-
公开(公告)号：CA3112257A1
公开(公告)日：2020-03-19
申请号：CA3112257
申请日：2019-09-10
Applicant: FLUIDIGM CANADA INC
Inventor： SANDKUIJL DAAF , LOBODA ALEXANDER , CAREW ADAM , ASKARPOUR KHASHAYAR
Abstract: The invention relates to systems and methods for autofocusing in imaging mass spectrometry. The present invention provides improvements over current IMS and IMG apparatus and methods through an autofocus component including plurality of apertures in the autofocus system, such as a plurality of apertures arranged in 2 dimensions. As a plurality of apertures is used the autofocus system provides redundancy in the event that measurement of focus on the sample from the illuminating radiation passed through one or more of the apertures fails so reducing the number of unsuccessful autofocus attempts.
-
公开(公告)号：CA2913806A1
公开(公告)日：2014-12-04
申请号：CA2913806
申请日：2014-05-22
Applicant: FLUIDIGM CANADA INC
Inventor： BANDURA DMITRY , BARANOV VLADIMIR , LOBODA ALEXANDER , ORNATSKY OLGA , TANNER SCOTT D
IPC: G01N27/62 , G01N33/483
Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.
-
公开(公告)号：CA3193031A1
公开(公告)日：2022-03-24
申请号：CA3193031
申请日：2021-09-09
Applicant: FLUIDIGM CANADA INC
Inventor： LOBODA ALEXANDER , JONG RAYMOND , SULLIVAN MICHAEL , VOROBIEV SERGUEI , ROTENBERG ROBERT , STRATULATIV EMIL , VORONOV MAXIM , ARMSTRONG MARK
Abstract: Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma in which a sample is atomized an ionized. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle based ICP analysis includes analysis of particles such as cells, beads, or laser ablation plumes, by atomizing and ionizing particles in an ICP torch followed by atomic analysis. In mass cytometry, mass tags of particles are analyzed by mass spectrometry, such as by ICP-MS. Systems and methods of the subject application include one or more of: a demountable ICP torch holder assembly, an external ignition device; an ICP load coil comprising an annular fin, particle suspension sample introduction fluidics, and ICP analyzers thereof.
-
公开(公告)号：SG11201509713RA
公开(公告)日：2015-12-30
申请号：SG11201509713R
申请日：2014-05-22
Applicant: FLUIDIGM CANADA INC
Inventor： BANDURA DMITRY , BARANOV VLADIMIR , LOBODA ALEXANDER , ORNATSKY OLGA , TANNER SCOTT
IPC: G01N27/62 , G01N33/483
-
公开(公告)号：CA3140663A1
公开(公告)日：2020-12-24
申请号：CA3140663
申请日：2020-06-17
Applicant: FLUIDIGM CANADA INC
Inventor： CORKUM PAUL , LOBODA ALEXANDER , RAYNER DAVID M
Abstract: Embodiments of the present invention relate to replacement of the previous ICP-based ionisation system with a new laser ionisation system, providing improved mass spectrometer-based apparatus and methods for using them to analyse samples, in particular the use of mass spectrometry mass cytometry, imaging mass spectrometry and imaging mass cytometry, for the analysis of biological samples. Accordingly, embodiments of the present invention provide an apparatus, for example a mass cytometer, comprising: 1 ) a sampler; 2) a laser ionisation system to receive material removed from the sample by the sampler, wherein the laser ionisation system comprises an ionisation system conduit and a pulsed laser adapted to ionise sample material passing through or exiting the ionisation system conduit; and 3) a mass spectrometer to receive elemental ions from said ionisation system and to analyse said elemental ions.
-
Patent Agency Ranking
公开(公告)号：CA3134973A1
公开(公告)日：2020-10-01
申请号：CA3134973
申请日：2020-03-27
Applicant: FLUIDIGM CANADA INC
Inventor： LI STEPHEN K H , MAJONIS DANIEL , BARANOV VLADIMIR , ORNATSKY OLGA , ALLO BEDILU , BANDURA DMITRY , THOM COLIN , STELZER GREG , LOBODA ALEXANDER
Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.
-
公开(公告)号：CA3111824A1
公开(公告)日：2020-03-19
申请号：CA3111824
申请日：2019-09-10
Applicant: FLUIDIGM CANADA INC
Inventor： SANDKUIJL DAAF , LOBODA ALEXANDER , CAREW ADAM , LAKSHMAN MINALINI
Abstract: This disclosure relates to systems and methods for high speed modulation sample imaging. Disclosed herein are systems and methods for performing imaging mass cytometry, including analysis of labelling atoms by elemental (e.g., atomic) mass spectrometry. Aspects include a sampling system having, and method of using, a femtosecond (fs) laser and/or laser scanning. Alternatively or in addition, aspects include systems and methods for co-registering other imaging modalities with imaging mass cytometry.
-
公开(公告)号：CA3104126A1
公开(公告)日：2019-12-26
申请号：CA3104126
申请日：2019-06-18
Applicant: FLUIDIGM CANADA INC , CORKUM PAUL
Inventor： CORKUM PAUL , LOBODA ALEXANDER
Abstract: The present invention relates to the high resolution imaging of samples using imaging mass spectrometry (IMS) and to the imaging of biological samples by imaging mass cytometry (IMCTM) in which labelling atoms are detected by IMS. LA-ICP-MS (a form of IMS in which the sample is ablated by a laser, the ablated material is then ionised in an inductively coupled plasma before the ions are detected by mass spectrometry) has been used for analysis of various substances, such as mineral analysis of geological samples, analysis of archaeological samples, and imaging of biological substances. However, traditional LA-ICP-MS systems and methods may not provide high resolution. Described herein are methods and systems for high resolution IMS and IMC.
-
公开(公告)号：CA3097002A1
公开(公告)日：2019-10-17
申请号：CA3097002
申请日：2019-04-12
Applicant: FLUIDIGM CANADA INC
Inventor： BARANOV VLADIMIR , LOBODA ALEXANDER , SULLIVAN MICHAEL
Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.
-
公开(公告)号：EP3004860A4
公开(公告)日：2017-02-15
申请号：EP14804873
申请日：2014-05-22
Applicant: FLUIDIGM CANADA INC
Inventor： BANDURA DMITRY , BARANOV VLADIMIR , LOBODA ALEXANDER , ORNATSKY OLGA , TANNER SCOTT
IPC: G01N27/62 , G01N33/483
CPC classification number: G01N33/54366 , G01N15/1031 , G01N15/1404 , G01N15/1459 , G01N27/622 , G01N27/624 , G01N33/483 , G01N33/532 , G01N33/54313 , G01N2015/1006 , G01N2458/15
Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.
-
-
-
-
-
-
-
-
-
Search Results
10
records
(took 0.024 seconds)
