ,  G01N2201/0683 ,  G02F1/13

Abstract: An excitation light source emits excitation light to a target sample. An image sensor includes pixels arranged one-dimensionally or two-dimensionally, and receives measurement light from the sample according to the excitation light. A polarization selector arranged between the sample and image sensor includes pixels arranged one-dimensionally or two-dimensionally. Each pixel receives a corresponding portion of the measurement light, selects light having a polarization direction that corresponds to a driving signal applied to the pixels, and supplies this light to the image sensor. A measurement control unit supplies the cyclic driving signal having a first period T1, and acquires data I1, I2, I3, and I4 from each pixel of the image sensor for each exposure time segment T2=T1/4 obtained by dividing the first period T1 by 4.

公开(公告)号：US20160202178A1

公开(公告)日：2016-07-14

申请号：US14594956

申请日：2015-01-12

Applicant: Verily Life Sciences LLC

Inventor： Victor Marcel Acosta ,  Adam Backer ,  John D. Perreault ,  Daniele Paolo Piponi

IPC: G01N21/25 ,  G01J3/02 ,  G02B21/36

CPC classification number: G02B21/0064 ,  G01J3/0205 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/0237 ,  G01J3/18 ,  G01J3/502 ,  G01J2003/1278 ,  G01J2003/468 ,  G01N21/255 ,  G01N21/27 ,  G01N2201/06113 ,  G01N2201/0675 ,  G02B5/1828 ,  G02B6/2931 ,  G02B21/06 ,  G02B21/361 ,  G02B26/0808 ,  G02F1/133553 ,  G02F1/23 ,  G02F1/292 ,  G06T7/11 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30004 ,  H04N5/2256 ,  H04N5/332

Abstract: An imaging system includes a light source configured to illuminate a target and a camera configured to image light responsively emitted from the target and reflected from a spatial light modulator (SLM). The imaging system is configured to generate high-resolution, hyperspectral images of the target. The SLM includes a refractive layer that is chromatically dispersive and that has a refractive index that is controllable. The refractive index of the refractive layer can be controlled to vary according to a gradient such that light reflected from the SLM is chromatically dispersed and spectrographic information about the target can be captured using the camera. Such a system could be operated confocally, e.g., by incorporating a micromirror device configured to control a spatial pattern of illumination of the target and to modulate the transmission of light from the target to the camera via the SLM according to a corresponding spatial pattern.

公开(公告)号：US20160011113A1

公开(公告)日：2016-01-14

申请号：US14795491

申请日：2015-07-09

Applicant: Carl Zeiss Microscopy GmbH

Inventor： Martin Müller ,  Nico Presser ,  Gunter Möhler

IPC: G01N21/64

CPC classification number: G01N21/6408 ,  G01N21/6458 ,  G01N2201/06113 ,  G01N2201/067 ,  G01N2201/0675 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/008

Abstract: Method for the operation of a laser scanning microscope. The microscope includes an illumination beam path in which at least one illumination light source is arranged, a detection beam path in which at least one photomultiplier (PMT) is incorporated as detector, and a control unit for controlling fluorescence experiments. A sample is alternately illuminated at high intensity via the control unit, and the fluorescence decay behavior of sample points and/or sample regions is subsequently detected. The PMT is switched on and off depending on the illumination mode by the control unit via a switch directly in the high-voltage supply of the PMT.

Abstract translation: 激光扫描显微镜操作方法。 显微镜包括其中布置有至少一个照明光源的照明光束路径，其中结合有至少一个光电倍增管（PMT）作为检测器的检测光束路径，以及用于控制荧光实验的控制单元。 通过控制单元以高强度交替照射样品，随后检测样品点和/或样品区域的荧光衰减行为。 PMT根据控制单元的照明模式通过直接在PMT的高压电源中的开关来接通和关断。

公开(公告)号：US20140107944A1

公开(公告)日：2014-04-17

申请号：US14054149

申请日：2013-10-15

Applicant: Purdue Research Foundation

Inventor： Dor Ben-Amotz ,  Bradley J. Lucier ,  Gregery T. Buzzard ,  David Wilcox ,  Ping Wang

IPC: G01N21/25

CPC classification number: G01N21/25 ,  G01J3/44 ,  G01N21/65 ,  G01N2201/0675 ,  G01N2201/129

Abstract: A method for measuring a sample to identify a chemical includes receiving respective spectra for each of a plurality of chemicals. Using a processor, a plurality of binary mathematical filters are computed using the received spectra. A spatial light modulator is adjusted according to a selected mathematical filter. Light that has interacted with the sample is dispersed over the surface of the spatial light modulator, so that the spatial light modulator provides light at wavelengths corresponding to the selected mathematical filter. The light provided by the spatial light modulator is measured to provide a score corresponding to the selected mathematical filter. Filter scores are combined to determine a chemical amount. The processor can operate detection apparatus having a light source, an objective for focusing source light onto the sample, a spatial light modulator, and a detector for detecting the modulator output.

