公开(公告)号：WO2017072251A1

公开(公告)日：2017-05-04

申请号：PCT/EP2016/075973

申请日：2016-10-27

Applicant: LIFE TECHNOLOGIES AS

Inventor： ALMAASBAK, Hilde, Kjersti ,  AARVAK, Tanja ,  BERNSTROEM, Kerstin ,  AAMELLEM, Oystein

IPC: C12N5/0783

CPC classification number: C12N5/0636 ,  C12N2501/51 ,  C12N2501/515 ,  C12N2501/599

Abstract: This invention relates, inter alia , to compositions of expanded T cell populations, methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations present in mixed T cell populations, as well as T cell subpopulations produced by methods for the invention.

Abstract translation: 本发明尤其涉及扩大T细胞群体的组合物，扩增T细胞群体的方法和使用此类细胞群体的方法。 在一些方面，本发明涉及用于选择性扩增存在于混合T细胞群体中的T细胞亚群以及通过本发明的方法产生的T细胞亚群的组合物和方法。

公开(公告)号：WO2023079524A2

公开(公告)日：2023-05-11

申请号：PCT/IB2022/060693

申请日：2022-11-07

Applicant: LIFE TECHNOLOGIES AS

Inventor： OOI, Huey Wen ,  LUNDBERG, Pontus ,  BOESCHEN, Maxi-Lu ,  ALMAASBAK, Hilde ,  HERENG, Tuva Holt ,  STAV-NORAAS, Tor-Espen ,  LANDSVERK, Ole ,  FONNUM, Geir

IPC: C07K16/28 ,  A61K47/60 ,  A61K47/68 ,  A61K47/69

Abstract: The invention relates to polymer-interaction molecule (e.g., antibodies) conjugates and uses of such conjugates for delivery of interaction molecules to cells. The invention also relates uses polymer-interaction molecule (e.g., antibodies) conjugates for eliciting cellular responses (e.g., induction of cell proliferation).

公开(公告)号：WO2017211913A1

公开(公告)日：2017-12-14

申请号：PCT/EP2017/063879

申请日：2017-06-07

Applicant: LIFE TECHNOLOGIES AS ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： ANDERSSON, Kristian ,  FONNUM, Geir ,  HEIL, Korbinian

IPC: C08F212/08 ,  C08F220/58

Abstract: This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide- oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.

Abstract translation: 本发明涉及用于固相寡核苷酸合成的聚合物颗粒。 寡核苷酸可以通过具有酰胺 - 寡聚乙二醇 - 胺结构的接头连接至颗粒。 在寡核苷酸合成过程中，可以认为颗粒充当固体支持物。 还公开了用于制备这种聚合物颗粒的方法，包含这种颗粒的组合物和系统及其用途。

公开(公告)号：WO2017116910A1

公开(公告)日：2017-07-06

申请号：PCT/US2016/068067

申请日：2016-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： NORDERHAUG, Lars ,  VARELA-ROHENA, Angel ,  JONES, Nephi D.

IPC: B03C1/01 ,  B03C1/28 ,  B03C1/30 ,  B01L3/00 ,  C12M1/00 ,  A61M1/36

CPC classification number: B03C1/01 ,  A61M1/3618 ,  B01L3/505 ,  B01L2200/0668 ,  B01L2400/043 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26

Abstract: Methods of separating magnetic particles from a fluid include introducing a fluid mixture that includes a liquid media, a biological component, and magnetic particles into an internal compartment of a container through an inlet, allowing at least a portion of the mixture to travel around a partition formed within the internal compartment and then exit the internal compartment through an outlet on the opposite side of the partition, and applying a magnetic field to the mixture in the internal compartment so that the magnetic particles are retained within the container or internal compartment thereof by the magnetic field. The partition is formed by permanently or reversibly securing upper and lower container walls together, such as by welding or pressing, between the inlet and the outlet such that the media and biological component must flow around the partition and through the magnetic field to exit the internal compartment through the outlet.

Abstract translation: 从流体分离磁性颗粒的方法包括通过入口将包括液体介质，生物成分和磁性颗粒的流体混合物引入容器的内部隔室中，从而允许至少一部分 的混合物在内部隔室内形成的隔板周围行进，然后通过隔板另一侧的出口离开内部隔室，并向内部隔室的混合物施加磁场，以使磁性颗粒保留在内部 容器或其内部隔室受到磁场的影响。 通过将入口和出口之间的上部容器壁和下部容器壁永久地或可逆地固定在一起（例如通过焊接或挤压）形成隔板，使得介质和生物成分必须在隔板周围流动并穿过磁场流出内部 通过出口的隔间。

公开(公告)号：WO2017013138A8

公开(公告)日：2017-01-26

申请号：PCT/EP2016/067218

申请日：2016-07-19

Applicant: LIFE TECHNOLOGIES AS

Inventor： FONNUM, Geir ,  LUNDBERG, Per, Nils, Pontus, ,  GÖKMEN, Muhammed, Talha ,  LARSEN, Synne ,  GLOMM, Wilhelm ,  JOHNSEN, Heidi ,  SCHMID, Ruth ,  KILAAS, Lars

IPC: C08F120/54 ,  C08F2/38 ,  C08F265/10

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non- crosslinked oligomers of poly Ν,Ν-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

