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公开(公告)号：KR1020130068007A
公开(公告)日：2013-06-25
申请号：KR1020110135113
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/248 , A23K10/14 , C11D3/38636 , C12N15/67 , C12P7/649
Abstract: PURPOSE: A product of cel10-KG41 gene, endoxylanase, is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoxylanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes endoxylanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoxylanase from the host cell comprises a step of overexpressing endoxylanase by transducing the recombinant vector to the host cells.
Abstract translation: 目的：提供cel10-KG41基因（内切木聚糖酶）的产品，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料。 构成：源自黑山羊的反刍动物胃微生物的内切木聚糖酶蛋白具有序列号2的氨基酸序列。基因编码内切木聚糖酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切木聚糖酶的方法包括通过将重组载体转导至宿主细胞而过表达内切木聚糖酶的步骤。
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公开(公告)号：KR1020130068006A
公开(公告)日：2013-06-25
申请号：KR1020110135112
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2445 , A23K10/14 , C07K16/40 , C11D3/386 , C12N15/67 , C12P7/649 , C12Y302/01021 , Y02E50/13
Abstract: PURPOSE: A protein of cel3-KG39 gene, beta-glucosidase, which is acquired by searching glucosidase gene from a ruminant stomach microorganism of a black goat is provided to be used for a feed additive and a detergent composition, and to develop biofuel. CONSTITUTION: A beta-glucosidase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes beta-glucosidase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of beta-glucosidase in the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing beta-glucosidase. [Reference numerals] (A) Front domain area; (B) Rear domain area
Abstract translation: 目的：提供通过从黑山羊的反刍动物胃微生物中搜索葡糖苷酶基因获得的cel3-KG39基因的β-葡萄糖苷酶蛋白，用于饲料添加剂和洗涤剂组合物，并开发生物燃料。 构成：来自黑山羊的反刍动物胃微生物的β-葡糖苷酶蛋白具有序列号2的氨基酸序列。基因编码β-葡糖苷酶蛋白。 重组载体含有该基因。 宿主细胞被重组载体转化。 用于在宿主细胞中产生大量β-葡糖苷酶的方法包括：将重组载体转导至宿主细胞的步骤; 以及过表达β-葡糖苷酶的步骤。 （附图标记）（A）前域区域; （B）后域区域
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公开(公告)号：KR1020130068005A
公开(公告)日：2013-06-25
申请号：KR1020110135111
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2437 , A23K10/14 , C11D3/38645 , C12N15/67 , C12P1/00 , Y02E50/10
Abstract: PURPOSE: A product of cel-KG35 gene, endoglucanase, is provided to be used for a feed additive and a detergent composition, and to develop biofuel with high efficiency. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase in the host cell comprises: a step of transducing the recombinant vector to the host cell; and a step of overexpressing endoglucanase.
Abstract translation: 目的：提供cel-KG35基因，内切葡聚糖酶的产品，用于饲料添加剂和洗涤剂组合物，并以高效率开发生物燃料。 构成：来自黑山羊的反刍动物胃微生物的内切葡聚糖酶蛋白具有序列号2的氨基酸序列。基因编码内切葡聚糖酶蛋白质。 重组载体含有该基因。 宿主细胞被重组载体转化。 用于在宿主细胞中产生大量内切葡聚糖酶的方法包括：将重组载体转导至宿主细胞的步骤; 以及过表达内切葡聚糖酶的步骤。
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公开(公告)号：KR1020130067999A
公开(公告)日：2013-06-25
申请号：KR1020110135105
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A product of Cel9-KG10 gene, endoglucanase, is provided to be used as a feed additive and a detergent composition and to efficiently develop biofuel by finding a cellulase gene from the microorganism. CONSTITUTION: An endoglucanase protein derived from a ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. The gene encodes the endoglucanase protein. A recombinant vector contains the gene. A host cell is transformed by the recombinant vector. A method for producing a large amount of endoglucanase from the host cell comprises a step of transducing the recombinant vector to the host cell and overexpressing endoglucanase.
Abstract translation: 目的：提供Cel9-KG10基因，内切葡聚糖酶的产物，用作饲料添加剂和洗涤剂组合物，并通过从微生物中发现纤维素酶基因有效地开发生物燃料。 构成：来自黑山羊的反刍动物胃微生物的内切葡聚糖酶蛋白具有序列号2的氨基酸序列。该基因编码内切葡聚糖酶蛋白质。 重组载体含有该基因。 宿主细胞被重组载体转化。 从宿主细胞产生大量内切葡聚糖酶的方法包括将重组载体转导至宿主细胞并过量表达内切葡聚糖酶的步骤。
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Patent Agency Ranking135.发明公开흑염소 반추위 미생물 유래의 엔도―1，4―베타―자일라나아제 ｃｅｌ１０－ＫＧ０４ 유전자 및 이의 용도 有权CEL10-KG04来自黑山羊发酵细菌微生物的内切-1,4-β-XYLANASE基因及其用途
公开(公告)号：KR1020130067997A
公开(公告)日：2013-06-25
申请号：KR1020110135103
申请日：2011-12-15
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N9/2482 , A23K10/14 , C11D3/38636 , C12N15/67 , C12P7/649 , C12Y302/01008
Abstract: PURPOSE: Endo-1,4-beta-xylanase which is a product of cel10-KG04 gene is provided to be used for a feed additive and a detergent composition, and to develop biofuel. CONSTITUTION: Endo-1,4-beta-xylanase derived from ruminant stomach microorganism of a black goat has an amino acid sequence of sequence number 2. A gene encodes endo-1,4-beta-xylanase. A recombinant vector contains the gene. A host cell is transformed with the recombinant vector. A method for producing a large amount of endo-1,4-beta-xylanase from the host cell comprises a step of transducing a recombinant vector to the host cell and overexpressing endo-1,4-beta-xylanase.
