公开(公告)号：CA3134973A1

公开(公告)日：2020-10-01

申请号：CA3134973

申请日：2020-03-27

Applicant: FLUIDIGM CANADA INC

Inventor： LI STEPHEN K H ,  MAJONIS DANIEL ,  BARANOV VLADIMIR ,  ORNATSKY OLGA ,  ALLO BEDILU ,  BANDURA DMITRY ,  THOM COLIN ,  STELZER GREG ,  LOBODA ALEXANDER

IPC: A61K9/19 ,  G01N15/10 ,  G01N27/62 ,  G01N33/50 ,  G01N33/53 ,  G01N33/532

Abstract: A lyophilized antibody panel is disclosed for interrogation using elemental analysis. The antibody panel includes multiple antibodies each element-tagged or element-labelled with one or more isotopes such that each different antibody is isotopically distinguishable from the other antibodies. Each element tag can include one or more unique isotopes or unique combinations of isotopes. The set of element-tagged antibodies can be lyophilized in admixture. Thus, the lyophilized element-tagged antibody panel can be easily and efficiently resuspended and mixed with a sample prior to interrogation with an elemental analyzer, such as a mass spectrometer. This lyophilized element-tagged antibody panel can provide the benefits of an element-tagged assay while also being easy to use and remaining stable for long durations.

公开(公告)号：CA3098578A1

公开(公告)日：2019-10-31

申请号：CA3098578

申请日：2019-04-26

Applicant: FLUIDIGM CANADA INC

Inventor： ZABINYAKOV NIKITA ,  LOBODA ALEXANDER V ,  ORNATSKY OLGA ,  BARANOV VLADIMIR ,  MAVROPOULOS ANASTASIA ,  MAJONIS DANIEL ,  ALLO BEDILU ,  CLOSSON TAUNIA

IPC: C12M1/00 ,  C12M1/34 ,  G01N35/00 ,  G01N35/10 ,  H01J49/00 ,  H01J49/02

Abstract: Embodiments of the present invention relate to reagents and their use for elemental imaging mass spectrometry of biological samples. The embodiments comprising methods for quantifying one or more analytes within a sample, comprising the steps of: (a) providing the sample, wherein the one or more analytes are immobilized to a sample carrier, wherein the sample has been labelled with one or more mass tags comprising one or more labelling atoms, (b) performing mass cytometry on the sample to determine the level of the one or more labelling atoms, wherein the level of the one or more labelling atoms corresponds to the copy number of the one or more analytes.

公开(公告)号：CA3145861A1

公开(公告)日：2021-02-11

申请号：CA3145861

申请日：2020-08-07

Applicant: FLUIDIGM CANADA INC ,  GOVERNING COUNCIL UNIV TORONTO

Inventor： LIU PENG ,  MAJONIS DANIEL ,  BARANOV VLADIMIR ,  WINNIK MITCHELL A ,  CHO HYUNGJUN

IPC: A61K51/04

Abstract: A new class of mass-tag polymers is provided, which include enriched metal isotopes such as zirconium and hafnium mass tags. The chemistry of these new mass tags are different from that of lanthanide mass tags, and opens up new mass channels that can be used in mass cytometry. These polymers may be used for mass cytometry, therapeutic delivery of a radioactive isotope, or screening of a therapeutic isotope. Aspects include a kit, method of making, and method of using a polymer, isotopic composition, or both. A kit may include a polymer. The polymer may include pendant groups that chelate an enriched isotope, such as zirconium and/or hafnium. The kit may include an isotopic composition comprising an enriched zirconium or hafnium isotope. Polymers may be conjugated to a biologically active material. Aspects may also include making a kit. Aspects include use of a kit, such as for mass cytometry.