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公开(公告)号：KR1020040039800A
公开(公告)日：2004-05-12
申请号：KR1020020068008
申请日：2002-11-05
Applicant: 한국전자통신연구원
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q2537/143 , C12Q2527/113 , C12Q2527/101
Abstract: PURPOSE: A method for performing multiplex PCR with cyclic changes of PCR parameters is provided, thereby preventing production of primer dimer and non-specific product, and reducing the time for screening optimal multiplex PCR conditions. CONSTITUTION: A method for performing multiplex PCR from a sample comprises cyclic changes of PCR parameters including the maturation temperature and the elongation time of a primer, wherein the sample includes blood, blood plasma, circular DNA(vector), cDNA library, genome or cell containing the genome; the maturation temperature and elongation time of the primer are increased at regular intervals; the maturation temperature is increased according to the value of Tmax - Tmin per 1 cycle and the elongation time is increased according to the value of £(Lmax - Lmin) DNA polymerization rate of taq DNA polymerase(bp/sec)| (total cycle - 7), in which Lmax is the sequence size of a largest PCR product(bp) and Lmin is the sequence size of a smallest PCR product(bp); and the volume of the sample is less than 1 microliter.
Abstract translation: 目的：提供PCR参数循环变化进行多重PCR的方法，从而防止引物二聚体和非特异性产物的产生，并减少筛选最佳多重PCR条件的时间。 构成：从样品中进行多重PCR的方法包括PCR参数的循环变化，包括引物的成熟温度和延长时间，其中样品包括血液，血浆，环状DNA（载体），cDNA文库，基因组或细胞 含有基因组; 引物的成熟温度和延长时间以规则的间隔增加; 根据每1个循环的Tmax-Tmin的值增加成熟温度，并且根据taq DNA聚合酶（bp / sec）的£（Lmax-Lmin）DNA聚合速率的值增加延长时间| （总周期-7），其中Lmax是最大PCR产物（bp）的序列大小，Lmin是最小PCR产物（bp）的序列大小; 样品的体积小于1微升。
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公开(公告)号：KR1020030080468A
公开(公告)日：2003-10-17
申请号：KR1020020019109
申请日：2002-04-09
Applicant: 한국전자통신연구원
IPC: H01L21/00
Abstract: PURPOSE: A micro actuating apparatus for controlling fluid and a micro fluid control apparatus using the same are provided to be capable of optimizing the transfer of a biomass sample having a high viscosity and conductivity, improving response speed, and operating at low temperature. CONSTITUTION: A micro actuating apparatus for controlling fluid is provided with a silicon substrate(100a), an outer wall(101) formed at the upper portion of the silicon substrate by sequentially depositing a silicon oxide layer and a silicon layer, a mobile structure(102) including at least one pair of leaf springs having a restoring force, connected with the outer wall through both ends of the mobile structure, and an actuator formed at the upper portion of the silicon substrate for driving backward or forward the mobile structure by using the expansion of a heating element(106) due to the supply of voltage.
Abstract translation: 目的：提供一种用于控制流体的微型致动装置和使用其的微流体控制装置，其能够优化具有高粘度和电导率，改善响应速度和在低温下操作的生物质样品的转移。 构成：用于控制流体的微型致动装置设置有硅衬底（100a），通过依次沉积硅氧化物层和硅层而形成在硅衬底的上部的外壁（101），移动结构（ 102），其包括具有恢复力的至少一对板簧，通过移动结构的两端与外壁连接，以及形成在硅衬底的上部的致动器，用于通过使用来驱动移动结构 由于电压的供给导致的加热元件（106）的膨胀。
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公开(公告)号：KR1020150139685A
公开(公告)日：2015-12-14
申请号：KR1020140067592
申请日：2014-06-03
Applicant: 한국전자통신연구원
CPC classification number: G01N21/59 , G01N21/314 , G01N21/3151 , G01N33/49 , G01N2021/317 , G01N2201/061 , G01N2201/068
Abstract: 본발명인유체의성질을측정하는장치는, 유체가출입하는유체수용부의외부에서상기유체수용부중 측정영역으로제1 파장을갖는제1 광및 상기제1 파장보다길이가긴 제2 파장을갖는제2 광을조사하는발광부, 상기유체수용부의외부에서상기측정영역을통과한상기제1 광및 상기제2 광을수신하는수광부및 상기발광부가조사한상기제1 광및 상기제2 광의세기와상기수광부가수신한상기제1 광및 상기제2 광의세기를바탕으로유체의성질을측정하는측정부를포함한다.
