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公开(公告)号：KR101149444B1
公开(公告)日：2012-06-27
申请号：KR1020090029553
申请日：2009-04-06
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A method for distinguishing bovine species by detecting mutation of fixed gene is provided to accurately identify bovine species. CONSTITUTION: A SNP(single nucleotide polymorphism) is used for distinguishing bovine species using information. A method for distinguishing bovine species comprises: a step of amplifying bovine DNA containing selected SNP through PCR-RFLP; a step of treating the nucleic acid with specific restriction enzyme.
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Patent Agency Ranking
公开(公告)号：KR1020110030808A
公开(公告)日：2011-03-24
申请号：KR1020090088414
申请日：2009-09-18
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6858 , C12N15/11 , C12Q2537/143
Abstract: PURPOSE: A microsatellite marker and a method for identifying dog individual using the same are provided to perform multiplex PCR and to perform parental testing. CONSTITUTION: A microsatellite marker which is able to distinguish dog individual is a combination of one or more sets among: a first set having FH3005, FH2537, FH3921, FH3381, FH3116, FH3372, REN62M06, REN277O05, REN51C16, FH3399, and FH3027; a second set having FH2097, REN204K13, FH1014, FH3058, FH2834, FH2712, REN112C08, FH2998, FH2584, and REN197E16; and a third set having FH2054, REN181K04, FH2079, REN01O23, FH2582, and FH2790. A method for identifying dog individual comprises: a step of performing multiple PCR using microsatellite marker; and a step of detecting allele through electrophoresis and determining genotype.
Abstract translation: 目的：提供微卫星标记和用于鉴定使用其的狗个体的方法以进行多重PCR并进行亲本测试。 构成：能够区分狗个体的微卫星标记是：具有FH3005，FH2537，FH3921，FH3381，FH3116，FH3372，REN62M06，REN277O05，REN51C16，FH3399和FH3027的第一组中的一组或多组的组合; 具有FH2097，REN204K13，FH1014，FH3058，FH2834，FH2712，REN112C08，FH2998，FH2584和REN197E16的第二组; 第三组具有FH2054，REN181K04，FH2079，REN01O23，FH2582和FH2790。 用于鉴定狗个体的方法包括：使用微卫星标记进行多个PCR的步骤; 以及通过电泳检测等位基因并确定基因型的步骤。
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公开(公告)号：KR1020100111143A
公开(公告)日：2010-10-14
申请号：KR1020090029553
申请日：2009-04-06
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6827 , C12N15/11 , C12Q1/6844 , C12Q2600/156
Abstract: PURPOSE: A method for distinguishing bovine species by detecting mutation of fixed gene is provided to accurately identify bovine species. CONSTITUTION: A SNP(single nucleotide polymorphism) is used for distinguishing bovine species using information. A method for distinguishing bovine species comprises: a step of amplifying bovine DNA containing selected SNP through PCR-RFLP; a step of treating the nucleic acid with specific restriction enzyme.
Abstract translation: 目的：提供通过检测固定基因突变来区分牛种的方法，准确识别牛种。 构成：使用SNP（单核苷酸多态性）来区分使用信息的牛种。 鉴别牛种的方法包括：通过PCR-RFLP扩增含有所选SNP的牛DNA的步骤; 用特异性限制酶处理核酸的步骤。
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公开(公告)号：KR101008941B1
公开(公告)日：2011-01-17
申请号：KR1020070100513
申请日：2007-10-05
Applicant: 대한민국(농촌진흥청장) , 경상대학교산학협력단
IPC: C12Q1/68
Abstract: 본 발명은 여러 소 품종 내에서 세 개의 멀티플렉싱 피씨알 시스템(multiplexing PCR system)을 이용하여 다중의 서로 다른 유전자 좌위를 동시에 다중 증폭시킨 후, 각각의 초위성체 좌위(microsatellite loci)의 대립유전자 형질을 측정하여 한우 및 수입우를 판별하는 방법에 관한 것이다.
한우, 품종식별, 멀티플렉싱 피씨알 시스템, 수입우육-
公开(公告)号：KR101156047B1
公开(公告)日：2012-06-27
申请号：KR1020090088414
申请日：2009-09-18
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A microsatellite marker and a method for identifying dog individual using the same are provided to perform multiplex PCR and to perform parental testing. CONSTITUTION: A microsatellite marker which is able to distinguish dog individual is a combination of one or more sets among: a first set having FH3005, FH2537, FH3921, FH3381, FH3116, FH3372, REN62M06, REN277O05, REN51C16, FH3399, and FH3027; a second set having FH2097, REN204K13, FH1014, FH3058, FH2834, FH2712, REN112C08, FH2998, FH2584, and REN197E16; and a third set having FH2054, REN181K04, FH2079, REN01O23, FH2582, and FH2790. A method for identifying dog individual comprises: a step of performing multiple PCR using microsatellite marker; and a step of detecting allele through electrophoresis and determining genotype.
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公开(公告)号：KR1020090035302A
公开(公告)日：2009-04-09
申请号：KR1020070100513
申请日：2007-10-05
Applicant: 대한민국(농촌진흥청장) , 경상대학교산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q2537/143
Abstract: A method for discrimination between Korean beef cattle and imported cattle using a multiplexing PCR and a primer therefore are provided to distinguish species of cattle by analyzing gene loci rapidly and accurately. A method for discrimination between Korean beef cattle and imported cattle using a multiplexing PCR comprises the following steps of: obtaining DNA of cattle having two pairs of primers and a plurality of gene loci; amplifying a base sequence comprising each locus of a DNA sample into a multiplexing PCR; and measuring characteristic form and quality of an allele of each microsatellite locus.
Abstract translation: 提供了使用复用PCR和引物来区分韩国肉牛和进口牛的方法，以通过快速且准确地分析基因位点来区分牛的种类。 使用复用PCR鉴别韩国肉牛和进口牛的方法包括以下步骤：获得具有两对引物和多个基因座的牛的DNA; 将包含DNA样品的每个位点的碱基序列扩增到多重PCR中; 并测量每个微卫星位点等位基因的特征形式和质量。
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