公开(公告)号：KR1020110030808A

公开(公告)日：2011-03-24

申请号：KR1020090088414

申请日：2009-09-18

Applicant: 대한민국(농촌진흥청장)

Inventor： 최봉환 ,  김태헌 ,  조용민 ,  문희주 ,  장홍철

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6858 ,  C12N15/11 ,  C12Q2537/143

Abstract: PURPOSE: A microsatellite marker and a method for identifying dog individual using the same are provided to perform multiplex PCR and to perform parental testing. CONSTITUTION: A microsatellite marker which is able to distinguish dog individual is a combination of one or more sets among: a first set having FH3005, FH2537, FH3921, FH3381, FH3116, FH3372, REN62M06, REN277O05, REN51C16, FH3399, and FH3027; a second set having FH2097, REN204K13, FH1014, FH3058, FH2834, FH2712, REN112C08, FH2998, FH2584, and REN197E16; and a third set having FH2054, REN181K04, FH2079, REN01O23, FH2582, and FH2790. A method for identifying dog individual comprises: a step of performing multiple PCR using microsatellite marker; and a step of detecting allele through electrophoresis and determining genotype.

Abstract translation: 目的：提供微卫星标记和用于鉴定使用其的狗个体的方法以进行多重PCR并进行亲本测试。 构成：能够区分狗个体的微卫星标记是：具有FH3005，FH2537，FH3921，FH3381，FH3116，FH3372，REN62M06，REN277O05，REN51C16，FH3399和FH3027的第一组中的一组或多组的组合; 具有FH2097，REN204K13，FH1014，FH3058，FH2834，FH2712，REN112C08，FH2998，FH2584和REN197E16的第二组; 第三组具有FH2054，REN181K04，FH2079，REN01O23，FH2582和FH2790。 用于鉴定狗个体的方法包括：使用微卫星标记进行多个PCR的步骤; 以及通过电泳检测等位基因并确定基因型的步骤。

公开(公告)号：KR101156047B1

公开(公告)日：2012-06-27

申请号：KR1020090088414

申请日：2009-09-18

Applicant: 대한민국(농촌진흥청장)

Inventor： 최봉환 ,  김태헌 ,  조용민 ,  문희주 ,  장홍철

IPC: C12Q1/68 ,  C12N15/11

Abstract: PURPOSE: A microsatellite marker and a method for identifying dog individual using the same are provided to perform multiplex PCR and to perform parental testing. CONSTITUTION: A microsatellite marker which is able to distinguish dog individual is a combination of one or more sets among: a first set having FH3005, FH2537, FH3921, FH3381, FH3116, FH3372, REN62M06, REN277O05, REN51C16, FH3399, and FH3027; a second set having FH2097, REN204K13, FH1014, FH3058, FH2834, FH2712, REN112C08, FH2998, FH2584, and REN197E16; and a third set having FH2054, REN181K04, FH2079, REN01O23, FH2582, and FH2790. A method for identifying dog individual comprises: a step of performing multiple PCR using microsatellite marker; and a step of detecting allele through electrophoresis and determining genotype.