Abstract translation: 本发明提供了一种用于测量流体性质的装置，该装置包括：发光单元，被配置为从容纳流体的流体的外侧发射具有第一波长的第一光和具有比第一波长长的第二波长的第二光 单元，其中流体流入和流出到流体容纳单元内的测量区域; 光接收单元，设置在所述流体容纳单元的外部，并被配置为接收通过所述测量区域的所述第一光和第二光; 以及测量单元，被配置为基于发光单元发射的第一光和第二光的强度以及光接收单元接收的第一光和第二光的强度来测量流体的性质。
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公开(公告)号：KR1020130103282A
公开(公告)日：2013-09-23
申请号：KR1020120116207
申请日：2012-10-18
Applicant: 한국전자통신연구원
CPC classification number: B03C1/32 , B03C1/0332 , G01N33/50
Abstract: PURPOSE: A demultiplexer and a method for separating cancer cell from blood are provided to separate cancer cells according to the kind of cancer by controlling magnetic force of ferromagnetism pattern or its flux after putting blood sample in a micro channel. CONSTITUTION: A demultiplexer (100) comprises: a channel (2) in which mixed liquid flows; and a ferromagnetism pattern (3) arranged under the bottom surface of the channel. The flux of the mixed liquid, or the magnetic force of the ferromagnetism pattern are changed according to the direction of the channel. A method for separating cancel cell from blood comprises the steps of: manufacturing mixed liquid comprising cancer cells combined with magnetic nano particle by mixing object blood with the magnetic nano particle which is combined with antibody specifically reacting with the cancer cell; putting the mixed liquid in the channel of which the ferromagnetism patterns are arranged on the bottom surface; and catching the cancer cells according to the kind by controlling the flux of the mixed liquid, or changing the magnetic force of the ferromagnetism pattern in the channel.
Abstract translation: 目的：提供多路分解器和从血液中分离癌细胞的方法，通过在将血液样本置入微通道后控制铁磁图案或其通量的磁力，根据癌症的种类分离癌细胞。 构成：解复用器（100）包括：混合液体流过的通道（2）; 以及布置在通道的底表面下方的铁磁图案（3）。 混合液体的通量或铁磁性图案的磁力根据通道的方向而改变。 一种从血液中分离灭活细胞的方法包括以下步骤：通过将目标血液与与癌细胞特异性反应的抗体组合的磁性纳米颗粒混合制备包含与磁性纳米颗粒结合的癌细胞的混合液体; 将混合液体放置在底面上布置有铁磁图案的通道中; 并通过控制混合液体的通量或改变通道中铁磁性图案的磁力，根据种类捕获癌细胞。
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Patent Agency Ranking
公开(公告)号：KR101223039B1
公开(公告)日：2013-01-17
申请号：KR1020090025206
申请日：2009-03-25
Applicant: 한국전자통신연구원
Abstract: 본 발명은 미세 필터의 제조 방법을 제공한다. 이 방법은 제 1 폭의 돌출부를 가지는 몰드를 형성하는 단계; 기판의 일면 상에 필터막을 형성하는 단계; 상기 필터막 상에 레지스트막을 코팅하는 단계; 상기 돌출부가 상기 레지스트막을 통해 상기 필터막과 닿도록 상기 몰드로 찍어 상기 레지스트막에 제 1 개구부를 형성하는 단계; 상기 레지스트막을 식각마스크로 이용하여 상기 필터막을 식각하여 상기 기판을 노출시키는 제 2 개구부를 형성하는 단계; 상기 레지스트막을 제거하는 단계; 및 상기 일면과 반대되는 면 쪽의 상기 기판의 일부를 제거하여 상기 필터막의 상기 개구부를 노출시키는 단계를 포함한다. 이 방법으로 리소그래피 공정으로 구현가능한 선폭보다 좁은 폭의 개구부를 가지는 미세한 필터를 재현성있게 경제적으로 제조할 수 있다.
바이오 분자 필터, 나노 구조, 나노임프린팅, 실리콘, 미세분석시스템-
公开(公告)号：KR101213223B1
公开(公告)日：2013-01-09
申请号：KR1020090061620
申请日：2009-07-07
Applicant: 한국전자통신연구원
CPC classification number: A61M37/0015 , A61B5/14514 , A61B5/685 , A61M2037/0053 , Y10T29/49885 , Y10T29/4998 , Y10T156/1062
Abstract: 본 발명에 따른 미세 바늘의 제조 방법은 일정한 단면을 가지며, 길이 방향으로 긴 중공심을 코팅액으로 코팅하고, 고형화하여 코팅층을 형성하는 단계, 상기 코팅층 표면에 시드 금속을 증착하는 단계, 상기 시드 금속 표면에 금속 도금을 수행하여 도금층을 형성하는 단계, 상기 도금층이 형성된 상기 중공심을 상기 길이 방향과 경사지게 잘라 표면경사를 형성하는 단계, 그리고 상기 중공심 및 코팅층을 제거하여 할로우를 형성하는 단계를 포함한다. 따라서, 최소 통증 제어가 가능하도록 직경, 길이, 경도, 경사각을 모두 갖추는 할로우 미세 바늘을 제작할 수 있으며, 중공심을 사용함으로써 바늘의 수직성과 바늘 내경의 균일성이 보장된다.
