ENHANCED PROCESSES AND REAGENTS FOR HOST ENGINEERING

    公开(公告)号:US20190284564A1

    公开(公告)日:2019-09-19

    申请号:US16337491

    申请日:2017-09-27

    Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided. In particular, a host Cupriavidus necator cell is provided in which a chromosomal gene encoding a restriction endonuclease has been disrupted, leading to an increase in transformation efficiency.

    RESINS FOR IMPROVED FLOW IN INJECTION MOLDING
    138.
    发明申请

    公开(公告)号:US20190153155A1

    公开(公告)日:2019-05-23

    申请号:US16094961

    申请日:2017-04-13

    Inventor: John F. BUZINKAI

    Abstract: Resins for improved flow in injection molding, molded articles made from the same, and methods of using the same. A molded article formed from a polymer feed includes nylon-6,6. The molded article also includes a thin elongated profile including a thickness of up to 1.5 mm and a flow length of at least 25 cm. An absolute value of ARV of the molded article as compared to the polymer feed is from 0 to 5, the RV determined according to ASTM D789.

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