Abstract translation: 本发明涉及单分散交联水凝胶聚合物颗粒，其包含由（a）亲水性乙烯基单体形成的聚合物; 和（b）包含至少两个乙烯基的交联剂。 本发明还涉及包含多个N，N-二甲基丙烯酰胺的非交联低聚物的Z-平均直径为100nm至1500nm的单分散种子颗粒。 还提供了形成单分散交联水凝胶聚合物颗粒和单分散种子颗粒的方法。

公开(公告)号：WO2017013138A1

公开(公告)日：2017-01-26

申请号：PCT/EP2016/067218

申请日：2016-07-19

Applicant: LIFE TECHNOLOGIES AS

Inventor： FONNUM, Geir ,  LUNDBERG, Per, Nils, Pontus, ,  GÖKMEN, Muhammed, Talha ,  LARSEN, Synne ,  GLOMM, Wilhelm ,  JOHNSEN, Heidi ,  SCHMID, Ruth ,  KILAAS, Lars

IPC: C08F120/54 ,  C08F2/38 ,  C08F265/10

CPC classification number: C08F120/54 ,  C08F265/10 ,  C08F220/56

Abstract: This invention relates to monodisperse cross-linked hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse seed particles with a Z-average diameter of from 100 nm to 1500 nm that comprise a plurality of non- crosslinked oligomers of poly Ν,Ν-dimethylacrylamide. Also provided are methods of forming the monodisperse cross-linked hydrogel polymer particles and monodisperse seed particles.

Abstract translation: 本发明涉及包含由（a）亲水性乙烯基单体形成的聚合物的单分散交联水凝胶聚合物颗粒; 和（b）包含至少两个乙烯基的交联剂。 本发明还涉及Z平均直径为100nm至1500nm的单分散种子颗粒，其包含多个未交联的聚N，N-二甲基丙烯酰胺的低聚物。 还提供了形成单分散交联水凝胶聚合物颗粒和单分散种子颗粒的方法。

公开(公告)号：WO2017004559A1

公开(公告)日：2017-01-05

申请号：PCT/US2016/040767

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： FONNUM, Geir ,  MENCHEN, Steven M. ,  LUI, Alfred ,  XIANG, Guobing ,  LARSEN, Synne

IPC: C12Q1/68 ,  G01N33/543

CPC classification number: C12Q1/6876 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/197 ,  C12Q2527/125 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network. Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.

Abstract translation: 聚合物基质，例如聚合物涂层或聚合物水凝胶网络，包括羧基部分，其可以用作受体或分析物分子可以连接到的缀合位点。 在一个实例中，聚合物底物包括具有链烷酸部分或其衍生物的聚丙烯酰胺聚合物网络，其可与羧基活化化合物反应，以在聚丙烯酰胺网络上提供活化的链烷酸酯部分。 胺封端的核酸可以与活化的链烷酸酯部分反应，以通过烷基酰胺部分将核酸捕获到聚合物网络。

公开(公告)号：WO2017004556A1

公开(公告)日：2017-01-05

申请号：PCT/US2016/040755

申请日：2016-07-01

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： FONNUM, Geir ,  MENCHEN, Steven, M. ,  GOKMEN, Talha, M. ,  LUNDBERG, Pontus ,  THWAR, Prasanna Krishnan ,  LUI, Alfred ,  LU, Lily ,  HINZ, Wolfgang ,  HUSABOE, Lene ,  BREIVOLD, Elisabeth ,  MOLTEBERG, Astrid, Evenroed ,  LARSEN, Synne

IPC: C08F220/58 ,  C08F8/12

CPC classification number: C08J3/075 ,  C08F8/12 ,  C08F220/58 ,  C08F220/70 ,  C08F2230/085 ,  C08J2333/26 ,  C08F222/38

Abstract: A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.

Abstract translation: 聚合物基材如聚合物颗粒由羧基官能单体形成。 在一个实例中，羧基官能单体具有保护基代替羧基的OH。 一旦单体聚合，就可以除去这种保护基团，从而提供具有羧基官能位点的聚合物网络。 这种位点可用于将其它功能性附加到聚合物基材上。

公开(公告)号：WO2013176730A1

公开(公告)日：2013-11-28

申请号：PCT/US2013/029450

申请日：2013-03-06

Applicant: Life Technologies AS

Inventor： SCHJETNE, Karoline ,  AARVAK, Tanja ,  NORDERHAUG, Lars

IPC: C07K16/28

CPC classification number: C07K16/2803 ,  C07K16/00 ,  C07K16/2809 ,  C07K16/2896 ,  C07K2317/53 ,  C07K2317/55 ,  C12N5/0636

Abstract: Provided are methods and composition useful in isolating or otherwise preparing a population of target cells. In part, it relates to recombinant antibodies comprising an exogenous proteolytic cleavage site and nucleic acid molecules encoding the antibodies.

Abstract translation: 提供了可用于分离或以其它方式制备靶细胞群体的方法和组合物。 其部分涉及包含外源蛋白水解切割位点和编码抗体的核酸分子的重组抗体。

公开(公告)号：GB201609983D0

公开(公告)日：2016-07-20

申请号：GB201609983

申请日：2016-06-08

Applicant: THERMO FISHER SCIENT GENEART GMBH AND LIFE TECHNOLOGIES AS

Abstract: This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide-oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.