할로우, 마이크로 니들, 미세 바늘, 미세 실, 물유리-
公开(公告)号：KR1020120111878A
公开(公告)日：2012-10-11
申请号：KR1020110092825
申请日：2011-09-15
Applicant: 한국전자통신연구원
Abstract: PURPOSE: A microfluid control device for measuring glycosylated hemoglobin and a method for operating the same are provided to enable effectively quantitation of the glycosylated hemoglobin using trace of blood. CONSTITUTION: A microfluid control device for meausring glycosylated hemoglobin comprises: a first substrate(100); a first injection part(101) for injecting a blood sample; a second injection part(102) for injecting glucose enzyme; a glucose acid filter unit(103) which is connected to the first and second injection parts and collects glucose acid generated by reaction of the blood sample and glucose enzyme; a sensing chamber(104) which is connected to the glucose acid filter unit and electrochemically measures the concentration of hemoglobin and glycosylated hemoglobin in the blood sample; a discharge unit(105) for discharging the blood sample; a valve part(107) which controls the fluid flow between the sensing chamber part and discharging unit; and a second substrate(200) having a pump part(108).
Abstract translation: 目的：提供用于测量糖基化血红蛋白的微流控制装置及其操作方法，以便能够利用血液痕迹有效地定量糖基化血红蛋白。 构成：用于测定糖基化血红蛋白的微流体控制装置，包括：第一底物（100）; 用于注射血液样本的第一注射部分（101） 用于注射葡萄糖酶的第二注射部分（102）; 葡萄糖酸过滤器单元，连接到第一和第二注射部分，并收集由血液样品和葡萄糖酶反应产生的葡萄糖酸; 感测室（104），其连接到葡萄糖酸过滤器单元并电化学测量血液样品中血红蛋白和糖化血红蛋白的浓度; 用于排出血液样本的排出单元（105） 控制所述检测室部和所述排出部之间的流体流动的阀部（107） 和具有泵部（108）的第二基板（200）。
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公开(公告)号：KR1020110027013A
公开(公告)日：2011-03-16
申请号：KR1020090084911
申请日：2009-09-09
Applicant: 한국전자통신연구원
IPC: G01N33/493 , G01N21/27 , G01N21/41
CPC classification number: G01N21/78 , G01N21/8483 , G01N33/493 , G01N2201/0221 , G01N2201/0627 , G01N2201/0631
Abstract: PURPOSE: A portable reader which is able to read a chip is provided to prevent error in signal measurement by optical distribution fault. CONSTITUTION: A portable reader for urinal analysis comprises: a light emitting unit which contains a light emitting device and is able to irradiate; an integrated light splitter(250) for uniformly distributing light from the light emitting unit to each sensing area; a light receiving unit which receives light and converts the light into electrical signal; and a measuring unit for measuring the concentration according to the electric signal.
Abstract translation: 目的：提供能够读取芯片的便携式读取器，以防止光分配故障引起的信号测量误差。 构成：用于小便器分析的便携式读取器包括：发光单元，其包含发光装置并能够照射; 用于将光从发光单元均匀地分配到每个感测区域的集成光分路器（250）; 光接收单元，其接收光并将光转换成电信号; 以及用于根据电信号测量浓度的测量单元。
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公开(公告)号：KR1020100065031A
公开(公告)日：2010-06-15
申请号：KR1020090027378
申请日：2009-03-31
Applicant: 한국전자통신연구원
Abstract: PURPOSE: A bio chip and system for detecting a bio material are provided to safely detect bio material using dangerous medicine such as picric acid. CONSTITUTION: A bio chip(100) comprises: a bio sample injection unit(120) for injecting bio sample; a sample reaction unit(110) in which bio sample and reaction sample are mixed and reacted; a sample exhausting part(140) for discharging the bio sample and reaction sample; a first fluid channel(151) which is connected to the bio sample infection unit; a second fluid channel(152) connected to the reaction sample infection part; and a combining channel(153) which connects the first and second fluid channels. The reaction sample has explosiveness and is picric acid.
Abstract translation: 目的：提供用于检测生物材料的生物芯片和系统，以使用危险药物如苦味酸安全检测生物材料。 构成：生物芯片（100）包括：用于注射生物样品的生物样品注入单元（120） 生物样品和反应样品混合并反应的样品反应单元（110）; 用于排出生物样品和反应样品的样品排出部分（140）; 连接到所述生物样本感染单元的第一流体通道（151）; 连接到反应样本感染部分的第二流体通道（152） 以及连接第一和第二流体通道的组合通道（153）。 反应样品具有爆炸性，是苦味酸。
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