Abstract translation: 目的：提供作为cel10-KG04基因产物的Endo-1,4-β-木聚糖酶用于饲料添加剂和洗涤剂组合物，并开发生物燃料。 构成：来源于黑山羊的反刍动物胃微生物的内切-1,4--β-木聚糖酶具有序列号2的氨基酸序列。一个基因编码内切-1,4-β-木聚糖酶。 重组载体含有该基因。 用重组载体转化宿主细胞。 用于从宿主细胞产生大量内切-1,4-β-木聚糖酶的方法包括将重组载体转导到宿主细胞并过量表达内切-β-β-木聚糖酶的步骤。
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公开(公告)号：KR1020110030808A
公开(公告)日：2011-03-24
申请号：KR1020090088414
申请日：2009-09-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6858 , C12N15/11 , C12Q2537/143
Abstract: PURPOSE: A microsatellite marker and a method for identifying dog individual using the same are provided to perform multiplex PCR and to perform parental testing. CONSTITUTION: A microsatellite marker which is able to distinguish dog individual is a combination of one or more sets among: a first set having FH3005, FH2537, FH3921, FH3381, FH3116, FH3372, REN62M06, REN277O05, REN51C16, FH3399, and FH3027; a second set having FH2097, REN204K13, FH1014, FH3058, FH2834, FH2712, REN112C08, FH2998, FH2584, and REN197E16; and a third set having FH2054, REN181K04, FH2079, REN01O23, FH2582, and FH2790. A method for identifying dog individual comprises: a step of performing multiple PCR using microsatellite marker; and a step of detecting allele through electrophoresis and determining genotype.
Abstract translation: 目的：提供微卫星标记和用于鉴定使用其的狗个体的方法以进行多重PCR并进行亲本测试。 构成：能够区分狗个体的微卫星标记是：具有FH3005，FH2537，FH3921，FH3381，FH3116，FH3372，REN62M06，REN277O05，REN51C16，FH3399和FH3027的第一组中的一组或多组的组合; 具有FH2097，REN204K13，FH1014，FH3058，FH2834，FH2712，REN112C08，FH2998，FH2584和REN197E16的第二组; 第三组具有FH2054，REN181K04，FH2079，REN01O23，FH2582和FH2790。 用于鉴定狗个体的方法包括：使用微卫星标记进行多个PCR的步骤; 以及通过电泳检测等位基因并确定基因型的步骤。
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公开(公告)号：KR1020100111143A
公开(公告)日：2010-10-14
申请号：KR1020090029553
申请日：2009-04-06
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6827 , C12N15/11 , C12Q1/6844 , C12Q2600/156
Abstract: PURPOSE: A method for distinguishing bovine species by detecting mutation of fixed gene is provided to accurately identify bovine species. CONSTITUTION: A SNP(single nucleotide polymorphism) is used for distinguishing bovine species using information. A method for distinguishing bovine species comprises: a step of amplifying bovine DNA containing selected SNP through PCR-RFLP; a step of treating the nucleic acid with specific restriction enzyme.
Abstract translation: 目的：提供通过检测固定基因突变来区分牛种的方法，准确识别牛种。 构成：使用SNP（单核苷酸多态性）来区分使用信息的牛种。 鉴别牛种的方法包括：通过PCR-RFLP扩增含有所选SNP的牛DNA的步骤; 用特异性限制酶处理核酸的步骤。
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公开(公告)号：KR1020020068225A
公开(公告)日：2002-08-27
申请号：KR1020010008523
申请日：2001-02-20
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: Provided is an analysis method for the discrimination by PCR-RFLP of the bovine melanocortin receptor 1(MC1R) gene to prevent unfair distribution of beef meat. CONSTITUTION: The analysis method characteristically comprises the steps of: performing PCR with a variable region of MC1R gene as a primer; cleaving PCR products, BsrF I and MspAl I with restriction enzymes; and determining genotype by electrophoresis.
Abstract translation: 目的：提供牛黑皮质素受体1（MC1R）基因的PCR-RFLP鉴别分析方法，防止牛肉不正当分布。 构成：分析方法的特点是包括以MC1R基因的可变区作为引物进行PCR; 用限制酶切割PCR产物，BsrF I和MspA1 I; 并通过电泳确定基因型。
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公开(公告)号：KR100282246B1
公开(公告)日：2001-04-02
申请号：KR1019980005869
申请日：1998-02-25
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 소 육종을 조기에 간편하게 선발하도록 하는 소 육질 관련 DNA 표지인자를 이용한 비육우 조기선발 방법에 관한 것으로, 소 육질관련 DNA 표지인자는 NCBI GenBank에 등록번호 제U50365호의 유전정보 3006호부터 3365번까지의 exon 2에서 염기치환의 결과 생긴 유전자형(M 형태)이다.
본 발명에 따라 미성숙 소들로부터 요구되는 육질의 비육우를 육종하도록 조기에 선발할 수 있어서 비육우를 보다 경제적이고 효율적으로 육종할 수 있게 되는 효과가 있다.
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