Abstract translation: 用于测量样品以鉴定化学品的方法包括接收多种化学品中的每一种的相应光谱。 使用处理器，使用接收的光谱来计算多个二进制数学滤波器。 根据所选择的数学滤波器调整空间光调制器。 已经与样品相互作用的光分散在空间光调制器的表面上，使得空间光调制器提供对应于所选择的数学滤波器的波长的光。 测量由空间光调制器提供的光以提供对应于所选数学滤波器的分数。 过滤分数结合以确定化学量。 处理器可以操作具有光源的检测装置，用于将源光聚焦到样本上的物镜，空间光调制器和用于检测调制器输出的检测器。

公开(公告)号：US20130003068A1

公开(公告)日：2013-01-03

申请号：US13174815

申请日：2011-07-01

Applicant: Shankar Krishnan ,  Haiming Wang

Inventor： Shankar Krishnan ,  Haiming Wang

IPC: G01N21/55 ,  G01J1/42

CPC classification number: G01N21/55 ,  G01B11/065 ,  G01B2210/56 ,  G01J3/021 ,  G01J3/0229 ,  G01J3/42 ,  G01N21/00 ,  G01N21/211 ,  G01N2021/213 ,  G01N2021/214 ,  G01N2201/0675

Abstract: A spectroscopic instrument for conducting multi-wavelength, multi-azimuth, multi-angle-of-incidence readings on a substrate, the instrument having a broadband light source for producing an illumination beam, an objective for directing the illumination onto the substrate at multiple azimuth angles and multiple angels-of-incidence simultaneously, thereby producing a reflection beam, an aperture plate having an illumination aperture and a plurality of collection apertures formed therein for selectively passing portions of the reflection beam having desired discreet combinations of azimuth angle and angle-of-incident, a detector for receiving the discreet combinations of azimuth angle and angle-of-incident and producing readings, and a processor for interpreting the readings.

Abstract translation: 一种用于在衬底上进行多波长，多方位角，多角度入射读数的光谱仪器，该仪器具有用于产生照明光束的宽带光源，用于将多个方位角的照明引导到衬底上的物镜 角度和多个入射角，从而产生反射光束，具有照明孔的孔板和形成在其中的多个收集孔，用于选择性地通过具有期望的方位角和角度的离散组合的反射光束的部分 - 用于接收方位角和入射角的离散组合并产生读数的检测器，以及用于解释读数的处理器。

公开(公告)号：US20120307247A1

公开(公告)日：2012-12-06

申请号：US13149620

申请日：2011-05-31

Applicant: Piau Siong Tan ,  Xiaocong Yuan

Inventor： Piau Siong Tan ,  Xiaocong Yuan

IPC: G01N21/55

CPC classification number: G01N21/648 ,  G01N21/45 ,  G01N21/6458 ,  G01N2201/0675 ,  G02B5/008 ,  G02B21/16

Abstract: A fluorescence microscopy method and system, the method comprising the steps of applying optical vortices to a metal surface for generating surface plasmon resonance (SPR) waves at the metal surface; and collecting fluorescence light excited by the SPR waves; wherein a dynamic characteristic of the optical vortices is controlled for controlling interference patterns of the SPR waves.

Abstract translation: 一种荧光显微镜方法和系统，所述方法包括以下步骤：将光学旋涡应用于金属表面，以在金属表面产生表面等离子体共振（SPR）波; 并收集由SPR波激发的荧光; 其中控制光学旋涡的动态特性以控制SPR波的干涉图案。

公开(公告)号：US20120236310A1

公开(公告)日：2012-09-20

申请号：US13497290

申请日：2010-09-22

Applicant: Frédéric Lesage ,  Samuel Bélanger ,  Maxime Abran ,  Jean Provost

Inventor： Frédéric Lesage ,  Samuel Bélanger ,  Maxime Abran ,  Jean Provost

IPC: G01N21/59

CPC classification number: G01N21/4795 ,  A61B5/0073 ,  A61B5/0091 ,  A61B5/4312 ,  G01N21/6456 ,  G01N21/763 ,  G01N2021/1787 ,  G01N2021/479 ,  G01N2201/0675

Abstract: A system and method for optical data acquisition of an illuminated turbid medium object, the system comprises a variable structured light detector and a controller. The light detector is adapted to retrieve light from a plurality of detection points of an output surface of the illuminated turbid medium object with a plurality of detection patterns. The controller is adapted to control the variable structured light detector for the variable structured light detector to use a detection pattern corresponding to an illumination pattern of the illuminated turbid medium object. The light detector is further adapted to optically measure a combination of retrieved light from the plurality of detection points as an optical measurement. The controller is further adapted to store an illumination pattern identifier indicative of the illumination pattern, a detection pattern identifier indicative of the detection pattern and the corresponding optical measurement.

Abstract translation: 一种用于光学数据采集的照明混浊介质物体的系统和方法，该系统包括可变结构光检测器和控制器。 光检测器适于用多个检测图案从照明的浑浊介质物体的输出表面的多个检测点检索光。 控制器适于控制可变结构光检测器的可变结构光检测器，以使用与照明混浊介质物体的照明图案相对应的检测图案。 光检测器还适于光学测量来自多个检测点的检索光的组合作为光学测量。 控制器还适于存储指示照明模式的照明模式标识符，指示检测模式的检测模式标识符和对应的光学测量。