公开(公告)号：CA3134973A1

公开(公告)日：2020-10-01

申请号：CA3134973

申请日：2020-03-27

Applicant: FLUIDIGM CANADA INC

Inventor： LI STEPHEN K H ,  MAJONIS DANIEL ,  BARANOV VLADIMIR ,  ORNATSKY OLGA ,  ALLO BEDILU ,  BANDURA DMITRY ,  THOM COLIN ,  STELZER GREG ,  LOBODA ALEXANDER

IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

公开(公告)号：CA2852043C

公开(公告)日：2020-08-25

申请号：CA2852043

申请日：2012-10-26

Applicant: FLUIDIGM CANADA INC

Inventor： JONG RAYMOND

IPC: G01N15/10 ,  C12M1/34 ,  H01J49/04

Abstract: In a mass cytometer or mass spectrometer, a sample of elemental tagged particles is transferred from a dispersion to a gas flow through a carrier aerosol spray for atomization and ionization by inductively coupled plasma (ICP) source. The configuration of the sample transfer apparatus allow for total consumption of the sample by passing the sample spray through a deceleration stage to decelerate the spray of particles from its high velocity expansion. Following the deceleration stage, the decelerated sample of particles can be accelerated and focused through an acceleration stage for transferring into the ICP. This effectively improves the particle transfer between the sample spray and the ICP.

公开(公告)号：CA3112016A1

公开(公告)日：2020-03-19

申请号：CA3112016

申请日：2019-09-09

Applicant: FLUIDIGM CANADA INC ,  GOVERNING COUNCIL UNIV TORONTO

Inventor： CLOSSON TAUNIA ,  BARANOV VLADIMIR ,  WINNIK MITCHELL A

IPC: G01N15/10 ,  G01N33/50

Abstract: This disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.

公开(公告)号：CA3111824A1

公开(公告)日：2020-03-19

申请号：CA3111824

申请日：2019-09-10

Applicant: FLUIDIGM CANADA INC

Inventor： SANDKUIJL DAAF ,  LOBODA ALEXANDER ,  CAREW ADAM ,  LAKSHMAN MINALINI

IPC: H01J49/04 ,  G01N1/28 ,  H01J49/02 ,  H01J49/06 ,  H01J49/26

Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.

公开(公告)号：CA3104126A1

公开(公告)日：2019-12-26

申请号：CA3104126

申请日：2019-06-18

Applicant: FLUIDIGM CANADA INC ,  CORKUM PAUL

Inventor： CORKUM PAUL ,  LOBODA ALEXANDER

IPC: H01J49/00 ,  G01N33/574 ,  G01N33/60 ,  G01N33/68 ,  G02B21/02 ,  G02B21/26 ,  G02B21/33 ,  H01J49/40

Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.

公开(公告)号：CA3097002A1

公开(公告)日：2019-10-17

申请号：CA3097002

申请日：2019-04-12

Applicant: FLUIDIGM CANADA INC

Inventor： BARANOV VLADIMIR ,  LOBODA ALEXANDER ,  SULLIVAN MICHAEL

IPC: G01N1/44 ,  G01N1/38 ,  H01J49/04

Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.

公开(公告)号：SG11201503038RA

公开(公告)日：2015-05-28

申请号：SG11201503038R

申请日：2013-10-22

Applicant: FLUIDIGM CANADA INC

Inventor： BARANOV VLADIMIR

IPC: G01N15/10 ,  C12M1/34 ,  C12Q1/00 ,  C40B20/02 ,  C40B20/04 ,  H01J49/40

Abstract: A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.

公开(公告)号：CA2914099A1

公开(公告)日：2014-12-11

申请号：CA2914099

申请日：2014-05-28

Applicant: FLUIDIGM CANADA INC

Inventor： TANNER SCOTT

IPC: H01J49/40 ,  H01J49/06 ,  H03M1/12

Abstract: A mass spectrometer of the type useful in mass cytometry includes an ion detector. A digitizing system for converting analog signals from the ion detector includes two analog-to-digital converters. The analog-to-digital converters are configured to provide an increased dynamic range for a targeted period while limiting the amount of data generated.

公开(公告)号：WO2021127657A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/066488

申请日：2020-12-21

Applicant: FLUIDIGM CANADA INC.

Inventor： CAREW, Adam ,  SANDKUIJL, Daaf ,  LOBODA, Alexander ,  WEEL, Matthew ,  BANDURA, Dmitry

IPC: G01N15/10 ,  H01J49/26 ,  G01N15/00 ,  G01N27/62 ,  H01J49/04 ,  H01J49/10

Abstract: Described herein are systems and methods for imaging mass spectrometry, including imaging mass cytometry. Aspects of the subject application include apparatus and methods for imaging mass spectrometry (IMS) that improve speed of sample acquisition, signal sensitivity, and/or signal stability. Systems and methods described herein may minimize the transfer time and/or may minimize the spread of plumes of sample material ablated from a sample to be transferred to the components of the imaging mass spectrometer or mass cytometer that ionize and analyze the sample material.

公开(公告)号：WO2020055743A1

公开(公告)日：2020-03-19

申请号：PCT/US2019/050203

申请日：2019-09-09

Applicant: FLUIDIGM CANADA INC. ,  THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO

Inventor： CLOSSON, Taunia ,  BARANOV, Vladimir ,  WINNIK, Mitchell A.

IPC: G01N15/10 ,  G01N33/50

Abstract: This disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.